FastQCFastQC Report
Thu 16 Sep 2021
H2HW2AFX3_n02_11534.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HW2AFX3_n02_11534.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9373564
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGG777220.829161672123858No Hit
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGAT633520.6758581901185078No Hit
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC582730.6216738905287252No Hit
ACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT546590.5831186515609218No Hit
CTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTT411830.4393526304402466No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG398370.4249930976094045No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG396250.42273141784704305No Hit
CACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGA369150.3938203227715733No Hit
CAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTT365680.39011842240582134No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA362050.3862458292278156No Hit
GGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAAT344830.36787501530901157No Hit
CAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCT304770.32513780244099255No Hit
GCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATT291670.31116232843772124No Hit
TACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCG272020.29019911743281424No Hit
CCGGAATTGGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGC263080.280661656548139No Hit
GGCTGGAAGAGGCCAGCACCTTTGCTGGCTCCGGTGCGCTTGTGACGGCC220800.23555608091009994No Hit
CGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCT217610.2321528929657919No Hit
CACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTG214430.22876037332224966No Hit
ATCGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCC212860.2270854501020103No Hit
ACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCT208140.22205001214052628No Hit
AACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTC205120.21882818530923778No Hit
GCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGGTTCTGACGTGCAAATCG204410.218070735954862No Hit
CGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTCGG203530.21713192548746665No Hit
GGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGATAAGGGA202740.21628912972696404No Hit
CGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAAT197340.2105282473134018No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT188410.20100145472949243No Hit
CCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGCTGGCTCC188170.20074541551111189No Hit
CCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGA185320.19770494979284292No Hit
GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCAC182160.19433376675083244No Hit
GCCGAAGTTTCCCTCAGGATAGCAGAAGCTCGTATCAGTTTTATGAGGTA181850.1940030494270909No Hit
GCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTTG181730.19387502981790064No Hit
AAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAG180840.19292555104973946No Hit
GCGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTC179680.19168802816090016No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGT177290.1891383042778606No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG176840.18865823074339708No Hit
GTCTGATCAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAA172670.18420954932403513No Hit
GGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTT172100.18360145618038132No Hit
GCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTA171760.18323873395434223No Hit
CGAAGATGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTA166450.1775738662476727No Hit
AACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGT165250.17629367015577No Hit
GGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGC158640.1692419233495392No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG158240.16881519131890496No Hit
TCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA153410.1636624020489965No Hit
CCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATT152770.16297963079998173No Hit
CCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGA146680.15648263563357545No Hit
AACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTC146180.15594922059528263No Hit
CGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTG144490.154146277765853No Hit
CCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGG142960.15251402774867703No Hit
GCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGA140210.14958024503806663No Hit
TGCTTTTTGATTCTTCGATGTCGGCTCTTCCTATCATACCGAAGCAGAAT139150.1484494051568859No Hit
CAACTTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGG137430.1466144574251587No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATAC136440.14555829564933892No Hit
TGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAACGAAAGTTAG136260.14536626623555352No Hit
CGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGG136150.1452489149271291No Hit
CAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAAAC132260.14109894592921113No Hit
CACGCTCATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCCGGAC131210.13997877434879627No Hit
CGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTAAGGTCGG129780.13845320733927885No Hit
CGGAGCTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGG128110.13667160111138088No Hit
