FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGGACGAGACAGGTGCTAACGTTGAACGAGCTGATGGATATGAACCA12.0No Hit
GGAGAGACCAGCCTTTCTTCCTATGGTAGGAATGGCATGAGTTGGCGTTG12.0No Hit
TCCCTACAAGACCTACTCCATAAAATTGGAAGAGGTAACTTTCTCATGAG12.0No Hit
GCACTTTATTAGTGGGGAAACTCGCCTTGGTCAGGCAGAGACAGGGAGAA12.0No Hit
CTTTTAGATGGTTTTTTTTAAAAAAAAAAAAAAACTGGTAAAATAACTTT12.0No Hit
GCACTTTGAGAGGCCGAGGCAGGTGGATCACCTGAGGTCAGGAGTTTGAG12.0No Hit
AATATAAAGGAGAAAGTCACGTGCTTCAATCAGTGCTGCATCATAGAATA12.0No Hit
GCAAGAGGGGAAGCTGACAGAGCTACCTACAGACGGAGTGCTGTGCCACC12.0No Hit
GGGAGGGGAGGCAGATGGAGCCCATAGGCCACCCCCTATCCTCTGAGTGT12.0No Hit
AGTGATGGGCTTGTTTTTGTAAAATTACCAGAAAACTCAGTGGAGATTTA12.0No Hit
GGTGAAGGGGGACCTGGTACCCAGTGATCCCCACCCCTGGATCCTAAATC12.0No Hit
TCCCTGAGGTTTGTGTATGTTGGATATTGTGGTGTATTAGATCACTGAGT12.0No Hit
CCGCCTCCGCGCGACTCGGCGGCGGCAGGCGGAGGCTTGGGTGCGTTCAG12.0No Hit
GCTACTCGGGAGGCTGAGGCAGGAGAATCACTTGAAACTTGGAGGCGGAG12.0No Hit
CCCAAAGCTGGTTTCAAGCCAACCCCATGGCCTCCATGACTTTTCCAAAA12.0No Hit
GAAAAAAACTCCATGTCAAGAAAGTGGGATAAGAAAAATAGTGAAGTATT12.0No Hit
ATTTGGGTAAAGAAACCATTTGCTAAAATGGAGAAAGTAAATAGATTTTT12.0No Hit
GCCTAAAAGTGGGCTTGATTCTGCAGTAAATCTTTTACAACTGCATCGAC12.0No Hit
CCTATTAAGAAAAATGTGATGCCCTATCAATGCAACTTTTCAGATTTAGT12.0No Hit
CAGACACAGAAAGGTCACCTACATCTCATACTTGCTTTAATGTGCTTTTA12.0No Hit
GGGGCCTGGGGGCAGTTTGAGCCTCCTCTCCCTTCTGTGGGTCGCTCCCA12.0No Hit
GGGAGGCGGAGGTTGCAGTGAGCCAAGATTGCACCACTGCACTCGAGCCT12.0No Hit
CTTATAACACTCCGAGCCCACGAGACGAACGGTAATCTCGTATGGCGTCT12.0No Hit
GCCCGACGGGAGTCCAAGGTCAAGTTTGAAGAGAGATACAAGACAGGCAA12.0No Hit
GCATATGAAGTAAAAAGGAATACTGTGAAAGGGGAGTACTCTTGTACAGC12.0No Hit
GCTACTCGGGAGGCTAAGGCCGGAGGATCACTTGAGCCCAGGAGGCAGAA12.0No Hit
GCAATTGTTGGGATGTGGATGGATGTGAGCATAAGAGAGAGAGAGAATAT12.0No Hit
GAGCCAGACTACTTAAGTACTTTCTTTACAATTAAAATGGATATTATAGC12.0No Hit
GCCTAAGAGGTTGCAGTGAGCTGAGATTGTGCCACTGCACTCCAGCCTGG12.0No Hit
GGGCAACAGAGTGAGAGAGACTCTGCCGCAAAAAAAAAAAAAAAAAAAAA12.0No Hit
GCCAAGAAGGGCCTTACTCCTTCACAGATCGGTGTAATCCTGGGAGATTC12.0No Hit
GGTTTAGGTTATGTACGTAGTCTAGGCCATATCTCTTGGAGATTGAGACT12.0No Hit
GATTTTTGTGGTTAGTACTGCCCTTGCCATGGGTCACACTATGATGCATC12.0No Hit
CTGAATCTAAACGAATAACTAGAAGACAATTGTTATAATTTTTATAGGCT12.0No Hit
GGGTGGTGGTGGGTTCCTTTAATCCCAACTCCTGGGGCGCGAGAATCACT12.0No Hit
TCCTGGGATGGGGCCTTCCAGCATGTGGGCAAAGACTTCAATCAGGGCAA12.0No Hit
GCCAATAGGCAGCTTTCTTAACTCTCCTAACAAGCCTTGGACCAAATCGA12.0No Hit
GCACAGGAGGCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.0No Hit
ACAGGTCATGCAAGAGCACCTTCATCATCGTCTTTAAACAGCGCAACAAG12.0No Hit
GCTTTTGTTTCCTTTTCCAGATATCTTGGACAAATCACATATTTTAAAAT12.0No Hit
GGCTGGGAGGCCAGAGATGCTAATTTCCCTACAATGTATCAGTGAAAGGA12.0No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTGGGTTTGTTTTTTTT12.0No Hit
GGCTGATGCAGGATTTGTTCTCTGTCCAGCAGTCACTTCGGCCAGAGCTG12.0No Hit
GCTTGATCCCCCTCTTTGTATTTATTGGAACTGGAGCTACTGGAGCAACA12.0No Hit
GTAAGTGGAGTATTTTAGTCTAAAGGCTTTTCAAATTACTTGAATTTTTT12.0No Hit
AGAAATTAAGAGGGAAGATGATTAAGAGAGATGATTATTATTATTACACT12.0No Hit
GAAGGCGGAGCTTGCAGTGAGCCAAGCTCGCGCCACTGCACTCCAGCCTG12.0No Hit
GCTAAAATCAAGTCATTGCATTGTGTTCTAAATACAAGTATGTTGTATTT12.0No Hit
CATATGGAGTGATTGTTTCAGTCCAGGCAATTTTTCTATTTTATATTAAG12.0No Hit
GCTCAAGTGAAGGCTCCACTTGTTCTTAAAGACTAGAGCAGCGAACTGCG12.0No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGCC50.044.021
GGGCAAC50.044.01
ACAGAGT50.044.06
GAGTGAG50.044.09
TGCCGCA50.044.024
GGCAACA50.044.02
ACTCTGC50.044.020
CAACAGA50.044.04
CCGCAAA50.044.026
CAGAGTG50.044.07
GCAACAG50.044.03
GTGAGAG50.044.011
TCTGCCG50.044.022
AGAGTGA50.044.08
GCAAAAA50.044.028
AGAGAGA50.044.014
AGAGACT50.044.016
AGTGAGA50.044.010
CTGCCGC50.044.023
GAGAGAG50.044.013
Produced by FastQC (version 0.11.5)