FastQCFastQC Report
Tue 3 Aug 2021
H2HY5AFX3_n01_SC_R_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HY5AFX3_n01_SC_R_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8607138
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTA390400.4535770194459529No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT319750.37149398557336943No Hit
GCTTAATCTTTGAGACAAGCATATGACTACTGGCGATGGTTCATTCAAAT292880.3402757106950069No Hit
GTATAACTGTGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCT194700.22620759653208766No Hit
CTCCGGAATCGAACCCTTATTCCCCGTTACCCGTTGAAACCATGGTAGGC190130.22089804996736428No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGC172070.19991546551246187No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA169220.19660426032439585No Hit
CTCGTAAGGTGCCGAGTGGGTCATTAAAAAAACACCACCCGATCCCTAGT160960.18700757441091337No Hit
GTCTACAAGAGACCTACCAAGGCCGTCTACAACAAGGCACGCAAGTAGTC154100.1790374454319194No Hit
ATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG144610.16801171306885052No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATCAGTACGACCTG133960.15563826210291967No Hit
GTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAAGGGTT131850.1531868084373691No Hit
GTGCTGGCCTCTAGTTGATAGAATAATGTAGATAAGGGAAGTCGGCAAAA131160.15238514823394256No Hit
GATTAATAGGGACGGTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAA127040.1475984235410191No Hit
CCCTTATTCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCA121700.14139427066232702No Hit
ATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGAACCATCGCCA118920.13816439332098543No Hit
GTTTATGGTTAAGACTACGACGGTATCTGATCATCTTCGATCCCCTAACT117890.13696771214775458No Hit
GTGCCGAGTGGGTCATTAAAAAAACACCACCCGATCCCTAGTCGGCATAG114670.13322663119843087No Hit
GAATAATAGAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCAT113560.13193700391465782No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTTATTCCCCG103440.12017932093106908No Hit
CTATTATTCCATGCTAATATATTCGAGCAATACGCCTGCTTTGAACACTC102740.1193660424638248No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAA102540.1191336771874693No Hit
ACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTT102430.11900587628547377No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC102200.11873865621766491No Hit
CAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCCAAG102080.11859923705185162No Hit
GTATTTACATTGTACTCATTCCAATTACAAGACCCGAATGGGCCCTGTAT100260.11648471303701649No Hit
GAATAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAT98940.11495110221307013No Hit
GTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCATCGGCTTGA98600.11455608124326577No Hit
GTATTTATTAGATAAAAAATCAATGTCTTCGGACTCTTTGATGATTCATA96410.11201168146717294No Hit
GCACAAGGCCATGCGATTCGAAAAGTTATTATGAATCATCAAAGAGTCCG95860.1113726769571953No Hit
CCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAA95240.11065234460049321No Hit
CAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTA94680.11000172182669779No Hit
CTTCCAAAGGGTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACA93280.10837516489220922No Hit
TGGTAGGCCACTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAAT92040.10693450017880508No Hit
GTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTA88010.10225233986024158No Hit
CAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGATAACTGATTTA87010.10109051347846404No Hit
GAATAGGACGTTTGGTTCTATTTTGTTGGTTTCTAGGACCATCGTAATGA86880.10093947604883295No Hit
CTTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGTCTTCGGACTCT86760.10080005688301964No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)