FastQCFastQC Report
Wed 28 Aug 2019
H2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY7BGXC_n01_Lu_RNAseq_Aug2019_Sample_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGTGCCCCTGCTGTGTGGCACTGTATGACTGTTGCTGAAATATA11.1904761904761905No Hit
GCCACGCTGGCTGGCTTCCTGGACCTCACAGAGCAGGAGTTCCGGATCAA11.1904761904761905No Hit
GCTGTTGCCAGTTCACCAGAAGATCACTATTAACGATAATAGCTCTATCT11.1904761904761905No Hit
AGTGAGACCTGGTCTCTAAAAAATAAAATAAAATAAAACAAACAGGTCAA11.1904761904761905No Hit
CTTGTTTTAGGCCTCGTTTACTTTTAAAAAATGAAATTGATCATAGCTGG11.1904761904761905No Hit
GCCTTCGGAGCGTTCTCTGTCCTACTTCTGACTTTACTTGTGGTGTGACC11.1904761904761905No Hit
GGTTTAGGACCTATTAAAAATTCAAACAACTTTCTTTTTATTATTATTTT11.1904761904761905No Hit
GCTAGCGCCTCATGTTTTACATTTTGGCACTAACTGTCATCCAGATACTC11.1904761904761905No Hit
GACATCTATGTTATATGTACTCATGGATAAATATTTCACAATAAAACTAT11.1904761904761905No Hit
ACTACTTGGGAGGCTGAGGCAGGAGAATGGCGTGAACCAGGGAGGCAGAG11.1904761904761905No Hit
CCACTTCTCCAACTTCTGTTTAATCTGATCACGGCTCTTTTTAAGCCCTG11.1904761904761905No Hit
GTCCTCTGACTAGCTATCTAATTTCTAAGAGGAATATCCTATCAATCAGC11.1904761904761905No Hit
TATCTGAAAGAGGTTATTCGGGAAAGAAAAGAAAGAGAAGAATGGGCAAA11.1904761904761905No Hit
GAGTGAATGGGCATTGTGCACATGTATCCCATAACTTAAAGCTAAAAAAA11.1904761904761905No Hit
CACTAAGGTGCGTCATGTCCCGGGCCGCTGAGTCTGTGGTGACCAGTGAA11.1904761904761905No Hit
CCTCTACTCTTTGAGACATCACTGGCCTATAATAAATGGGTTAATTTATG11.1904761904761905No Hit
TCTACAACACCTCCATTGAGTAAATGCCACTCAATCATGCTGACATCATA11.1904761904761905No Hit
GTTGGTCCTCTCCGAGAGGAAGAGTCACTGATGTCTGTCGGATGGTCTCG11.1904761904761905No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGG11.1904761904761905No Hit
GCCAGGTCTGGGTGAAAGGGATACTTTTATGCAACCATAAGACAAACTCC11.1904761904761905No Hit
GATGAATGGGAGTATTCTGTACATGAATCATGCTGTATTTTAAATCAGGA11.1904761904761905No Hit
GAACCTGGGAGGTGGAGGTTGCAGTAAGCCAAGACTGTACCACTGCACTC11.1904761904761905No Hit
GCAGATGGAGAGGCTTGAAAGAGAAAATGCCGCAGTGGATGGAGATGATG11.1904761904761905No Hit
GCTACTCGGGAGGCTGAGACAGGAGAATGGTGTGAATCCGGGAGCAGAGG11.1904761904761905No Hit
GGTGTTGATGTGGTTTTACGTCGGAGAGATTATAGGCAAGCGGGGCATCA11.1904761904761905No Hit
ACGATGGCCTCTTTTTGAGGAGTTATGCATAGACCCACTCTAGAGTAATG11.1904761904761905No Hit
GTTCCAGGGAGGGTCAGGCTCCAGCTGCAGCTCTATCTTCACTATGAGCT11.1904761904761905No Hit
CTATGACTATTGTTTTCAAAAAATTAATATATAAATAATTCAGAGAGGAT11.1904761904761905No Hit
CCCAAGAACAGGGTTTAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAAAA11.1904761904761905No Hit
