FastQCFastQC Report
Tue 3 Aug 2021
H2HY5AFX3_n02_SOR_R_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY5AFX3_n02_SOR_R_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12291156
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT289470.23551080142502462No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT247060.20100631706244718No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC204040.16600554089460748No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA196640.15998495178158995No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT195930.15940730066398962No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT177340.14428260450034153No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACA174460.14193945630500499No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT173940.141516387880847No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTA171650.1396532596283051No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGC167250.1360734498854298No Hit
AATAAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTC164330.13369775796515804No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC161630.13150105653203004No Hit
ATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG159270.12958097676085148No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT158400.12887315074351022No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG157330.12800260610149283No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT152360.1239590482782905No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA150870.12274679452445318No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA149500.12163217194542156No Hit
CAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTA149240.12142063773334258No Hit
GTATTTATTAGATAAAAAATCAATGTCTTCGGACTCTTTGATGATTCATA145400.1182964401395605No Hit
GTATTTACATTGTACTCATTCCAATTACAAGACCCGAATGGGCCCTGTAT143820.11701096300461894No Hit
GATTAATAGGGACGGTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAA139250.11329284243076893No Hit
ATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGCCA138930.11303249263128709No Hit
TGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACC138790.11291858959401378No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA138490.11267451165699956No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGAAGCTCG137520.11188532632732022No Hit
GTTTATGGTTAAGACTACGACGGTATCTGATCATCTTCGATCCCCTAACT137180.11160870466537078No Hit
GCTTAATCTTTGAGACAAGCATATGACTACTGGCGATGGTTCATTCAAAT137080.1115273453530327No Hit
ATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATT136540.11108800506640709No Hit
CTCCGGAATCGAACCCTTATTCCCCGTTACCCGTTGAAACCATGGTAGGC135380.11014423704328544No Hit
TCATCAAAGAGTCCGAAGACATTGATTTTTTATCTAATAAATACATCTCT134620.10952590626951605No Hit
CTCGTAAGGTGCCGAGTGGGTCATTAAAAAAACACCACCCGATCCCTAGT134110.10911097377659189No Hit
GTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTATC132420.10773600139807843No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT132120.1074919234610642No Hit
GTCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAAT131830.10725598145528378No Hit
GCACAAGGCCATGCGATTCGAAAAGTTATTATGAATCATCAAAGAGTCCG130500.10617390260118739No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAG129780.10558811555235324No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC127360.10361922019377186No Hit
AATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTAT122990.10006381824459798No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)