FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTCACAATACGTTTACAGCTGCCTTTCCTTCACATACTTTTCTAATTC11.0526315789473684No Hit
ACTGCCAGCCCCTAACTGAAATCTGAAGCTTTTTATCGCTTATATTTCCT11.0526315789473684No Hit
CCATGATGTCTTCGTTAGAAAAGTAAAAATGCTGAAGAAGCCCAAGTTTG11.0526315789473684No Hit
GTGTGCCTGGAGCCAGTCCCACCACGCTCGCGTTTCCTCCTGTAGTGCTC11.0526315789473684No Hit
CTGACCAGTGGGCTTTGGCTTCTCCAGCCGCCTCCAGTGCTGCGTGATTT11.0526315789473684No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCAACCTAGTCTCGTATGCCG11.0526315789473684No Hit
GGACCAAAGCACCCAAGATTCAGCGTCTTGTTACTCCACGTGTCCTGCAG11.0526315789473684No Hit
CTGGGAAGATGGGGCTTAGGTAACAGCCAAACCTGGCTGTCAGCTGTGTG11.0526315789473684No Hit
CCAGTGAGTGTCCTGCAGCCCTTATTCCCTCCATAGAAAGCATCCTCAGA11.0526315789473684No Hit
GGTGATGCGCGTAGTCAGCGCCCGTCTGCCTCGTCTTGTATACTTGTTAG11.0526315789473684No Hit
CCCGTATTTACCCTATAGCACACCCTCTACCCCCTCTAGAGCCAAAAAAA11.0526315789473684No Hit
GTCTCTAAGAAAATTAAAAAAAAATTACCCAGACATGATGGCATATACCT11.0526315789473684No Hit
TCTATTAAAGAAGTAATTGGCCTTTCTGAGCTGATTTTTCCATCTTTTGT11.0526315789473684No Hit
GCCTGCGTGAGAACTGTCTTCTTTGTAGGTATATTTAATATTTGTTGGAC11.0526315789473684No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCAACCTACTCTCGTATGCC11.0526315789473684No Hit
GCAGGAAGGGGGGTCGCCGTGGTCGCCGTCTGTGAACAAGATTCCTCAAA11.0526315789473684No Hit
CAAAATGAAGAAGTATGGCATTTCAAATATAGATACAACCATTGAAGGAA11.0526315789473684No Hit
GCATGGGATGGATGACCCGGAGAGGGAAGTATTAGAGTGGAGGTTTGACA11.0526315789473684No Hit
CCTGAGTGATTTTCTACTGTACTAATGTTTAGATGATAGCAGATAAAACA11.0526315789473684No Hit
GCACAATGACGTGTTGGAGGGGGCTAAATATCTAGCAAAAAAATAGGAAA11.0526315789473684No Hit
GAAATCATGAGCATTTCACTTTTTCTAAGTCAAGTATTTCATAAGGATTT11.0526315789473684No Hit
GCTAGTGCTGACGATGTTCTGTACAACTTAACTCACTGGCGAGAATACAG11.0526315789473684No Hit
CCCTCAAGGGCATCCTGGGATACACTGAGCACCAGGTGGTCTCCTCTGAC11.0526315789473684No Hit
CCTCTTGAAAAGGGTCAGATGTTCCACCACGAGACCTCGACATCCCTCAC11.0526315789473684No Hit
GGGACTCAGGTACTTTCCCCAGTTGGGACTGAACTTTCTCATCAGAGAAT11.0526315789473684No Hit
GCCATCTTCACCATTCAACTAAACTTGAATGGGATATTATCAAACTTAAA11.0526315789473684No Hit
CCTTTTCTTCTTTATTTTCATTAGTTGTACATTGATTTCAGTGTCAACAC11.0526315789473684No Hit
CCTGCTGAGGGGGCTCTTTCCCTCATGTATACTTCAAGTAAGATCAAGAA11.0526315789473684No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.0526315789473684No Hit
CCCCCAAAGCAATGTGAGTCCCAGAGCCCGCTTTTGTTCTTCCCCACAAT11.0526315789473684No Hit
TCTATTTAGGTGGGAATAGTTGTGGATGTACTAAGAGTAATGAAATAAAA11.0526315789473684No Hit
TAGTATTATTCATTTATAAAAATAAATGAATAATACTATTCCATTGTATG11.0526315789473684No Hit
CGCTACTAAGGGGCTTGTGTTCTGCTCCATACCTTTTCTCTTTTCTTTCT11.0526315789473684No Hit