CTGTGCCTGCGGGGGGGGGCCGTGGCAGAGCAGTCACTGGGGGGAAGGGG11.1904761904761905No Hit
TCTCTATACACATCTCCGAGCCGACGAGACTTCCATCCATCTCGTATGCC11.1904761904761905No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.1904761904761905No Hit
GCGTTGGGGGCAGGCTACTGGTGGGTATGCTGGATTAGTAGCTCCACCCA11.1904761904761905No Hit
GCTTCGGGGATATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1904761904761905No Hit
GCACTTTGGGAGGCCCAGGAGGGAGGATCACTTGAGGTCAGGAGTTAGAG11.1904761904761905No Hit
CTCGGGACGAGACAGGTGCTAAAGTTGAACGAGCTGATGGATATGAACCA11.1904761904761905No Hit
GCTCTTGGCATCGACTGTTTGCACAAGGGGACAAAAGATATGAAGCAACA11.1904761904761905No Hit
GCCAAGACTGGGGCACTGCTCTCCAGCCTGGCTAACAAAGCCAGGTGCTG11.1904761904761905No Hit
GGTTATTTTCAGAATGCATTCATTTCTGACTTTGGAAAAGTGGTTAAAAA11.1904761904761905No Hit
CCATTTGGGGTCCGCTTTTAACTTGGACTAGTGTAACTCCTCCATGCAAT11.1904761904761905No Hit
GACTGATTGGAGGGCCCTATCTTGTGAGTGGGGCATCTGTTGGACTTTCC11.1904761904761905No Hit
CCTTGATAGTGGGTCAGTCATCATTTATAAGTGCATTTCTAAAAATATTG11.1904761904761905No Hit
GCCGTGGCCGCACCGTGGGTGTGTCCAAGAAGAAATAAGTCTGTAGGCCT11.1904761904761905No Hit
CACAAGTGCTCTAAGATAAAGGTGATGGAAGAGAGCACAACATCACAAAT11.1904761904761905No Hit
GTCTATTATCTGCGATAGCAGCTGGAGAAAAATTCAAGATAAAGGTGAAA11.1904761904761905No Hit
GCTACTTGGGAGGCTGAGGTAGAAGGATCACTACTTAAGTGTGGGAGGGT11.1904761904761905No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCAGCTCGTATGCC11.1904761904761905No Hit
GCTGTTTCTTGTCATTGCTCTCTGGCTGCTTCCCCCCTGAGGTTGTGTAT11.1904761904761905No Hit
GCTTTGAGGAAATCTGAAATAGAGTACTATGCTATGTTGGCTAAAACTGG11.1904761904761905No Hit
TGCTTCAGGGACGTCCAGATCGGTGACATCGTCACAGTGGGCGAGTGCCG11.1904761904761905No Hit
CCGAGCCCACGAGACGAACTGTAATCTCTTCTGACGTCTTCTGCTTGAAA11.1904761904761905No Hit
GTGCCCAGGAGGTCGAGGCTGCAGTGAGCCATGATCATACCACTGCACTC11.1904761904761905No Hit
CCAGCCCCGGCCTTGCTTTGTCTGGCCTCACGTGTCTCAGATTTTCTAAG11.1904761904761905No Hit
GCGAGGGTGGGAGAGAGCGAGAGCGAGAAAGAAAGAAAGAAAGAAAGAAA11.1904761904761905No Hit
GCTACGTGGGAGGCTGAGTGAGGAGAATGCTATGAGCCTGTGAGGTAGAG11.1904761904761905No Hit
CCCGCAAGTCTAGAGCCAAAACTGTAGACCTTGATCCCGGCACTCTCTAT11.1904761904761905No Hit
GCCTAAAAGTGGGCTTGATTCTGCAGTACATCTATAACAACTGCCTCGAC11.1904761904761905No Hit
GCAGTACCAGTGGATCATTCTTGATTTAGTATTCATTAGAGTCATTTCTT11.1904761904761905No Hit
GCAACTCGGGAGGCTGAGGCAGGAGAATCACATGAACCAGGAAGGCAGAG11.1904761904761905No Hit
GGGAGAGGAGGAATAAACAGAGGAAATATTAATTAAATGGTCTGTAAACA11.1904761904761905No Hit
