FastQCFastQC Report
Thu 16 Sep 2021
H2HW2AFX3_n02_11538.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HW2AFX3_n02_11538.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8995822
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGAT821480.9131794737601522No Hit
ACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGG773590.8599436493963531No Hit
ACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT658970.732528945103627No Hit
CTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTT553590.6153856757058999No Hit
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC547330.6084268897272533No Hit
CAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTT503350.5595375275322255No Hit
CACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGA438470.4874151578366046No Hit
CAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCT426110.4736754462238137No Hit
CGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTCGG390840.4344683565326215No Hit
GGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAAT361330.40166423924350664No Hit
GCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATT332610.3697383074053711No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG332320.3694159355309609No Hit
CCGGAATTGGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGC323500.35961138404028004No Hit
GGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGATAAGGGA299230.33263219303361047No Hit
CACAGCCAAGGGAATGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTG283010.31460160060970527No Hit
CGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAAT282780.31434592636448344No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA274540.30518611862262285No Hit
TACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCG270150.30030607542034515No Hit
GCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGGTTCTGACGTGCAAATCG244600.27190400165765843No Hit
GCCGAAGTTTCCCTCAGGATAGCAGAAGCTCGTATCAGTTTTATGAGGTA237090.26355568173758886No Hit
ACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCT228730.25426247873735164No Hit
AACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTC228300.2537844790615021No Hit
ATCGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCC218990.2434352302657834No Hit
AAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAG218340.2427126726162434No Hit
GGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTT217370.24163439427769914No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGT211740.2353759334055298No Hit
CGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCT210550.23405309709329508No Hit
GGCTGGAAGAGGCCAGCACCTTTGCTGGCTCCGGTGCGCTTGTGACGGCC209870.23329719062916096No Hit
CCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGA208210.2314518895549512No Hit
CGAAGATGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTA201660.2241707317018945No Hit
CGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTG198290.220424548195818No Hit
CCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATT195150.21693403893496338No Hit
GCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTTG185820.2065625575961819No Hit
GTCTGATCAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAA175450.19503498401813643No Hit
CCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGCTGGCTCC172640.19191131171781745No Hit
GGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGC163230.1814509002067849No Hit
TGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACT162790.18096178425940398No Hit
CTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAG160860.17881634385384682No Hit
CCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGG160350.17824941400574623No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT158950.17669313599135245No Hit
GCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTA155900.17330267317428025No Hit
CGAAAGGGAATCCGGTTAAGATTCCGGAACCTGGATATGGATTCTTCACG154150.171357325656288No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150090.166844119414546No Hit
CGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCC149880.1666106777123869No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG149560.1662549570233826No Hit
CGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCG147740.1642317956046707No Hit
GCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGAAGCTCGTATCAGTT147440.1638983074587292No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG146120.16243095961658646No Hit
AACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGT144020.16009654259499576No Hit
CAACTTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGG143630.15966300800527178No Hit
CCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGA141840.157673195401154No Hit
CGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGG141640.15745086997052632No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGA140040.15567226652550484No Hit
CGATCGTCGAATTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGT139610.15519426684965532No Hit
AGCGAAACCACAGCCAAGGGAATGGGCTTGGCAGAATCAGCGGGGAAAGA139580.15516091803506116No Hit
CACGCTCATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCCGGAC139260.15480519734605686No Hit
CCCGGAATTGGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAG138810.15430496512714456No Hit
TGCTTTTTGATTCTTCGATGTCGGCTCTTCCTATCATACCGAAGCAGAAT136590.15183715284717728No Hit
CGTGGGTTAGTCGATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTG136410.15163705995961235No Hit
CTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGG135880.151047897568449No Hit
CGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGT133900.14884687580523492No Hit
CGCTCAAGCGTGTTACCTATACTCTACCGTCAGGGTTGATATGATGCCCC133800.1487357130899211No Hit
CGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGA131180.1458232499486984No Hit
CGGGGGCATCAGTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATTGA131000.1456231570611335No Hit
CAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAAAC130950.14556757570347656No Hit
TGATCAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGC129970.1444781810934009No Hit
AACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTC127400.14162129930983516No Hit
GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCAC127030.14120999726317396No Hit
GCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGG124950.13889781278464602No Hit
CCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAG124710.1386310222678928No Hit
CTTGGATTTATTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTT124300.13817525513510606No Hit
CTCGACCCTTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGTCTTC123170.13691911645205965No Hit
CTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCT122440.1361076286302686No Hit
CTCCGTTTCAAAGGCCTGATTTTATGCAGGCCACCATCGAAAGGGAATCC121940.13555181505369937No Hit
CGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGTGAA121900.13550734996757383No Hit
TGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAACGAAAGTTAG121050.13456246688740617No Hit
CGAACGTAGAGTTAAGGTGCCGGAATACACGCTCATCAGACACCACAAAA117270.13036051624854295No Hit
CAAGAACGAAAGTTAGGGGATCGAAGATGATCAGATACCGTCGTAGTCTT116650.12967130741359711No Hit
GGAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTGATCAGGCATT116270.12924888909540452No Hit
GGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCA115340.12821507584298578No Hit
CTTCGGGATAAGGATTGGCTCTAAGGGTCGGGTAGTGAGGGCCTTGGTCA114930.12775930871019903No Hit
GCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGA114780.12759256463722826No Hit
GGATTCTTCACGGTAACGTAACTGAATGTGGAGACGTCGGCGCGAGCCCT114300.1270589836037218No Hit
CGCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGT113440.12610298425202277No Hit
TGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAA112490.12504693845654127No Hit
CGGAGCTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGG110050.12233456820288352No Hit
AGTTTTCATGCCAGGTCGTACTGATAACCGCAGCAGGTCTCCAAGGTGAA109720.12196773124234783No Hit
TCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTG109040.12121182477821371No Hit
GCGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTC108560.12067824374470727No Hit
CTCTGGTGGAGGCTCGTAGCGGTTCTGACGTGCAAATCGATCGTCGAATT108080.12014466271120083No Hit
GGACCATCGTAATGATTAATAGGGACGGTCGGGGGCATCAGTATTCAATT106330.11819931519320857No Hit
CTCAGGATAGCAGAAGCTCGTATCAGTTTTATGAGGTAAAGCGAATGATT105650.11744340872907445No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTTTGACATTGTGAAGAGACATAG104060.11567592155558436No Hit
CGAACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTAT102790.11426415507109855No Hit
GCCAAGGGAATGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGTTGA102250.11366387640840382No Hit
CAGATGTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAA102160.11356382996462135No Hit
GCCCCGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTA102010.11339708589165058No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGG101770.11313029537489737No Hit
AGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAAC101680.11303024893111491No Hit
GGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTTTG101520.11285238858661276No Hit
CGAAACCACAGCCAAGGGAATGGGCTTGGCAGAATCAGCGGGGAAAGAAG101340.11265229569904785No Hit
TGATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATA101210.11250778416913985No Hit
CGAGAGGAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTGATCAG101190.11248555162607708No Hit
GCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGC101090.11237438891076323No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATAC101020.11229657501004356No Hit
CGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCT100570.11179634279113125No Hit
CTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATG100000.11116271531384236No Hit
TCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA99560.11067359936646146No Hit
CGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCT99350.11044015766430237No Hit
TGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCTTTGGAAGAG97820.1087393681200006No Hit
CGGCTCTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTC97670.10857262404702983No Hit
GGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAA97400.10827248471568245No Hit
AAAAAATCAATGTCTTCGGACTCTTTGATGATTCATAATAACTTTTCGAA97390.10826136844415107No Hit
GTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATT97230.10808350809964892No Hit
CAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAG94280.10480420799789057No Hit
CCGCAATAGTAATTGAACTTAGTACGAGAGGAACAGTTCATTCGGATAAT94250.10477085918329643No Hit
GCCTCTAGTTGATAGAATAATGTAGATAAGGGAAGTCGGCAAAATAGATC93830.10430397577897829No Hit
CGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGCTGGCTCCG93790.10425951069285276No Hit
CAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCCGGACGGTGGCCAT93740.10420392933519582No Hit
AGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA93120.10351472050025001No Hit
AGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAA92850.10321458116890263No Hit
GTCGGCGCGAGCCCTGGGAGGAGTTATCTTTTCTTCTTAACAGCTTATCA92650.10299225573827495No Hit
CGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTT92390.10270323267845896No Hit
CTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAA92380.10269211640692756No Hit
AGTTGATAGAATAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAAC92250.10254760487701958No Hit
TGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGAT92010.10228081436026634No Hit
ACCGAACGTAGAGTTAAGGTGCCGGAATACACGCTCATCAGACACCACAA91250.10143597772388116No Hit
CGTGCAAATCGATCGTCGAATTTGGGTATAGGGGCGAAAGACTAATCGAA90850.10099132686262578No Hit
CGCTCATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCCGGACGG90680.10080235024659225No Hit
CACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGT90390.10047997837218212No Hit
CGAGCCCTGGGAGGAGTTATCTTTTCTTCTTAACAGCTTATCACCCCGGA90280.1003576993853369No Hit
GGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA90150.10021318785542889No Hit
CTCTAGTTGATAGAATAATGTAGATAAGGGAAGTCGGCAAAATAGATCCG90030.10007979259705228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)