FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40133
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCC16414.088904392893629No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGCTCTCGTATGCC7791.9410460219769265No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCCGT2500.6229287618667929No Hit
CTCTTATACACATCTCCGAGCCCTCGAGACCAGAACTGATCTCGTATGCC2450.6104701866294571No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2310.5755861759649167No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGATCTGATCTCGTATGCC1700.4235915580694192No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTCTGCC1350.33638153140806815No Hit
CTCTTATACACATCTCCGAGCCCTCGAGACCAGAACTGCTCTCGTATGCC1310.32641467121819945No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC1220.3039892357909949No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCCG1140.28405551541125756No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGATCTGCTCTCGTATGCC910.2267460693195126No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGC890.22176263922457826No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGAGCTCGTATGCC890.22176263922457826No Hit
GCAGGAAGGGGGGTCGCCGTGGTCGCCGTCTGTGAACAAGATTCCTCAAA870.2167792091296439No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAACTGCTCTCGTATGCCGT740.1843869135125707No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGCTCTCGTCTGCC730.18189519846510352No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCACTGATCTCGTATGCC660.16445319313283333No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCACTGCTCTCGTATGCC650.16196147808536615No Hit
TCTATTGATGAGGGTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.16196147808536615No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGAACTGATCTCGTATGCC580.14451947275309596No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG530.13206089751576008No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA510.12707746742082576No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGCTCTCGTATGC430.10714374704108838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTT302.466917E-644.00000412
GTCTGCC502.5465852E-1144.044
GAACCCG207.7658275E-444.034
GAGGGTC351.3998942E-744.010
TCTCGTC557.2759576E-1140.040
TCTCTTA850.038.823531
AGGGTCT403.993573E-738.511
TCTATTG403.993573E-738.51
TGAGGGT403.993573E-738.59
ATGAGGG403.993573E-738.58
CTCTTAT5550.038.0540541
GCACTTT357.1160193E-637.7142871
CTCGTCT651.0913936E-1137.2307741
TCTTATA5700.036.6666682
CGTCTGC601.8553692E-1036.66666843
GTATGCC5250.036.4571444
ATGCCGT553.252353E-936.044
ACTCGGG250.002325856435.24
GATCACT250.002325856435.226
CTCGGGA250.002325856435.25
Produced by FastQC (version 0.11.5)