FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGCC231.9087136929460582No Hit
CTCTTATACACATCTCCGAGCCCTCGAGACGCCTCAATATCTCGTATGCC60.49792531120331945No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.49792531120331945No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA50.4149377593360996No Hit
TCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTT50.4149377593360996No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCACTATCTCGTATGCC40.33195020746887965No Hit
CCTTCCTGGGGGCCTTGGCTTTGATCTACAATGAAGCCCTCAAGGGCTGA30.24896265560165973No Hit
GCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCAC30.24896265560165973No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATCTCTCGTATGCC30.24896265560165973No Hit
GCTCTAGGAGGGCTGGCAACTTAGAGGTGGGGAGCAGAGAATTCTCTTAT30.24896265560165973No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTATGCC30.24896265560165973No Hit
CTGCAGAGAGATACTAAGCAGCAAAATCTTGGTGTTGTGATGTACAGAAA20.16597510373443983No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTCTGCC20.16597510373443983No Hit
TCTGTGAGTAGCCATGTGTGTTGATCAGATACAGTTTTTCTGAGATCTTC20.16597510373443983No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTAAGCC20.16597510373443983No Hit
CTCTTGTACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGCC20.16597510373443983No Hit
GCTATGGTGGGCAAACTAAGCCGATTTTCCGGAAAAAGGCTAAAACTACA20.16597510373443983No Hit
ATCTGAAAGAGGTTATTCGGGAAAGAAAAGAAAGAGAAGAATGGGCAAAG20.16597510373443983No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAAGCTCGTATGCC20.16597510373443983No Hit
CCGGAACTGGTCGAATGAGGCACCTAAAAATTGTATACCGCAGATTCAGG20.16597510373443983No Hit
CCTTTATTGAAATGTTTTCCTTTGTGCTTAACTGGCTGGGCATTCCACAG20.16597510373443983No Hit
CCTCTGGGGGTCCTCTCTGAGTCAAATCCAGTGGTGGGTAATTGTACAAT20.16597510373443983No Hit
CTCCCTTGGGCTCTGATGAAAAATTGCTGACTGTAGCTTTGGAAGTTTAG20.16597510373443983No Hit
GACACCAGGAAGGTGAGATCTTCGACACAGAAAAAGAGAAATATGAGATT20.16597510373443983No Hit
GCTTTCAAGTGCCTTTCTGCAGTTTTTCAGGAGCGCAAGATAGATTTGGA20.16597510373443983No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCTCCGGCTCAACAA20.16597510373443983No Hit
CTCTTATACACATCTCCGAGCCGACGAGACGCCTCAATATCTCGTATGCC20.16597510373443983No Hit
CTCCTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGCC20.16597510373443983No Hit
CCCAGAGGAGCCACCAGTTCTCATGGGTGGCACTCAGTCTCTCTTCTCTC20.16597510373443983No Hit
GCCCCTGGGCCGCTAGAAGGGAGCCAAGCTGACTCCTGAGGAAGAAGAGA20.16597510373443983No Hit
CCCCACTGGGACGGAATAAATGCTCTGCAGACCTGGAAAAAAAAAAAAAA20.16597510373443983No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTGGTAATCTCGTATGCC20.16597510373443983No Hit
GCCATAACATCTTTTGCCACGTATAGCTGGAATTAAGTGTTGTCTTGGAG20.16597510373443983No Hit
GGCATTGGGGTTGGTGACTCTGATGCCAGGCTGCGCAGTGAAGAAAATGA20.16597510373443983No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC20.16597510373443983No Hit
CTCTTATACTCATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGCC20.16597510373443983No Hit
GCTGTAGATGAATGTTCTTAGCTGTCATGTTTAAAAATACTTCTGCTTCG20.16597510373443983No Hit
GATGGCAGCCGGCTCATAAAGGTTCATTTGGACAAAGCACAGCAGAACAA20.16597510373443983No Hit
CTCAGAACTGTTTGTTTCAATTGGCCATTTAAGTTTAGTAGTAAAAGACT20.16597510373443983No Hit
GTGAATGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.16597510373443983No Hit
GTTCAATTGGAGAATTTTAATGTTTTTCATTTATCATTGTAAAACCAAGG20.16597510373443983No Hit
TATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGCCGTCT20.16597510373443983RNA PCR Primer, Index 6 (95% over 21bp)
CCCATAGAGGGCTGCGTCACTTCTGGGGCCTTCGTGTCCGAGGCCAGCAC20.16597510373443983No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers