FastQCFastQC Report
Tue 3 Aug 2021
H2HY5AFX3_n01_SOR_R_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY5AFX3_n01_SOR_R_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5971120
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATATCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATT174710.29259167459371105No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT126710.21220474550838034No Hit
ATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTT123660.20709682605608326No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT121760.2039148434464556No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT114740.192158255067726No Hit
ATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA105740.17708570586422648No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCT95490.1599197470491298No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC94160.15769235922239042No Hit
CTATAAAGGTAGTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTAC93460.15652004983989604No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA93100.15591714787175603No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC90440.1514623722182773No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAG86870.14548359436755584No Hit
ATTCTACACCCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCA82260.13776309971998554No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACA77960.1305617706560913No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGC77010.1289707793512775No Hit
TCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGA76830.12866932836720749No Hit
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTA76640.12835113010624474No Hit
ATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG75450.12635820415600424No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT75350.12619073138707648No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT74970.1255543348651509No Hit
GACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCA73360.12285802328541379No Hit
AATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATCT72750.12183643939495438No Hit
GTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCA71030.11895590776939668No Hit
GTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGG70220.11759937834108172No Hit
ATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGC69840.1169629818191562No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCTTCGGTAT69660.11666153083508622No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA68800.11522126502230737No Hit
GTATTTACATTGTACTCATTCCAATTACAAGACCCGAATGGGCCCTGTAT68500.11471884671552407No Hit
TGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACC67350.11279290987285467No Hit
CTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCA67340.11277616259596189No Hit
AATAAATACATCTCTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTC67300.11270917348839078No Hit
CAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTA64880.1086563324803387No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTT63900.10701509934484654No Hit
GATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTA63530.10639545009981377No Hit
GATTAATAGGGACGGTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAA62850.10525663527110492No Hit
GTTTATGGTTAAGACTACGACGGTATCTGATCATCTTCGATCCCCTAACT62610.10485470062567825No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGAAGCTCG61790.10348142392047054No Hit
GCACAAGGCCATGCGATTCGAAAAGTTATTATGAATCATCAAAGAGTCCG61550.10307948927504387No Hit
GCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATTCCAGCTC60870.10194067444633502No Hit
GAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTA60810.10184019078497836No Hit
GTATTTATTAGATAAAAAATCAATGTCTTCGGACTCTTTGATGATTCATA60800.10182344350808559No Hit
ATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGCCA60300.10098607966344672No Hit
CTCCGGAATCGAACCCTTATTCCCCGTTACCCGTTGAAACCATGGTAGGC59940.10038317769530673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)