FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_S10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_S10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4540513
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACGAACGGTAATCTCGTATGCC559361.2319312817736674No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAACGGTACTCTCGTATGCC258590.5695171448688727No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG228670.5036215070852126No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGAACGGTAATCTCGTATGC213580.47038737693295885No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGAACGGTACTCTCGTATGC121850.26836174678940466No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC107060.23578833493043627No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA80550.17740286174711975No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA67680.14905804696517772No Hit
GCAGGAAGGGGGGTCGCCGTGGTCGCCGTCTGTGAACAAGATTCCTCAAA49510.1090405423351943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAACG254.4447068E-544.00036220
GTATGCC116650.040.62129644
AGACACG12850.038.6929320
TCCGACG4050.036.9357341
GACACGT13350.036.9141621
ACGTGCC13600.036.72048624
CGGCCTA14200.036.56247310
CTACTCG28850.036.534812
TACTCGG29200.036.2476123
ACACGTG13850.035.5815222
TACGTCG505.955917E-835.19757516
CACGTGC14200.035.16930423
GCTACTC60700.034.947031
GTCGAAT4800.034.83247410
ACGGGCA7350.034.7290275
CCGTAAC6850.034.68374641
TATACCG4400.034.49914634
CGACGGC4400.034.49762343
TGCGGCC15850.034.421868
CGTACTA3900.034.40788742
Produced by FastQC (version 0.11.5)