GGAAATGCTGAAGCTTTTGGACTTCGGCAGTCTGTCCAACCTTCAGGTCA11.1904761904761905No Hit
CATGAAGAGACGAGCTGTGGGGATCTGGCACTGTGGTTCCTGCATGAAGA11.1904761904761905No Hit
CCTATCAGGAGTTCACTGACTACCTCGTCAAGACACACACCAGAGTCTCC11.1904761904761905No Hit
GCTGCCAGGAGCTGTTTATTTAAAAACAGTACTTTCTTCTCTGGCTGAGT11.1904761904761905No Hit
GCCGGGTGTGGTGGCTCATGCCTGTAATCCTAGCACTATGAGAGGACGGG11.1904761904761905No Hit
ACAGGAGTTCTGGCCTGTGACTAACTAACTGCCCCACCCCAGCCGAGACC11.1904761904761905No Hit
GCTACCCAGGAGGGTTGCTGAAGCACATGTGCCTAATTTCATTTTTGATG11.1904761904761905No Hit
CCTCTTCTGGTTTAGGAACAATCTGTTCCTTTTCCGTAAGGATCATCTCA11.1904761904761905No Hit
GGCTGCTCGCTGGGTCTTGGATGTCGGGTTCGACCACTTGGCCGATGGGA11.1904761904761905No Hit
CTCCCTCCAGCCAATAGGCAGCTTTCTTAACTATCCTAACAAGCCTTGGA11.1904761904761905No Hit
GCTACTCAGCAGGCTGAGGCATGAGAATCGTTTAAATCTCAGAGGCTGAC11.1904761904761905No Hit
GCCTTGAGTGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1904761904761905No Hit
AGGAGGTGGAGGCTGCAGTGAGCCAAGCATGCACCACTGCACTCCAGCCT11.1904761904761905No Hit
CCTTGGAGGAGATAAACCAATTTTATGTCTATCATGTTATACAAAAATCT11.1904761904761905No Hit
GAGTTAATGGGGGGGGAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAA11.1904761904761905No Hit
CCCTGCCTGCCTCCCCAACACCGTGGAATCCTCACTGGTGTGCTGTCCAC11.1904761904761905No Hit
CCTAGGAAAGCTGTAGAGGAACCCCTTAATGCATCCAAAGAATCAAAAGG11.1904761904761905No Hit
CCTGCTGAGCCTCTATTTTCTATCTTTGATGATATGAATCAGTAATCTTC11.1904761904761905No Hit
CCCCATTCCTGGGAGGGGGCATCCTACGCCATATCTGAGGTGCGACTGCG11.1904761904761905No Hit
CCACCACAAACAACAACAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAA11.1904761904761905No Hit
GCCTTGTGGAGTATATAGATGCTATTCATTATACACACAAAAATCCCTGA11.1904761904761905No Hit
GCGGTCCCAGCGACTGTTTACTGAGAGCCAGAAACCCACGTCTCCACCTC11.1904761904761905No Hit
CCTTGTGGTGAAGGTTCTAAGACGTGGGATCGTTTCCAGATGAGAATTCA11.1904761904761905No Hit
GTGAATGGGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAGAGGGGGTTAG11.1904761904761905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAATG50.044.023
GGAGAAT50.044.022
CTGAGGC50.044.014
ACTTGGG50.044.04
GGCGTGA50.044.029
GCAGGAG50.044.019
ATGGCGT50.044.027
GGCTGAG50.044.012
GAATGGC50.044.025
AGAATGG50.044.024
AGGGAGG50.044.039
GCGTGAA50.044.030
CTTGGGA50.044.05
AACCAGG50.044.035
TTGGGAG50.044.06
CAGGGAG50.044.038
GAACCAG50.044.034
AGGCTGA50.044.011
AATGGCG50.044.026
GCTGAGG50.044.013
Produced by FastQC (version 0.11.5)