CGAAAGGGAATCCGGTTAAGATTCCGGAACCTGGATATGGATTCTTCACG125380.1337591550023022No Hit
GCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGC123800.13207356348129698No Hit
CGCTCAAGCGTGTTACCTATACTCTACCGTCAGGGTTGATATGATGCCCT120700.12876639024388162No Hit
GCGAATGATTAGAGGTTCCGGGGTCGAAATGACCTTGACCTATTCTCAAA120390.12843567292014008No Hit
CCGCAATAGTAATTGAACTTAGTACGAGAGGAACAGTTCATTCGGATAAT119890.12790225788184728No Hit
CGTGGGTTAGTCGATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTG118740.12667540329377386No Hit
CGAACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTAT118690.1266220617899446No Hit
AGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAA117790.12566191472101754No Hit
CTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGG117510.12536320229957357No Hit
GCCAGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTC116750.12455241144136851No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGA114750.12241875128819733No Hit
GCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGG114630.12229073167900703No Hit
CGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGT114540.12219471697211436No Hit
CCCGGAATTGGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAG114420.12206669736292407No Hit
GGATTCTTCACGGTAACGTAACTGAATGTGGAGACGTCGGCGCGAGCCCT112600.12012506662353829No Hit
AGTTTTCATGCCAGGTCGTACTGATAACCGCAGCAGGTCTCCAAGGTGAA112440.11995437381128457No Hit
GCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGAAGCTCGTATCAGTT111730.1191969244569088No Hit
CGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGTGAA111560.11901556334388926No Hit
GGAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTGATCAGGCATT111460.1189088803362307No Hit
CTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAG111100.11852482150865988No Hit
CGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCG110170.1175326695374353No Hit
CGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCT109160.11645517116008383No Hit
CGGCTCTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTC108380.11562304370034705No Hit
AGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGA107890.11510029696282012No Hit
CGAGAGGAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTGATCAG107210.1143748525107419No Hit
GCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTA107180.11434284760844435No Hit
GGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCA107070.11422549630001992No Hit
CAAGAACGAAAGTTAGGGGATCGAAGATGATCAGATACCGTCGTAGTCTT106030.11311599302037091No Hit
CGATCGTCGAATTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGT105590.11264658778667325No Hit
CGCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGT105320.11235854366599514No Hit
GCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGTTGA105240.11227319725986828No Hit
TGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACT105150.11217718255297558No Hit
AGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAAC105140.11216651425220972No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGG104800.11180379202617062No Hit
CTTGGATTTATTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTT102940.10981948808372141No Hit
GTCGGCGCGAGCCCTGGGAGGAGTTATCTTTTCTTCTTAACAGCTTATCA102860.10973414167759457No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA102630.10948877075997987No Hit
ACCGAACGTAGAGTTAAGGTGCCGGAATACACGCTCATCAGACACCACAA102530.1093820877523213No Hit
CGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCC101540.10832592597650158No Hit
CGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCT101540.10832592597650158No Hit
CGAACGTAGAGTTAAGGTGCCGGAATACACGCTCATCAGACACCACAAAA101130.10788852564510149No Hit
TGATCAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGC100880.10762181812595509No Hit
AGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA100640.10736577890757452No Hit
CTCTGGTGGAGGCTCGTAGCGGTTCTGACGTGCAAATCGATCGTCGAATT100390.10709907138842813No Hit
CAGATGTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAA100390.10709907138842813No Hit
GCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTC99220.10585088019882298No Hit
CTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATG98760.1053601383635936No Hit
CGTGGAGGTCAGTGACGAAGCCTAGACCGTAAGGTCGGGTCGAACGGCCT98690.10528546025823263No Hit
CGTAACTGAATGTGGAGACGTCGGCGCGAGCCCTGGGAGGAGTTATCTTT98400.10497607953602281No Hit
GCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGG96950.10342917592497368No Hit
GGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCC96510.10295977069127603No Hit
GGTTTTTGCGGCTGTCTGATCAGGCATTGCCGCGAAGCTACCATCCGCTG96300.10273573637519305No Hit
CACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGT96200.10262905336753449No Hit
TGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCTTTGGAAGAG96020.10243702395374907No Hit
CCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAG95860.10226633114149537No Hit
AGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTT95230.10159422819324646No Hit
CTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAA94660.10098613504959267No Hit
TTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAA94400.10070875922968041No Hit
GCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTC94090.10037804190593887No Hit
GGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA93900.10017534419138761No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)