FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15684
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG860.5483295077786279No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCAACCTAATCTCGTATGCC490.3124203009436368No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAACGGTAATCTCGTATGCC310.19765365978066818No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGCC210.13389441469013008No Hit
CCGAGCCCACGAGACGAACGGTAATCTCGTATGCCGTCTTCTGCTTGAAA200.12751849018107625RNA PCR Primer, Index 12 (96% over 28bp)
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC200.12751849018107625No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA190.12114256567202243No Hit
AGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTC170.10839071665391482No Hit
CCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGA170.10839071665391482No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCAAG207.620583E-444.09
GCAAGGT207.620583E-444.011
CAACCTA207.620583E-444.032
ACGCAAC207.620583E-444.029
GGCAAGG207.620583E-444.010
GCTACTC207.620583E-444.01
GCAACCT207.620583E-444.031
GACGCAA207.620583E-444.028
AGACGCA207.620583E-444.027
CGCAACC207.620583E-444.030
GAGACGC302.3709072E-644.026
CTCTTAT556.548362E-1140.01
CTTGGGA301.2433827E-436.6666645
TCTTATA601.6734703E-1036.6666642
AGGCAGA401.7085531E-533.043
GGAGAAT551.2327473E-732.022
TTGGGAG353.0761384E-431.4285726
CTTATAC708.785719E-1031.4285723
AGGCTGA502.1711912E-630.80000111
CGTATGC651.526314E-830.4615443
Produced by FastQC (version 0.11.5)