FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATGCTGAAGGAGATGAGTGGGCTTCGGAGTGAATGTGGCAGTTTAAA11.639344262295082No Hit
CTTCCGCTGTCACGGTAAAGTCCGCCATCAGCAGACTGAAGGAGTAGAAA11.639344262295082No Hit
CCTACCTCAGAGGGGGACAGACAGAGAGGTGTCAGCATATTCACCTGTTT11.639344262295082No Hit
GCTTCAGCTCCTAGCTCAGCCACTGACTGCACCTCCTGAGTGTGCACATC11.639344262295082No Hit
GCGTATTGCTCTGAAGAAGCAGCGTACCAAGAAAAATAAAGAAGAGGCTG11.639344262295082No Hit
ACTCCCAGGGGCCTTTGCAGCGTCTTTTTAACCTCAGAAAATTTCTCAAT11.639344262295082No Hit
TCTGATGCATAGCAGTTAGTTATCACCTCCTGAGTCGATTTGTAATTTCC11.639344262295082No Hit
GCCCTGGGCAGCCAGCATTCATTGTAAGTTCCCTCTTTGAAAACTCGTGT11.639344262295082No Hit
GCTGAATGTAAAGATTGAACCTCAAGTCACTGTAGCTTTAGTAATTTCTT11.639344262295082No Hit
CAGAATATGGAGGCTCCAAAGTAGTTCTAGATGACAAGGAGTATTTCCTA11.639344262295082No Hit
GGGGGTTTTTTATTATAGGAATGTCGAGCATGAACTTGATACGTCCGTGT11.639344262295082No Hit
GCGAGGCCAGGCCGCTCTGGACCGTCTCAAGGTGTTTGACGGCATCCCAC11.639344262295082No Hit
CATAATTTGGAGGGAAGCTCTTGGAGCTGTGAGTTCTCCCTGTACAGTGT11.639344262295082No Hit
GCTCCTCAGGCGGCGGCGGCAGGAGACTCGCTGGCCCCCCGGCGGCAGCG11.639344262295082No Hit
CTTATGCTCGGCACCAACAGGTCCGACAAATCCGGAAGAAGATGATGGAA11.639344262295082No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTGTGGTTTTCTTTTTTGCG11.639344262295082No Hit
GCGCAAGGTGGCCTGTATTGGGGCATGGCATCCTGCTCGTGTAGCCTTCT11.639344262295082No Hit
AACTCTGGGAGGCTGAGGTGGCTGGATCGCTTGAGTCCAGGACTTCGAGC11.639344262295082No Hit
GCACTTTGGGAGTCCAAGTCAGGCAGATCAACAGGTAAAGAGACCGAGTC11.639344262295082No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC11.639344262295082No Hit
GCTACAGGGGAGGCTGTGGCAGGAGGATCGTTTGAGCTCAGAAGTTTGAA11.639344262295082No Hit
GCCATTGTGCCTGGCAGACTAATTTCAATAAGAACACATGCCATGGTGGA11.639344262295082No Hit
CCTGCAGAGACAAATCTTCTGCATGAGATTACTGATGCTTCACTTGCACT11.639344262295082No Hit
GCACAACCTTATCATTTTTAAATAAAAACAGGGAAATGGTTTAACAAACT11.639344262295082No Hit
GGCCATGCGCTGGTTCCAGCAGAAGTATGATGGGATCATCCTTCCTGGCA11.639344262295082No Hit
GTCCTAGGGAGAATCACAGGCCTGGCAGACAAGACCACACTGAGAGCAGT11.639344262295082No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTATGCCGT11.639344262295082No Hit
GCTGTCCGGGAGGCTGAGGCAGGAGGATCTCTTGCACCTGGGAGGAGGAG11.639344262295082No Hit
GCTCGCTGAGGCGGACGTGTCGAGGCTCGTCGACGCTCCCCCTCCTCCGG11.639344262295082No Hit
GTGTGCAAGGGGGTGGCCATGGCTACATTAGTTGCCTCTGCTTCTGATAT11.639344262295082No Hit
TCTCTGTGGTCTTGTTTAAAAAGGGCAAAAGTATTAGACTGTACTAAATT11.639344262295082No Hit
CCTACTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGTGGAG11.639344262295082No Hit
ACAGACTTATTTCTTCTTGGACACACGCACGGTGCAGCCACGAAGAAATA11.639344262295082No Hit
