FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTTCGAATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG11.282051282051282No Hit
GGCTCGCTGGCGTGGGGCCGGGCGTGGACTGCGAGTGCCTAGTGGGCCTC11.282051282051282No Hit
GTCCTTGCCAACTCTTTTCCCTCAAAAGTTTAACATTTAATTGGAGAGGT11.282051282051282No Hit
GCAGGCATGGGAGTAGATGCTGAGAATTCTAGCCATAAGAAGAAAAGTAT11.282051282051282No Hit
CTTTTTTAGATGGGAATAAAATATCTCTGTGACAAGACAAAGATTAAATG11.282051282051282No Hit
GCGTGCGCCAGGGGCTGTTGTCGGCCGTCTCCTCCGTCCTGCTCAGCCTG11.282051282051282No Hit
GGGAAGAGGAGGGGTTTGAAAAGAGGTTGATTAATGGGTGTAAAAATATA11.282051282051282No Hit
GCTGCTGATGTGTTGTTGGCGAGGTGAGTCGGGTCGCGTCGGGGGGGGGG11.282051282051282No Hit
CCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGC11.282051282051282No Hit
GCTGCGGATATGGGTACGGCGCGGCGCGAGATGTACTCCCTCTCGCCCGG11.282051282051282No Hit
GCTATCACACAAGATGCTCAGCTAGAGAAAAATGGAATCTAAGAGCAATA11.282051282051282No Hit
GAAATACAAAGTGGATTTAACATTTGATAAAAAATCAATCAATGTAATTT11.282051282051282No Hit
GGCATAGGCAGTATCTAGATCCAGAGTAAAGTTGTCAGCTTGCTCTTGAC11.282051282051282No Hit
CCAAGGAGCAGGGAGTTCTGTCCTTCTGGCGCGGTATCCTGGCCAATGTC11.282051282051282No Hit
GCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCC11.282051282051282No Hit
GGCCGCTCGGGGGTCGCGTGACTAGATAGCATGCCAGAGTCTCGTTCGTT11.282051282051282No Hit
CCATAAGCAGGTGTGTGAGATCATCGAGAGCGCCCTCTTTCTGAAGTTGC11.282051282051282No Hit
CGGACATCTAAGGGCATCTCAGACCTGTGAGTGCTCAATCTCGGGTGGCT11.282051282051282No Hit
GGTGAATGGGGGGGGCAACTTGCCAACATTAGGTAAGGTGAATTAAGATG11.282051282051282No Hit
CCTTACAGCCGCTCGTCAGACTCCAGCAGCCAAGATGGTGCAGCAGCTCG11.282051282051282No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG11.282051282051282No Hit
GAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGG11.282051282051282No Hit
GTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC11.282051282051282No Hit
GCTGATGATGTGTTGTTGCCCTGGTAATCCTGCTCAGTCCGAGAGGAACC11.282051282051282No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCTTAGTCGCACAGG11.282051282051282No Hit
CTCACTCTGGAAGCACTCGGGCACCTGGGTAATGATGACTCAGCTGACTG11.282051282051282No Hit
CCAGTATAGTGTATATAATCACTGTGTTCTTGGTCACTACTGGAGAGATT11.282051282051282No Hit
CCCATTTGCTCGCAGTATCCTAGAATCTTTGTGCTCTCGCTGCAGTTCCC11.282051282051282No Hit
CCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGT11.282051282051282No Hit
GGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCTCCACC11.282051282051282No Hit
GACATCTAAGGGCATCTCAGACCTGTTATTGCTCAATCTCGGGTGGCTGC11.282051282051282No Hit
CCAGGCAGTAGGCAGTGACCTGACTGCAGAAGAGCAGAGGACAGACTCCG11.282051282051282No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA11.282051282051282No Hit
GCCAGTTTGGTCAGTCCTGTTGTTTGCGAGCTATAATTGACATGAGTAGT11.282051282051282No Hit
TCCCTCAGGATAGCTGGCGCTCTCGCTCACGCGACGCACCCCCGCCACGC11.282051282051282No Hit
CGTCATCTAATTAGTGACGCGCATGAATGGATGATCGAGATTCCCTCTGT11.282051282051282No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA11.282051282051282No Hit
CAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTT11.282051282051282No Hit
GCTCTCTTGTCTTCCGTGCAGGAATTCCCAGGTTCTCAGCTTGCTGGATA11.282051282051282No Hit
GCTCACGTTCCCTATTAGTGGGTGAACACTCCAGCGCTTGGTGAATTCTG11.282051282051282No Hit
TCCGTGGCTCGGTGTGTATAAGAGACAGTTTTTTTTTTGTTTTTTTTTTT11.282051282051282No Hit
GGAATCAGCGGGGAAAGAAGACGCTGTTGAGCTTGACTCTAGTCTGGCAC11.282051282051282No Hit
