FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATGGGTACAATTTTTAATAAAAACTTTATTTTGTTCCTTAAAAAAAA110.0No Hit
GTGCGAGAGGAACAATTCCCACGCTGGGGAAGGACTTGTCTCCTATTCTG110.0No Hit
GCTACTCGGGAGGCTGAGGCAGGAGAATGGCATGAACCCGGGAGGCGGAG110.0No Hit
GCTGTACATACTTTTTGGGTTTATTTGGAAAGGAATGCAAAGATGAAGGT110.0No Hit
CCCACTTTGCTGTTCCTTACTTTACTGCCTGAATAAAGAGCCCTAAGTTT110.0No Hit
GCTACTAGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGAGAGAGAGAT110.0No Hit
CCTTTACCCTGCCCTTGTTGAACATTTATATAATCTAACCTGGACATCAA110.0No Hit
GGGTGGAGGGGGTGGAGAGGGAGGATGGAGATAGCAGCGACGATGACACT110.0No Hit
GGGGATGCTGGTACAAGTTGTGGGACTGCATGCTACTGTCTAGAGCTTGT110.0No Hit
AAATACATGGAAGCTAAATAACACACTTATATACAACTCATGGGCCAAAG110.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)