FastQCFastQC Report
Thu 16 Sep 2021
H2HW2AFX3_n02_11241.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HW2AFX3_n02_11241.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9141548
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC1256471.3744608681155532No Hit
ACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGG970901.0620739507138177No Hit
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGAT743560.8133852165957013No Hit
GGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAAT618010.6760452387276203No Hit
ACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT525480.5748260579061665No Hit
GCGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTC434120.4748867478462072No Hit
CTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTT388050.42449046922906275No Hit
CACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGA380110.41580485055703914No Hit
CAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTT373810.40891323876437563No Hit
TACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCG323640.354031942948831No Hit
GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCAC295160.32287748201945665No Hit
GCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATT283160.30975060241438324No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG271180.2966456009419849No Hit
ATCGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCC251680.27531442158374053No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG248290.2716060780953073No Hit
ACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCT236430.2586323454189597No Hit
CAAGAACGAAAGTTAGGGGATCGAAGATGATCAGATACCGTCGTAGTCTT235740.25787754984166794No Hit
CGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGTGAA232620.25446456114434884No Hit
CAACTTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGG231610.2533597154442552No Hit
CAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCT220540.2412501690085749No Hit
AGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA218310.23881075721529876No Hit
CGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCT216910.23727928792804018No Hit
CGAAGATGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTA214160.23427104468521087No Hit
AACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTC212910.23290366139301572No Hit
TGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAACGAAAGTTAG206840.22626364812611605No Hit
CCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGA206740.22615425746274043No Hit
CTTGGATTTATTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTT203720.22285065942879695No Hit
CGCTCAAGCGTGTTACCTATACTCTACCGTCAGGGTTGATATGATGCCCT203340.22243497490796962No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA200190.21898916901163784No Hit
CGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTCGG199560.21830000783237152No Hit
CGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCT193900.21210849628531184No Hit
TGCGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCT187400.2049981031658971No Hit
TGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCA186110.20358696360835166No Hit
AAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAG184230.20153041913689015No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGT180050.1969578894077896No Hit
CCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGA177940.19464974641056418No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGG176580.19316203338865584No Hit
GGCTGGAAGAGGCCAGCACCTTTGCTGGCTCCGGTGCGCTTGTGACGGCC176330.19288855673021682No Hit
CGAACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTAT174520.19090858572311822No Hit
GCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTA172540.1887426505882811No Hit
GCCAGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTC167270.18297776262838636No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAA166200.1818072825302673No Hit
CGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGG165570.18111812135100094No Hit
CACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGT164670.18013360538062045No Hit
GGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGATAAGGGA162970.178273964103235No Hit
GGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGC161650.17683000734667695No Hit
GCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGA158450.17332950611865738No Hit
AACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTC156700.17141516950958416No Hit
AACGAAAGTTAGGGGATCGAAGATGATCAGATACCGTCGTAGTCTTAACC156450.1711416928511451No Hit
GCCGAAGTTTCCCTCAGGATAGCAGAAGCTCGTATCAGTTTTATGAGGTA152130.16641601619331867No Hit
CCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGG151310.16551901275363864No Hit
GCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGTGAACGTAGGT149750.1638125184049791No Hit
GACGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATCAGA147730.16160282700479175No Hit
GCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTA147240.16106681275425125No Hit
GGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAAT146690.16046516410568537No Hit
CGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGT145210.15884618228772632No Hit
CTTCGGGATAAGGATTGGCTCTAAGGGTCGGGTAGTGAGGGCCTTGGTCA142680.15607859850432335No Hit
CGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTAAGGTCGG142440.15581606091222186No Hit
CGAAAGGGAATCCGGTTAAGATTCCGGAACCTGGATATGGATTCTTCACG140600.1538032727061106No Hit
CCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGCTGGCTCC140480.15367200391005986No Hit
CGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTT140170.15333289285359547No Hit
CAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGG139980.15312505059318182No Hit
GAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATG139580.15268748793967934No Hit
CGGGGGCATCAGTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATTGA139290.15237025501589008No Hit
GGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCC139290.15237025501589008No Hit
GCTCAAGCGTGTTACCTATACTCTACCGTCAGGGTTGATATGATGCCCTG139100.15216241275547643No Hit
GCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCTT138750.1517795454336618No Hit
CTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGG137660.1505871872028676No Hit
