FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48318
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCC30486.308208121197069No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGCTCTCGTATGCC13322.7567366198932075No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCCGT5481.134152903679788No Hit
CTCTTATACACATCTCCGAGCCCTCGAGACCAGAACTGATCTCGTATGCC4220.8733805207169172No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGATCTGATCTCGTATGCC3080.6374436027981291No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2690.5567283414048595No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCCG2410.4987789229686659No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTCTGCC2290.473943457924583No Hit
CTCTTATACACATCTCCGAGCCCTCGAGACCAGAACTGCTCTCGTATGCC2140.4428991266194793No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAACTGCTCTCGTATGCCGT1830.378740841922265No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGATCTGCTCTCGTATGCC1790.37046235357423735No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGCTCTCGTCTGCC1670.3456268885301544No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGAGCTCGTATGCC1540.31872180139906453No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCACTGATCTCGTATGCC1500.3104433130510369No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGAACTGATCTCGTATGCC1250.2587027608758641No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCACTGCTCTCGTATGCC1240.25663313878885713No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGC1060.21937994122273272No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGAACTGCTCTCGTATGCCG750.15522165652551845No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGCTCTCGTATGC750.15522165652551845No Hit
CTCTTATACACATCTCCGAGCCCTCGAGACCAGATCTGATCTCGTATGCC710.1469431681774908No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC670.13866467982946315No Hit
CCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCCG660.13659505774245623No Hit
CTCTAATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCC650.1345254356554493No Hit
GCCCTTGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGA620.12831656939442856No Hit
TATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCCGTCT600.12417732522041476No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATGTCGTATGCC600.12417732522041476No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGAACTGCTCTCGTATGCC580.12003808104640093No Hit
CTCATATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCC570.11796845895939402No Hit
CTCTTATACACATCTCCGAGCCCACGAGAGCAGAACTGATCTCGTATGCC570.11796845895939402No Hit
CTCTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCCG500.10348110435034563No Hit
ACAAAGGTGCTGCTTTTACAGGGAAGCTTATTCTGTTTTAAACATTGAAA490.10141148226333872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTAC207.781724E-444.036
GAGAAGG207.781724E-444.07
GTACTCC302.477529E-644.039
TCGCGCC254.3796226E-544.029
GATCGCG302.477529E-644.027
GAACCTG254.3796226E-544.034
TCATGCC207.781724E-444.029
ATCATGC254.3796226E-544.028
ATCGCGC254.3796226E-544.028
GTGAACC408.030838E-944.032
TGAACCT302.477529E-644.033
CATGCCA207.781724E-444.030
GCCCTTG207.781724E-444.01
TGAGGCA1250.040.4815
CTCTTAT11450.038.8122251
GCGTGAA404.016365E-738.530
CGTGAAC404.016365E-738.531
TCGGGAG404.016365E-738.56
ATCTGAT1150.038.2608734
TCTCTTA1450.037.9310381
Produced by FastQC (version 0.11.5)