FastQCFastQC Report
Thu 16 Sep 2021
H2HW2AFX3_n02_11222.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HW2AFX3_n02_11222.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9409635
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC891490.9474225089496033No Hit
ACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGG817640.8689391246312955No Hit
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGAT701070.7450554670824107No Hit
ACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT518230.5507439980403065No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG416640.4427801928555146No Hit
CTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTT383840.40792230516911654No Hit
CACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGA376060.39965418424837945No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG375810.39938849912881846No Hit
CAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTT373510.39694419602885767No Hit
CGAAGATGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTA348500.37036505666797914No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA326780.3472823334805229No Hit
GCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATT305460.32462470648436414No Hit
CGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTCGG290920.3091724599306987No Hit
TGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAACGAAAGTTAG283630.30142508184430106No Hit
GGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAAT265320.2819663036876563No Hit
TACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCG264510.28110548390027884No Hit
CCGGAATTGGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGC261600.2780129091085892No Hit
GGCTGGAAGAGGCCAGCACCTTTGCTGGCTCCGGTGCGCTTGTGACGGCC252040.2678531101365781No Hit
CAAGAACGAAAGTTAGGGGATCGAAGATGATCAGATACCGTCGTAGTCTT240720.2558228879228578No Hit
CAACTTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGG234700.24942519024382984No Hit
GCGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTC232660.2472571996682124No Hit
AACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTC228270.24259176896872198No Hit
GCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGA225170.23929727348616603No Hit
GCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTTG223870.23791571086444904No Hit
CAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCT212430.2257579597933395No Hit
CGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCT209770.22293107012121088No Hit
GGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTT207250.2202529641160364No Hit
ACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCT202450.21515180982046594No Hit
GCCAGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTC201950.21462043958134402No Hit
GCCGAAGTTTCCCTCAGGATAGCAGAAGCTCGTATCAGTTTTATGAGGTA200330.212898800006589No Hit
CCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGA192100.20415244587064216No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGG191620.2036423304410851No Hit
ATCGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCC189450.20133618360329597No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGT187030.19876435164594589No Hit
CCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGCTGGCTCC184070.1956186398303441No Hit
CGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAAT183470.19498099554339782No Hit
AACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTC181950.19336563001646717No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT178080.18925282436566349No Hit
CCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGA177210.18832824014959135No Hit
GGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGATAAGGGA172110.18290826371054775No Hit
CGGAGCTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGG166560.1770100540562944No Hit
CGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGG165260.17562849143457743No Hit
AGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA159940.1699747120903202No Hit
GCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAACGAAAGTTAGG158440.16838060137295444No Hit
CTTGGATTTATTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTT158320.16825307251556518No Hit
CTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATG154650.16435281496041027No Hit
GCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGG151740.16126024016872068No Hit
CAGATGTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAA150190.1596129924274427No Hit
CCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATT150090.15950671837961833No Hit
GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCAC149490.15886907409267204No Hit
CGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGT149410.15878405485441255No Hit
CCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGG146890.15610594884923806No Hit
AGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGT143780.15280082596189967No Hit
CGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCT140450.14926190016934768No Hit
TGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCA140020.14880492176370283No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG138660.14735959471329121No Hit
GGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGC136710.14528725078071572No Hit
GCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGGTTCTGACGTGCAAATCG136190.14473462573202892No Hit
CGCTCAAGCGTGTTACCTATACTCTACCGTCAGGGTTGATATGATGCCCT135040.14351247418204852No Hit
GGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCC134910.14337431791987681No Hit
GACGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATCAGA134860.14332118089596463No Hit
TTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAA134360.1427898106568427No Hit
CGCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGT133810.14220530339380857No Hit
CGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCT133000.14134448360643107No Hit
CAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGG132240.14053680084296574No Hit
AAAAAATCAATGTCTTCGGACTCTTTGATGATTCATAATAACTTTTCGAA131790.140058567627756No Hit
TCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA129370.13748673567040592No Hit
CGGGGGCATCAGTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATTGA129280.13739108902736397No Hit
CGTGGGTTAGTCGATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTG128100.13613705526303624No Hit
CACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTG128000.13603078121521187No Hit
CTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGG127560.13556317540478455No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG127320.13530811769000606No Hit
TGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAA126320.1342453772117622No Hit
GCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTA126320.1342453772117622No Hit
GCGAATGATTAGAGGTTCCGGGGTCGAAATGACCTTGACCTATTCTCAAA125840.13373526178220516No Hit
GCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGG124180.13197111258832037No Hit
CTTCGGGATAAGGATTGGCTCTAAGGGTCGGGTAGTGAGGGCCTTGGTCA123920.131694800063977No Hit
CGTGGAGGTCAGTGACGAAGCCTAGACCGTAAGGTCGGGTCGAACGGCCT122400.13007943453704635No Hit
CGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTG121530.12915485032097418No Hit
CGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTAAGGTCGG120670.12824089350968448No Hit
AACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGT120050.1275819944131733No Hit
GCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTC119940.1274650929605665No Hit
AAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAG119490.12698685974535676No Hit
CGGGGAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA118900.12635984286319288No Hit
CGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCG118100.1255096504805978No Hit
TGCTTTTTGATTCTTCGATGTCGGCTCTTCCTATCATACCGAAGCAGAAT117640.12502078986060564No Hit
CTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAG116140.12342667914323988No Hit
CACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGT116000.12327789547628575No Hit
CGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGT115420.12266150599890431No Hit
GCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTA115060.12227891942673653No Hit
TGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATC114680.12187507804500387No Hit
TGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACT114390.12156688330631316No Hit
AGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAA113950.12109927749588588No Hit
AAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCAC113810.12095049382893172No Hit
CAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAA112350.11939889273069572No Hit
GCTGGAAGAGGCCAGCACCTTTGCTGGCTCCGGTGCGCTTGTGACGGCCC111910.11893128692026843No Hit
CTCGACCCTTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGTCTTC111670.1186762292054899No Hit
CTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAA109520.11639133717726564No Hit
GTCTGATCAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAA109140.11598749579553298No Hit
GGTCTTGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTA107970.1147440894359877No Hit
GGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAAT107050.11376636819600335No Hit
CTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTTGTGGCGT106540.113224370552099No Hit
CGAACGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTAT106350.11302244986123265No Hit
CGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCC106120.11277801955123658No Hit
CCCGGAATTGGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAG106000.1126504906938473No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGA105920.1125654714555878No Hit
GTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATT104450.11100324295256936No Hit
TGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAATTATTGCTCTTCA104190.11072693042822597No Hit
CTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGGCGTGGAG103420.1099086202599782No Hit
CGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCT103410.10989799285519578No Hit
GGACGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATCAG101900.10829325473304756No Hit
CGGACTCTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGC100960.10729427868349836No Hit
GCTCAAGCGTGTTACCTATACTCTACCGTCAGGGTTGATATGATGCCCTG100810.10713486761176177No Hit
CGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGGGAATAA100680.10699671134959009No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATAC100560.10686918249220081No Hit
GCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGTC98920.10512628810788091No Hit
CAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAAAC98660.10484997558353751No Hit
GGTCAGTGACGAAGCCTAGACCGTAAGGTCGGGTCGAACGGCCTCTAGTG98520.10470119191658338No Hit
CTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTG97720.1038509995339883No Hit
AGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAAC97430.1035428047952976No Hit
TCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTG97290.10339402112834345No Hit
GAGGCCAGCACCTTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAA97270.10337276631877856No Hit
GCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGAAGCTCGTATCAGTT96420.10246943691227131No Hit
GGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCA96010.10203371331619132No Hit
CGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGTGAA95640.10164049933924112No Hit
CATGGTTTCAACGGGTAACGGGGAATAAGGGTTCGATTCCGGAGAGGGAG94550.10048211221795533No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)