FastQCFastQC Report
Thu 16 Sep 2021
H2HW2AFX3_n02_11540.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HW2AFX3_n02_11540.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8044770
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC1969612.4483111387895486No Hit
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGAT894061.1113555763558187No Hit
ACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGG835111.0380781551243852No Hit
ACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT498760.619980434493466No Hit
CTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTT433120.5383870514632488No Hit
CAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCT426570.5302451157708673No Hit
CAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTT416280.5174541969503168No Hit
CGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTCGG392940.4884415589258611No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG377690.4694851437642095No Hit
GCGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTC355300.4416533971760535No Hit
GGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAAT354870.4411188884206758No Hit
GCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATT352310.43793669676075264No Hit
CACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGA349910.4349533920795747No Hit
AGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA328230.40800420645960045No Hit
CCGGAATTGGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGC303160.37684110297746237No Hit
CGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCT302120.3755483376156186No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA300730.3738205069877697No Hit
TACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCG276490.3436891297078723No Hit
CGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAAT255060.31705070499218746No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGT225620.2804555009030712No Hit
CGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCT224720.27933676164762944No Hit
CCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGA220600.27421542194494064No Hit
ATCGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCC203030.25237514559148366No Hit
GCCGAAGTTTCCCTCAGGATAGCAGAAGCTCGTATCAGTTTTATGAGGTA198750.24705491891004963No Hit
AAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAG191050.237483483057937No Hit
GGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTT188400.2341894174724697No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT185930.2311190997380907No Hit
GGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGATAAGGGA184490.22932911692938393No Hit
GGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAAT175650.21834061135371177No Hit
ACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCT175640.2183281809175402No Hit
AACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTC174850.2173461764599858No Hit
CCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGG172450.21436287177880783No Hit
CGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTG171140.2127344846403315No Hit
GTCTGATCAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAA170810.2123242802466696No Hit
CCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGCTGGCTCC168780.2098009017038399No Hit
GCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTTG164890.2049654620330973No Hit
CTTGGATTTATTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTT161350.2005650876283598No Hit
GGCTGGAAGAGGCCAGCACCTTTGCTGGCTCCGGTGCGCTTGTGACGGCC161150.2003164789049283No Hit
TGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAACGAAAGTTAG160670.1997198179686927No Hit
CCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGA157410.1956674957767593No Hit
GGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGC155550.19335543464884639No Hit
CCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATT152690.189800329903776No Hit
CGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGG149920.18635709908424977No Hit
CGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGT148770.18492759892451865No Hit
CGAAGATGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTA148360.1844179510414841No Hit
CGGAGCTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGG142420.17703427195556865No Hit
CTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAG142260.17683538497682347No Hit
CAACTTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGG140560.17472221082765574No Hit
CGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCC139440.17333000197643936No Hit
AACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTC138030.1715773104762473No Hit
CGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCG137760.17124168869961479No Hit
GAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAA137750.1712292582634432No Hit
GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCAC136920.1701975320612025No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTTTGACATTGTGAAGAGACATAG136780.17002350595480045No Hit
CTCGACCCTTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGTCTTC136230.1693398319653638No Hit
AAAAAATCAATGTCTTCGGACTCTTTGATGATTCATAATAACTTTTCGAA133580.1660457663798965No Hit
TGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAA133520.16597118376286704No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGG132500.16470327927336642No Hit
GCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTA131600.1635845400179247No Hit
CAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAAAC130960.1627889921029439No Hit
CGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGTGAA128610.15986783960262382No Hit
