FastQCFastQC Report
Tue 3 Aug 2021
H2HY5AFX3_n02_SC_R_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY5AFX3_n02_SC_R_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8607138
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTA413650.48058948282228076No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT316140.36729979233515253No Hit
GCTTAATCTTTGAGACAAGCATATGACTACTGGCGATGGTTCATTCAAAT288770.33550060426590117No Hit
CTCCGGAATCGAACCCTTATTCCCCGTTACCCGTTGAAACCATGGTAGGC183580.21328808716672137No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT179270.20828061546126017No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA175130.20347065424070115No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGC167270.19433869887992966No Hit
CTCGTAAGGTGCCGAGTGGGTCATTAAAAAAACACCACCCGATCCCTAGT164250.19082998320696148No Hit
GTCTACAAGAGACCTACCAAGGCCGTCTACAACAAGGCACGCAAGTAGTC148890.1729843299828584No Hit
ATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG147510.1713810095760054No Hit
GTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTT128080.14880672297806774No Hit
GTGCTGGCCTCTAGTTGATAGAATAATGTAGATAAGGGAAGTCGGCAAAA125510.14582082917689945No Hit
GATTAATAGGGACGGTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAA123180.14311377370735778No Hit
CCCTTATTCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCA121330.14096439490106932No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATCAGTACGACCTG118120.1372349322155634No Hit
ATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGCCA117810.13687476603721238No Hit
GTTTATGGTTAAGACTACGACGGTATCTGATCATCTTCGATCCCCTAACT115530.13422580188675956No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT111540.12959011462346717No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105800.12292123119206408No Hit
GTGCCGAGTGGGTCATTAAAAAAACACCACCCGATCCCTAGTCGGCATAG105600.12268886591570857No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTTATTCCCCG103910.12072537933050453No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA100270.11649633130083425No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAG100110.11631043907974986No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC99980.11615940165011877No Hit
GTATTTACATTGTACTCATTCCAATTACAAGACCCGAATGGGCCCTGTAT99180.11522994054469674No Hit
GTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCATCGGCTTGA98680.11464902735380796No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC98290.11419591506491474No Hit
GCACAAGGCCATGCGATTCGAAAAGTTATTATGAATCATCAAAGAGTCCG97420.11318512611276825No Hit
ACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTT97250.11298761562786608No Hit
GTATTTATTAGATAAAAAATCAATGTCTTCGGACTCTTTGATGATTCATA95700.11118678473611089No Hit
GAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCATCGTAATGA93290.10838678315602701No Hit
GAATAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAT93180.10825898225403148No Hit
CCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAA91250.10601665733720082No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACA90400.10502910491268991No Hit
CAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTA90240.10484321269160551No Hit
TGGTAGGCCACTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAAT89700.10421582644544562No Hit
GTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTA86980.1010556586870107No Hit
CTTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGTCTTCGGACTCT86750.10078843861920188No Hit
GTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTAC86120.10005648799868203No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)