FastQCFastQC Report
Thu 16 Sep 2021
H2HW2AFX3_n02_11537.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HW2AFX3_n02_11537.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10814961
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGG895570.8280843546268913No Hit
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC822690.7606962244246651No Hit
GGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAAT761010.7036641186223418No Hit
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGAT709700.6562205818402859No Hit
ACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT612940.5667519281854091No Hit
CTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTT574610.5313102839668122No Hit
GCGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTC361560.3343146591097277No Hit
CAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTT345870.3198069784995064No Hit
CACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGA333650.30850781616318357No Hit
GCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATT305760.28271946611735355No Hit
CAACTTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGG300370.2777356293749002No Hit
ATCGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCC291010.2690809518406955No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG287140.26550257555251466No Hit
TACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCG281170.2599824446893521No Hit
GGCTGGAAGAGGCCAGCACCTTTGCTGGCTCCGGTGCGCTTGTGACGGCC279070.25804069011437025No Hit
GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCAC273570.25295514241798933No Hit
AACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTC273230.2526407631058494No Hit
GCCAGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTC266030.24598331884876884No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA257600.2381885611977704No Hit
CGAAGATGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTA231350.21391662901049757No Hit
ACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCT230430.2130659555776484No Hit
CAAGAACGAAAGTTAGGGGATCGAAGATGATCAGATACCGTCGTAGTCTT228190.21099475069766782No Hit
GCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGA223610.20675987643413601No Hit
GCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTA219600.2030520498409564No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAA214150.19801273439636075No Hit
CGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCT211850.19588605081423777No Hit
CCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGA209510.19372238143068662No Hit
GGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGC204700.18927483880894255No Hit
GGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGATAAGGGA204130.18874779113859033No Hit
GCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCTT203920.18855361568109213No Hit
CGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTCGG203020.1877214351489571No Hit
GGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCA202480.18722212682967604No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGT201750.18654713595361092No Hit
GATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACC201380.18620501729039984No Hit
AAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAG196850.18201637527865336No Hit
GGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCC195100.18039824646616848No Hit
CGCTCAAGCGTGTTACCTATACTCTACCGTCAGGGTTGATATGATGCCCT189730.17543290262442926No Hit
CCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGA189250.17498907300729055No Hit
AGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGT188830.17460072209229419No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG188300.1741106602233702No Hit
CGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCT187690.17354662675158977No Hit
AAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCAC185630.17164185797803616No Hit
CGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGTGAA182140.16841484680342353No Hit
GTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTG180670.16705561860093623No Hit
CACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGT180050.16648233867879875No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG177410.1640412757845359No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGG174870.16169267739384358No Hit
CGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGG174620.16146151613491716No Hit
TGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAACGAAAGTTAG173130.16008379503171577No Hit
TTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAA172700.15968619766636236No Hit
GTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAA172630.15962147251386297No Hit
CGAACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTAT171120.15822525850994745No Hit
CCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGCTGGCTCC170800.15792937209852168No Hit
AACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTC170010.15719890252031424No Hit
GGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTA168850.1561263142788957No Hit
AAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACCTTTATAGTTTCTTT168310.15562700595961465No Hit
GAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATG165210.15276060634892719No Hit
GCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTA163790.15144761039822519No Hit
CAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGG163230.15092980917823004No Hit
CTTGGATTTATTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTT162710.1504489937596631No Hit
CAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCT162360.15012536799716616No Hit
CTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGG161880.14968153838002743No Hit
GACGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATCAGA159620.14759184059933272No Hit
AACGAAAGTTAGGGGATCGAAGATGATCAGATACCGTCGTAGTCTTAACC159480.14746239029433392No Hit
GGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTT157660.14577953632934967No Hit
GCGAATGATTAGAGGTTCCGGGGTCGAAATGACCTTGACCTATTCTCAAA157250.14540043186471038No Hit
AAAAAATCAATGTCTTCGGACTCTTTGATGATTCATAATAACTTTTCGAA156040.14428161137150655No Hit
AGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAAC155130.14344018438901446No Hit