CCTATCATTGTGAAGCAGAATTCTCCAAGCGTTGGATTGTTCACGCACTA11.282051282051282No Hit
GCTGGAGCTGGCATGGGCGCGACGTCTGCCCAGAAAGCTCAGAAGGCTTA11.282051282051282No Hit
GGGACAGGCGGGGGACCGGCTCTCCGAGGCCAGCCGAGGCTCCGCGGCGC11.282051282051282No Hit
CCTATATGGCCTTCTTTTGGACAAACCTTGAAAATGTTTATTTAAAAAAA11.282051282051282No Hit
GGTGAGGCGGGGGGGCGAGCCGCGAGGCTCTCTCGGTGCGGGGGCGAGCC11.282051282051282No Hit
GCACTTTGGGAGGCCGAGGCAGGTGGATCACGAGTTCAGGAGATTGAGAC11.282051282051282No Hit
GGTACACAGTAAGGGTTAGAACTTTTATAATTTACACACAGTAGAAGGAT11.282051282051282No Hit
CCGCGGCGCTGCCGTCTCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGC11.282051282051282No Hit
CCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGA11.282051282051282No Hit
CCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGCCTCCGT11.282051282051282No Hit
GCCCGTGGACGGTGTGAGGCCGGGAGCGGCGCCCGGCGCGCGCGTCCCTG11.282051282051282No Hit
CCGCTTTCACGGTCTGTCTTCGTACTGATTATCTAGATCTAGCGCGCTTT11.282051282051282No Hit
GGCGGGGGCAGGGCCTGGAGTGTTGGGGGCAGCCTGGGCAGACGTCCGCT11.282051282051282No Hit
CCCGGTGGGGTGCTGTAGAAGCAGCCCTTTCCATCTACCTTGAAAACTAT11.282051282051282No Hit
CTTACAGACCCGAGTGGAATCTGTAGAATCTCAATCAAGACAACTTGAGC11.282051282051282No Hit
CCCTCTGGGTGGCTTTGTCCACTATGGTGAAGTGACCAATGACTTTGTCA11.282051282051282No Hit
GCTGGTGTCTGGGTTATAGGCCTGATGGGCCTGGTAGATTTCAATCTTGT11.282051282051282No Hit
TCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTC11.282051282051282No Hit
CAGGATAGCTGGCGCTCTCGCAGACGCGACGCTCCGCCGCCTCGCAGGTT11.282051282051282No Hit
GCGCCGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCGATGTTCAGGGC11.282051282051282No Hit
CCAGAACACAGAATTGTAAAGATGCTACTTTGTAGCTGTGTGAAACAACA11.282051282051282No Hit
CCTTTATCTGGCTGTGCAGCCTTGCTCTGTGGCTTTTCCCAGCTCTATCG11.282051282051282No Hit
GGTCTCAGGGTGGCTGGGTGAGCGCTCATCAGTCGCAGACAGCAGGGTGG11.282051282051282No Hit
GCTCACGTTCCCTATTAGTGGGTGATCACTCCATCGCTTGGTGAATTCTG11.282051282051282No Hit
GCATAATATAGCTAGGACTCACCCCTATACCTTCTGCATAATGAATGAAC11.282051282051282No Hit
CCAGGAGTGGAGCCTGCGGCTTAAGTTGACTCTACACGGGAAGCCTCACC11.282051282051282No Hit
GTGTTGGAGGCATAAATATTTTTACCATTAAACTGTTTATTCCCCTCCTA11.282051282051282No Hit
CCCATATGACAGAGGATCTGCTGTTGCTATTGGGTCAGATGGTCTTTGTT11.282051282051282No Hit
GCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGC11.282051282051282No Hit
CCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTT11.282051282051282No Hit
ACTTGATTTATATCTATAAAAAGACTGACAGGTAATTATATTTGGAAAAC11.282051282051282No Hit
CTTTATTGGCTGCTACATGTTATTGCAAGAGACTTTGCTGCCTTAAATGC11.282051282051282No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTGTTTGTTTTTGGTTGTTTTTGT11.282051282051282No Hit
CTCCTGCCTATCGTATAGAGCTGCAGAAAGAGTAAATGATACACGGTCAA11.282051282051282No Hit
CCTCAGTAGTTGGGATTACAGGAATGTGCCACCACGCCCTGCTAATTTTG11.282051282051282No Hit
CCCAAAGTGCTGGGGTGAAAGGTGTGAGCCACCGTGCCCAGCCTCCATAT11.282051282051282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAG50.044.029
GAGGCGT50.044.025
GCGGGAT50.044.09
CAGTCTT50.044.033
TGACTTA50.044.018
GGCGTTC50.044.027
CGCCTGG50.044.02
CTGGGCG50.044.05
GTCGCAC50.044.041
CCGCCTG50.044.01
AGAGGCG50.044.024
AGGCGTT50.044.026
TTCTGAC50.044.015
GGATTCT50.044.012
CTTAGTC50.044.037
CTGACTT50.044.017
GTTCAGT50.044.030
CTTAGAG50.044.021
TAGTCGC50.044.039
TCAGTCT50.044.032
Produced by FastQC (version 0.11.5)