FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10762
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGCC910.8455677383385988No Hit
GCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGACCAAATGGA600.5575171901133619No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG430.3995539862479093No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC300.27875859505668094No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATCTCTCGTATGCC280.2601746887195689No Hit
CTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAA190.17654711020256458No Hit
CTCTTATACACATCTCCGAGCCCTCGAGACGCCTCAATATCTCGTATGCC160.1486712506968965No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG110.10221148485411632No Hit
GCAGGAAGGGGGGTCGCCGTGGTCGCCGTCTGTGAACAAGATTCCTCAAA110.10221148485411632No Hit
GCAAAGAGGAGGCCATGCGCTGGTTCCAGCAGAAGTATGATGGGATCATC110.10221148485411632No Hit
CCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGA110.10221148485411632No Hit
GCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAGGCTAA110.10221148485411632No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAC207.5132103E-444.036
GAGTGAA207.5132103E-444.01
CACTCCA207.5132103E-444.040
AGTGAAT207.5132103E-444.02
GCACTCC207.5132103E-444.039
CAGTGAG254.154708E-544.016
AGTGAGC301.2173562E-436.66666417
GTTGCAG250.00225090735.212
GAATGGG401.6588676E-533.05
TGCACTC353.0122476E-431.42857238
TGAATGG353.0122476E-431.4285724
ATCTCCG300.00549856629.33333212
CATCTCC300.00549856629.33333211
GGAGGTT300.00549856629.3333328
TCCGAGC300.00549856629.33333215
CTGCACT300.00549856629.33333237
CTCCGAG300.00549856629.33333214
AATGGGG406.5862347E-427.56
GTGAATG406.5862347E-427.53
Produced by FastQC (version 0.11.5)