CGAAAGGGAATCCGGTTAAGATTCCGGAACCTGGATATGGATTCTTCACG154950.14327374828258743No Hit
GCCGAAGTTTCCCTCAGGATAGCAGAAGCTCGTATCAGTTTTATGAGGTA154820.1431535444279457No Hit
GTCGGCGCGAGCCCTGGGAGGAGTTATCTTTTCTTCTTAACAGCTTATCA154550.14290389026830516No Hit
GCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGGTTCTGACGTGCAAATCG154080.14246930710152353No Hit
GAGGCCAGCACCTTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAA152190.14072172798403987No Hit
CGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGT151710.1402778983669012No Hit
GCTCAAGCGTGTTACCTATACTCTACCGTCAGGGTTGATATGATGCCCTG151270.1398710545511907No Hit
CCGGAATTGGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGC150890.13951968943762258No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAAC149160.13792005352585182No Hit
GTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATT149000.13777211032013892No Hit
AGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAA146530.13548823708194602No Hit
GTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATG145690.13471153525195328No Hit
AGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA144690.13378689021624765No Hit
CTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATG144250.1333800464005372No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTTTGACATTGTGAAGAGACATAG143810.1329732025848267No Hit
CGGAGCTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGG142950.13217800785411984No Hit
GCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGTGAACGTAGGT142060.13135507377234185No Hit
CCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGG141500.1308372725523467No Hit
CGTGGGTTAGTCGATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTG139670.12914517213700538No Hit
GTTCCACGTCAACAGCAGTTGGACGTGGGTTAGTCGATCCTAAGAGATGG139480.12896948958022134No Hit
CGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTAAGGTCGG136830.1265191802356014No Hit
CTTCGGGATAAGGATTGGCTCTAAGGGTCGGGTAGTGAGGGCCTTGGTCA136520.12623254027453265No Hit
GCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTC136090.12583494290917924No Hit
CGCAGATGTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAAC136070.1258164500084651No Hit
TGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCA135760.12552981004739638No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA135730.1255020706963252No Hit
CGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTT135540.12532638813954114No Hit
GGACCATCGTAATGATTAATAGGGACGGTCGGGGGCATCAGTATTCAATT134060.12395791348669681No Hit
GGCCAGCACCTTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATC132730.12272813558920831No Hit
GTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAAA132250.12228430597206962No Hit
CGGGGAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA131670.12174801185136036No Hit
CGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCT131160.12127644288315048No Hit
GGTTGATATGATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAA131100.12122096418100814No Hit
GTTCTGGGCCGCACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTT130630.1207863810142265No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG130230.12041652299994424No Hit
CTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAG126800.11724499052747393No Hit
GGACGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATCAG126560.11702307571890458No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGA124480.11509981404463686No Hit
GTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACC124430.11505358179285158No Hit
GCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGG123040.11376832519322075No Hit
TGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATC121990.11279744790572985No Hit
GGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTG121750.1125755330971605No Hit
AGACGTTCTGGGCCGCACGCGCGCTACACTGACGGAGCCAGCGAGTCTAA121040.1119190351218095No Hit
GAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCTATACTCTAC120620.11153068420681313No Hit
GGATTCTTCACGGTAACGTAACTGAATGTGGAGACGTCGGCGCGAGCCCT120100.1110498687882462No Hit
GCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTTG120070.11102212943717504No Hit
TGATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGAC119940.11090192558253331No Hit
AACGAACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTT119300.11031015275968171No Hit
AACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGT119260.11027316695825348No Hit
GGGAAACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTC118290.10937626127361902No Hit
GACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTTTGTGGGTG118020.10912660711397851No Hit
CTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAG117140.10831291948255753No Hit
CGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTG116920.1081094975747023No Hit
TGCGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCT116800.10799854017041763No Hit
GCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTC116690.10789682921649002No Hit
GGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA116460.10768416085827771No Hit
CGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGT116070.10732354929435253No Hit
CCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATT115950.10721259189006783No Hit
GGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAAT115780.10705540223399788No Hit
TGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAG115030.10636191845721865No Hit
CTTTTCTTCTTAACAGCTTATCACCCCGGAATTGGTTTATCCGGAGATGG114780.10613075719829225No Hit
GAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAG114700.10605678559543581No Hit
GGTCAGTGACGAAGCCTAGACCGTAAGGTCGGGTCGAACGGCCTCTAGTG114400.10577939208472412No Hit
GTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAA113650.10508590830794488No Hit
AGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGA112780.10428146712688098No Hit
CAGATGTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAA112060.10361572270117293No Hit
CACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTG110410.10209005839225864No Hit
AGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAA108880.10067535148762903No Hit
CGTAACTGAATGTGGAGACGTCGGCGCGAGCCCTGGGAGGAGTTATCTTT108190.10003734641299215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)