CCTTGTGGAGTATATAGATGCTTTTCATTATACACACAAAAATCCCTGAG11.639344262295082No Hit
CCAGTGGATTTCCTCTAGTTGGTCTAAGAAATCTGAAGAGGAAAAACAGA11.639344262295082No Hit
CTTATTTTACTTCTTTTAGCTGTTTAACTTTGTAAGATGCAAAGAGGTTG11.639344262295082No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATTCCATCTCGAATGC11.639344262295082No Hit
CACAAAGTGCTGGCCCAGCCGAGATTTGTTTTCTAAGATACTTAGTGTCG11.639344262295082No Hit
CCCTTTACCAGGGAAATCACTTCCTTTAGGACTGAAAGCTAGGCGTCCTC11.639344262295082No Hit
GGGGGGGGGGCAGTTCATACTTTAGACTGGAGATAATAAGTAAGCAAGTG11.639344262295082No Hit
CCCTCATAGAACCTGTTGCATGCAACTTAGTTTCACAGCTTTGCCTCTTC11.639344262295082No Hit
GGGAGGTAGAGGTTGCAGTGAGCCGAGATCACGCCACTGCACTCCAGCCG11.639344262295082No Hit
GCAGTGGTATCAACGCAGAGTGAATGGGAGCCAAGATTGCACCACTGCAT11.639344262295082No Hit
GGCAATATGCTGGGCACCTACTTTAAAACAAGGTTGTCTGATTTTTGCAA11.639344262295082No Hit
ACACGGGTGTGGGGGAGAGATTGAGACTGAGATACAACTCAATCCAACTC11.639344262295082No Hit
GAGAAGGGGGAGGGAGAATTTATTAATAAAAGAATCTTTAACTTTAAAAA11.639344262295082No Hit
GCTATGTGGGAGGATTTAGATTTAATGGTGATTAATAACAAATATGTAAG11.639344262295082No Hit
GAGTGAATGGGGGGGTGAAAAAACAAAAAAAAAAAAAAAAACAGAGAATT11.639344262295082No Hit
TTCTTATACACATCTCCGAGCCCACGAGACAAGAACTGATCTCGTATGAC11.639344262295082No Hit
GCACTTTGGAGGGCCGAGGCGGGCAGATCACTTGAGTTTAGGAATTTGAG11.639344262295082No Hit
CCCAGGGGTACATTTTTGCACTGAAATCTAAAGATGTTATAAAAATCACT11.639344262295082No Hit
GTGTATGGAGAGATTCAAGAAATAATTTTAAAACCGCATACATAATAAAA11.639344262295082No Hit
CCCCTGGTGGGGGATTGCACTGGCAACATTTGGGTCTCAGCAAAAAAACC11.639344262295082No Hit
GCCGGCCGGCTGTCAAGCAGTTCCACGACTCCAAGATCAAGATCCCGCTC11.639344262295082No Hit
CCCAGAGGGGAGGCTCTGGGACAGGGTCTGTTGTTCAGTTCCATGTCACT11.639344262295082No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA11.639344262295082No Hit
GAGTGAATGGGGCTTTAATTGCCATGATCATTCCCTCTTTTTGGATGTAT11.639344262295082No Hit
ACCTAGATTTCTCATGACGAGTTAATGCATGTCCGTGGTTGGGTGCACCT11.639344262295082No Hit
GCGACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAAAAAAAAAAAACGAG11.639344262295082No Hit
TACCCATAAAATTGTTTGAAAACTTTCACCAGAAAGGGATGTAAGTCTCG11.639344262295082No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC11.639344262295082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCGG50.044.08
CGCTGAG50.044.04
TCCTCCG50.044.043
GTGTCGA50.044.017
CTGAGGC50.044.06
ACGCTCC50.044.033
TCGACGC50.044.030
CTCCCCC50.044.036
CTCCTCC50.044.042
CGCTCCC50.044.034
GTCGAGG50.044.019
GTCGACG50.044.029
AGGCGGA50.044.09
CGGACGT50.044.012
GCGGACG50.044.011
CGAGGCT50.044.021
CCTCCTC50.044.041
CCTCCGG50.044.044
GACGCTC50.044.032
GCTCGTC50.044.025
Produced by FastQC (version 0.11.5)