AAAAAATCAATGTCTTCGGACTCTTTGATGATTCATAATAACTTTTCGAA136230.14902290071659635No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA135710.1484540692670432No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTTTGACATTGTGAAGAGACATAG135620.14835561767000513No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG134910.14757894396003826No Hit
CGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTG133320.14583963241236605No Hit
AGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAAC132830.14530361816182555No Hit
AGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAA132410.14484417737564798No Hit
CGGAGCTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGG131970.14436285845679528No Hit
CGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCT130440.14268918130714842No Hit
CGCAGATGTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAAC129790.14197814199520695No Hit
CGTGGGTTAGTCGATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTG129470.14162809187240497No Hit
CGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCT127770.13976845059501958No Hit
GATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACC127250.13919961914546639No Hit
AGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTT126520.13840106730282442No Hit
CCGGAATTGGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGC123800.13542564125900777No Hit
AGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAA123000.13455051595200287No Hit
AGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGT121900.13334721865487115No Hit
GTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATT121790.13322688892515797No Hit
GCGAATGATTAGAGGTTCCGGGGTCGAAATGACCTTGACCTATTCTCAAA121680.13310655919544478No Hit
CGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAAT120500.13181574936761256No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT119220.13041554887640475No Hit
GGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCA119080.13026240194767888No Hit
CTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAG118310.12942009383968667No Hit
TGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATC118240.12934352037532376No Hit
GGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTA118190.12928882504363595No Hit
CAGATGTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAA117760.12881844519112082No Hit
TGATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGAC117520.12855590759901933No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAAC116870.12784486828707786No Hit
CCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATT116460.12739636656723785No Hit
AGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGA115490.12633527713249443No Hit
GCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGGTTCTGACGTGCAAATCG115470.12631339899981928No Hit
TTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAA115320.12614931300475588No Hit
GTTCTGGGCCGCACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTT115210.1260289832750427No Hit
GAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAA115180.12599616607603No Hit
CTTTTCTTCTTAACAGCTTATCACCCCGGAATTGGTTTATCCGGAGATGG114140.12485850317692365No Hit
CTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATG113940.12463972185017243No Hit
TGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGA113940.12463972185017243No Hit
CGGGGAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA113700.12437718425807096No Hit
GAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCTATACTCTAC112930.12353487615007874No Hit
GGTTGATATGATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAA112890.1234911198847285No Hit
GGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTT111620.12210185845985821No Hit
TGTGAAGAGACATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTG111610.12209091939352065No Hit
GCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTC110730.12112828155581526No Hit
CGCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGT108360.11853572283381326No Hit
AGACGTTCTGGGCCGCACGCGCGCTACACTGACGGAGCCAGCGAGTCTAA107620.11772623192483372No Hit
GTTCCACGTCAACAGCAGTTGGACGTGGGTTAGTCGATCCTAAGAGATGG106610.11662138622474005No Hit
CACGCTCATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCCGGAC106370.11635884863263858No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGA106040.11599785944349907No Hit
GCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTTG105330.11522118573353222No Hit
CGTAACTGAATGTGGAGACGTCGGCGCGAGCCCTGGGAGGAGTTATCTTT105100.11496958720776831No Hit
CGTCGGCGCGAGCCCTGGGAGGAGTTATCTTTTCTTCTTAACAGCTTATC105080.11494770907509318No Hit
GGACGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATCAG104370.11417103536512635No Hit
CGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATCAGATA104340.11413821816611366No Hit
CCGAAAGATGGTGAACTATGCCTGAATAGGGTGAAGCCAGAGGAAACTCT104250.1140397665690756No Hit
TGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCGGCC103520.11324121472643363No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGA101220.11072522946879457No Hit
CGGACTCTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGC99320.10864680686465793No Hit
CGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGT98900.10818736607848035No Hit
GGATTCTTCACGGTAACGTAACTGAATGTGGAGACGTCGGCGCGAGCCCT98690.10795764568539158No Hit
CAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCACGCGCGCTACACTGA98130.10734505797048814No Hit
CTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTG97600.10676528745459742No Hit
AGATGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGG97180.10630584666841984No Hit
GCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGC97140.10626209040306958No Hit
CTCGACCCTTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGTCTTC96590.10566044175450372No Hit
AAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCAC96510.10557292922380324No Hit
CGAGCCCTGGGAGGAGTTATCTTTTCTTCTTAACAGCTTATCACCCCGGA96320.10536508696338956No Hit
AACGAACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTT95900.10490564617721199No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG94930.10384455674246856No Hit
GGACCATCGTAATGATTAATAGGGACGGTCGGGGGCATCAGTATTCAATT94120.10295849236912612No Hit
CACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTG94090.10292567517011342No Hit
GCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTC92140.10079255723428898No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)