GCAAGCATTTTGAACTGGGAGGAGATAAGAAGAGAAAGGGCCAAGTGATC11.0526315789473684No Hit
GTGCTATCTGCTGTGCCATTCGTAGGATGGGTGAGTCAGATGATTCCATT11.0526315789473684No Hit
TTTACTAAGTGGGATATGCTAATTATACTTTTTGTTGTGGATAAACAAAA11.0526315789473684No Hit
GCAATCACCAGCCTTGAAAGGCAGCAGGGTGCCCAGGTGAAGCTGGACTG11.0526315789473684No Hit
GATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACT11.0526315789473684No Hit
CTAGTATGGCTTGTTGGCAAAGAGTGCTACACCGTTTCAATGAAACAATG11.0526315789473684No Hit
GGCGCGGCCTGCCTTTCCCATCTGTCTATCTATCTGGCTGGCAGGGAAGC11.0526315789473684No Hit
GCTACTCGGGAGGCTGAGGCAGCCTGGTGTGAAGCCGGGAGGCAGAGCTT11.0526315789473684No Hit
GTCCTTCTGGTTGTCTTTGCGTGGGAGGGAGAGGAGCTCAGGACGTAACC11.0526315789473684No Hit
TTTATTCCGGGCGTCTTGGTAGTTTCTGGTGGGATTCAGTGGGTGAGAGG11.0526315789473684No Hit
CCTTATCAGGAGTTCACTGACCACCTCGTCAAGACCCACACCAGAGTCTC11.0526315789473684No Hit
TTTATTGGGTAGGCCTGATTTTTGATGATGTCCTTAAAAGGCTAGCCTGA11.0526315789473684No Hit
CGTTGCTGGGGAAGCTTTAAAAAAAAAAAAAGATGTCCCACTGAGATTCG11.0526315789473684No Hit
CCATTGTGGGATGATTAAACATGGTATTTAACTGTGCAAAAAAAAAAAAG11.0526315789473684No Hit
GCAAGATTTTAGGAGTTTGAATTGGTTCTTGTATGTTCTCATTCTACATA11.0526315789473684No Hit
CCCAAAGCTGGTGTCAAGCCAACCGCATGGCCTCCCGGACTGTTACAAAA11.0526315789473684No Hit
CCGACGGGAGGCCAAGGTCAAGTTTGAAGAGAGATACAAGACAGGCAAGA11.0526315789473684No Hit
GCTATTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACACAGGAGGCAGAC11.0526315789473684No Hit
CTTATACTATTGTTTGTGATTAAAAGTGCTTCAACCCGAAAAAAAAAAAA11.0526315789473684No Hit
GCCCTTGAGAAGGATTGTGAGGAGGTTGGTGAGGATTCTGTAGAAGGAGA11.0526315789473684No Hit
GGGTGCAGTGGCTCATGCCTGTAATCTCAATACTTTGAGGCCAAGGTGGG11.0526315789473684No Hit
TCTGTTATTGGTGGGTTTTTAGTGATTGTAGTGTGTTATTTTCTTGCTGA11.0526315789473684No Hit
CGGAAAGAGACAGTTTTTTTTTTTTTTTTTTTATTTAAAAAGAGATGAAG11.0526315789473684No Hit
GGCGGCAGAGACAGACTCTGTCTCGCAAAAAAAAATAAAAAATAATAATA11.0526315789473684No Hit
TTTTCTCAGAGAATTAAAAACAAAAAAAGTACTCTTGTGAAAAAAAAAAA11.0526315789473684No Hit
GGTTTTGAGAACTTTTCTGGTATTACGTTTATGGCAAATGTATAAAAGAA11.0526315789473684No Hit
GCTCGCGGGCAAGGATGTTAATTTTGAAGTCCCAGAGTTTCAATTGTAAA11.0526315789473684No Hit
GCTAGAATTCAAAAGATAAAAAAGAAAACTATTAAGATATTTGAAATCTG11.0526315789473684No Hit
CCCAAAAATGTTAATCACAGCTGGATTGAAAGAGTATTTGGGAAATGTGG11.0526315789473684No Hit
TCTCTTATACTCATCTCCGAGCGCTCGAGACACGTTCAATCTCGTATGGC11.0526315789473684No Hit
GCTTCAAGGAGGGCAATTTCTAGAAGGGCAGGCTGCAGTGAGACATGAAC11.0526315789473684No Hit
GTTATCTATGCGGGAGTAAGGGGATTTCTTGATACACAGGAGCCGAGCAA11.0526315789473684No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTATGCC11.0526315789473684No Hit
CACAGTCGGGAGGCTGAGGTGGGAGCCTCCCTTGAGCCTGGCAGGTCGAG11.0526315789473684No Hit
GAGTGAATGGGGGGGTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0526315789473684No Hit
ACCTGTCTGACATTGTCCCCTAAAAACAAGTGCAGTGTGGCAGCTGCTGA11.0526315789473684No Hit
GAGTGAATGGGTGAGTATGTAACTAAGGCAAAAAAAAAAAAAAAAAAAAA11.0526315789473684No Hit
TCTCTTATACTCATCTCCGAGCCCACGAGACCGTTCGACTCTCGTATGCC11.0526315789473684No Hit
GCTTCCGGAGGCTCCCAGTGTCCTGGAGGAGAAAAGCATTAGAGGGGGCA11.0526315789473684No Hit
CCTTTGCTCCCTTTTTCCAATTTCTTATTGCATATCATTTTGTTGTAATA11.0526315789473684No Hit
CCACAGGCCATCTCGTTTTCTGAACTTTGTCTTAGACATTAACATGCAAA11.0526315789473684No Hit
CTTAGCATGGAGGACGAAGAAAGCTGTACATTGTTGCTTGAGAGTCTGTA11.0526315789473684No Hit
GACCAGGGCAGGGCTTAGTCTAAAAGTATGAAGCCCAAGGGTGGATGATG11.0526315789473684No Hit
CTCTTATACACATCTCCGAGCCCTCGAGACGCAACCTACTCTCGTATGCC11.0526315789473684No Hit
GGCACCAGGAAGGTGAGATTTTCGACACAGAAAAAGAGAAATATGAGATT11.0526315789473684No Hit
ATGATGCCTGGAACCTTGATTACAGTAATACATCAGCTCTTGTACTATTC11.0526315789473684No Hit
CTCTTATACACATCTCCGAGCCCTCGAGACAGGTGAATCTCGGATGCCGG11.0526315789473684No Hit
GCTTAAGTCAACTATTTTAATAAATTGATGACCAGTTGTCAAAAAAAAAA11.0526315789473684No Hit
GCGTCTACTGGCCTATAATAAATGGGTTAATTTATGTAACAAAAAAAAAA11.0526315789473684No Hit
GCCACTTGGAGGTCTGCAGGACATGTTCTGTAGCCAACATGATAAATATT11.0526315789473684No Hit
GCTATGCAAAGATCTTTGGGCCCAAGGGATTTGGCTATGGCCAAGGAGCA11.0526315789473684No Hit
GCTCAAGATCAGACGGTTAATCATGATTGACTAAATCTGTCTGATGATCG11.0526315789473684No Hit
GGACTGAGGAGGGGTAGGCCTGCATCCACAGAGAGGATCCAGGCCAAGGC11.0526315789473684No Hit
GTTGATAGCTGAGGTTATGGGAGAAGATATCCCAGAAAAAATAAAAGATG11.0526315789473684No Hit
CCTGGGGACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0526315789473684No Hit
GCAAAAAGGGAGCCAAGAAGAAAGTGGTTGATCCATTTTCTAAGAAAGAT11.0526315789473684No Hit
GAAAAAAAGTGGGTAATTGAAGCTTATGGTTGATTAATTTCCTAACAGCA11.0526315789473684No Hit
CTCTTATACACATCTCCGAGCCGACGAGACGCAAGCTAATCTCGTATGCC11.0526315789473684No Hit
GTTAACGGTGAGGTCAATAACTTAGAAATAAACTATTACAGAAATGTAAC11.0526315789473684No Hit
CATTAAATAAAGAGTCTGTTGCCTTAAAAAAAAAAAAAAAAAAAAAAAAA11.0526315789473684No Hit
GCTACTTGGGAGGCTGGCACGGAAGCATTGCGTGCTCCTGGGAGCTGGGA11.0526315789473684No Hit
CCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGA11.0526315789473684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTGT50.044.031
AGCCCGC50.044.025
TGTGAGT50.044.013
CAATGTG50.044.010
TTGTTCT50.044.034
TCCCAGA50.044.019
CCCAGAG50.044.020
CCAAAGC50.044.04
GTTCTTC50.044.036
CCGCTTT50.044.028
CGCTTTT50.044.029
TGTTCTT50.044.035
CCAGAGC50.044.021
GAGCCCG50.044.024
CCCACAA50.044.043
GTGAGTC50.044.014
GCAATGT50.044.09
GAGTCCC50.044.016
CCCGCTT50.044.027
ATGTGAG50.044.012
Produced by FastQC (version 0.11.5)