FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTATGCC927.27272727272727No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCCGCTCGTCTGCC13.0303030303030303No Hit
CTCTTATACACATCTCCGAGCCCTCGAGACTTCCATCCATCTCGTATGCC13.0303030303030303No Hit
GTCTTATTCAGATCTGCGAGGCGACGTGACTTCGATCGAGGGCGTATGCC13.0303030303030303No Hit
GGGCTGATTATTATACTTCTCTTAATAGCTTATTTCTTGCTAGTGCTATT13.0303030303030303No Hit
GATGTGTATATGAGACAGTTTTTTGTTTTTTGCGGGTAAGGCGGGAGGGG13.0303030303030303No Hit
GGTGGTAGCTGGTTGTCCAAGATAGCATCTTAGTTCATCTTTAAAGTTGG13.0303030303030303No Hit
GCTACTGGGGAGGCTGAGGCAGGAGAATGGTGTGAACGCGGGAGGCAGAG13.0303030303030303No Hit
GCCATTCAGCCGCTGCTGGTCGCTTGTGGGTCCTGCTGCGGGGATTCGCG13.0303030303030303No Hit
CTCTTATAACACTCCGAGCCCACGAGACACGTTCGAATCTCGGAAGACGT13.0303030303030303No Hit
GGCGGGGGCAGAGATTGCAGTGAGCCGAGATTGCGCCACTGCACTCCAGT13.0303030303030303No Hit
ATTCTCTCTTCTATAAAAATGATTGGCGTATCCAACCTGCAGAGTGTTAT13.0303030303030303No Hit
CTCTTATACACATCTCCGAGCGCACGAGACTTCCATCCATCTCGTATGCC13.0303030303030303No Hit
TCTCTTATACTCATCTCCGAGCCCTCGAGACTTCCATCGCTCTCGTATGC13.0303030303030303No Hit
TGTTTTATTCTGAGATTCTGGGTGTAGTATTTCTAATTTGATTGTGAGAT13.0303030303030303No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCGATCTCGTATGGC13.0303030303030303No Hit
CCGGGTGGGGAGGTGGCCAAGGATGCTGGAGCTGAATCAGACGCTGACAG13.0303030303030303No Hit
GGGTGTATGTGGGATGGTTTAGGCTGTGTTTTCAGAGGGTGCGGTTATGT13.0303030303030303No Hit
CTCTTGTACACATCTCCGAGCCCACGAGACACGTTAGCATCTCGTATGCC13.0303030303030303RNA PCR Primer, Index 24 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTCCCTCGCTCTCGTATGCC13.0303030303030303No Hit
CTTATACACATCTCCGAGCCCTCGAGACTTCCATCCCTCTCGTATGCGGT13.0303030303030303No Hit
CTTCTAGGGGCTGCATCACCATCATAGGTGGTGGAGACACTGCCACTTGC13.0303030303030303No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCCTCTCGTATGCC13.0303030303030303No Hit
CTTATACACATCTCCGAGCCCTCGAGACTTCCATCCATCTCGTATGGCGT13.0303030303030303No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGCAAGATCTCGTATGCCG13.0303030303030303No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)