CAAGAACGAAAGTTAGGGGATCGAAGATGATCAGATACCGTCGTAGTCTT128460.1596813830600502No Hit
TGATCAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGC127940.1590350003791283No Hit
TGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAA127430.15840104813437797No Hit
CGGGGGCATCAGTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATTGA125370.15584037828303357No Hit
CACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTG124510.15477136077227815No Hit
CACGCTCATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCCGGAC123100.1530186692720861No Hit
CCCGGAATTGGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAG122780.1526208953145957No Hit
CGCTCAAGCGTGTTACCTATACTCTACCGTCAGGGTTGATATGATGCCCT122230.15193722132515908No Hit
TGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCA118690.1475368469204216No Hit
GCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGG117030.14547339451594016No Hit
CGGCGCCAGTGAAATACCACTACCTTTATAGTTTCTTTACTTATTCAATG115970.14415576828175322No Hit
CGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTAAGGTCGG115550.1436336899625471No Hit
GCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGAAGCTCGTATCAGTT115550.1436336899625471No Hit
TGCGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCT115200.143198624696542No Hit
GGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCA115140.14312404207951254No Hit
CTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCT113980.14168211148360985No Hit
CTTCGGGATAAGGATTGGCTCTAAGGGTCGGGTAGTGAGGGCCTTGGTCA113860.14153294624955096No Hit
GCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGA112990.14045149830262393No Hit
CTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGG112650.1400288634727904No Hit
CGAAAGGGAATCCGGTTAAGATTCCGGAACCTGGATATGGATTCTTCACG111200.13822645022791205No Hit
TCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA109280.13583980648296967No Hit
CGTGGGTTAGTCGATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTG106170.13197394083360992No Hit
TGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACT104930.13043256674833462No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGA102570.12749898381184296No Hit
TGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGA101220.12582087492868035No Hit
CGCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGT101090.1256592792584499No Hit
AGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAAC100220.12457783131152289No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100040.12435408346043456No Hit
GGAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTGATCAGGCATT99990.12429193127957666No Hit
TGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCTTTGGAAGAG99940.1242297790987188No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG98110.12195500927932061No Hit
CGACCCTTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGTCTTCGG97820.12159452663034492No Hit
CGGACTCTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGC97540.12124647441754083No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATAC96810.12033905257701587No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG96260.11965537858757926No Hit
GCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGC95870.11917059157688784No Hit
AAAATCTCGACCCTTTGGAAGAGATGTATTTATTAGATAAAAAATCAATG94560.11754220443841155No Hit
CTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATG94400.11734331745966635No Hit
AGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTT92280.11470806499129249No Hit
CGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGCTGGCTCCG92220.11463348237426302No Hit
GGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCG91060.11319155177836036No Hit
CGAGAGGAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTGATCAG89600.11137670809731041No Hit
CTCAGGATAGCAGAAGCTCGTATCAGTTTTATGAGGTAAAGCGAATGATT88700.11025796884186868No Hit
GGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA87870.10922624263962799No Hit
CTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAA87850.10920138176728482No Hit
GGTTTTTGCGGCTGTCTGATCAGGCATTGCCGCGAAGCTACCATCCGCTG87780.10911436871408381No Hit
GTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATG86400.10739896852240648No Hit
CGAACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTAT86140.10707577718194554No Hit
TCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTG85810.10666557278828358No Hit
CAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCCGGACGGTGGCCAT84950.10559655527752813No Hit
CGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTT84850.10547225091581237No Hit
CAGGCGTATTGCTCGAATATATTAGCATGGAATAATAGAATAGGACGTTT83450.1037319898517919No Hit
CTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGGCGTGGAG83410.10368226810710561No Hit
CGAACGTAGAGTTAAGGTGCCGGAATACACGCTCATCAGACACCACAAAA83030.10320991153258577No Hit
GGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTTTG82870.10301102455384056No Hit
CAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAG82730.10283699844743852No Hit
GTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATT82730.10283699844743852No Hit
AACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGT82630.10271269408572278No Hit
GGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAA82260.1022527679473745No Hit
CGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTTGCTCCAC81280.10103458520256017No Hit
GAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGG81190.100922711277016No Hit
GGACCATCGTAATGATTAATAGGGACGGTCGGGGGCATCAGTATTCAATT80690.10030118946843726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)