FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5378
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCC661.2272220156191893No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGCTCTCGTATGCC420.7809594644849386No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG360.669393826701376No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCCGT240.4462625511342506No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC220.4090740052063964No Hit
CCTCTGGGGGTCCTCTCTGAGTCAAATCCAGTGGTGGGTAATTGTACAAT210.3904797322424693No Hit
CCTTTATTGAAATGTTTTCCTTTGTGCTTAACTGGCTGGGCATTCCACAG140.26031982149497956No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA140.26031982149497956No Hit
CTCTTATACACATCTCCGAGCCCTCGAGACCAGAACTGATCTCGTATGCC140.26031982149497956No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA100.1859427296392711No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGATCTGATCTCGTATGCC90.167348456675344No Hit
GCAGGAAGGGGGGTCGCCGTGGTCGCCGTCTGTGAACAAGATTCCTCAAA80.14875418371141688No Hit
GCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAA80.14875418371141688No Hit
GCCCTTGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGA70.13015991074748978No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA70.13015991074748978No Hit
CCCTGGGTGACATGTCAGATCTTTGTACGTAATTAAAAATATTGTGGCAG60.11156563778356265No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCACTGCTCTCGTATGCC60.11156563778356265No Hit
GAGAATGAAGGAGGCTAAGGAGAAGCGCCAGGAACAAATTGCGAAGAGAC60.11156563778356265No Hit
CTCAAAAAGGACACTTCTGATTAAGACGGTTGAAACTAGAGATGGACAGG60.11156563778356265No Hit
CCGTGCGCGAGGGCGACGTGCTCACCCTTTTGGAGTCAGAGCGAGAAGCC60.11156563778356265No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC60.11156563778356265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT302.1081523E-643.934581
TTATACA302.1081523E-643.934584
TGCTCTC207.226828E-443.9345837
CTTATAC302.1081523E-643.934583
AGTGAGC207.226828E-443.9345817
CTGCTCT207.226828E-443.9345836
GCTCTCG207.226828E-443.9345838
TCTTATA302.1081523E-643.934582
TATACAC356.087679E-637.658215
ATACACA356.087679E-637.658216
CGAGACC301.1461603E-436.61215225
AGACCAG301.1461603E-436.61215227
GAGACCA301.1461603E-436.61215226
GTATGCC250.002165993235.14766344
CTCTCGT250.002165993235.14766339
GCAGTGA250.002165993235.14766315
CGTATGC250.002165993235.14766343
GACCAGA250.002165993235.14766328
ATCTCCG457.5478783E-734.4937112
CACATCT457.5478783E-734.493719
Produced by FastQC (version 0.11.5)