FastQCFastQC Report
Thu 16 Sep 2021
H2HW2AFX3_n02_11220.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HW2AFX3_n02_11220.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8246480
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC853981.0355691155499074No Hit
ACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGG650390.7886880220409194No Hit
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGAT637630.7732147534463188No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG531030.6439474781967579No Hit
ACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT522170.6332035001600683No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA465850.5649076939494184No Hit
CTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTT411970.4995707259339743No Hit
CGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTCGG364260.4417157381088659No Hit
CAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTT359390.4358101881045003No Hit
CACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGA347990.4219861080121458No Hit
CCGGAATTGGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGC318380.3860798789301617No Hit
GCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATT277660.3367012349511549No Hit
GGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAAT262470.3182812545473948No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT254900.3091015803106295No Hit
CAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCT250820.3041540148038921No Hit
GGCTGGAAGAGGCCAGCACCTTTGCTGGCTCCGGTGCGCTTGTGACGGCC244230.2961627263996275No Hit
GCCGAAGTTTCCCTCAGGATAGCAGAAGCTCGTATCAGTTTTATGAGGTA244210.29613847362753565No Hit
TGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAACGAAAGTTAG232330.2817323270049767No Hit
GGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTT231810.2811017549305886No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGT230130.27906452207487314No Hit
TACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCG218680.26517981005228897No Hit
GCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTTG205740.24948826650886194No Hit
CGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAAT205400.24907596938330054No Hit
GGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGATAAGGGA197430.23941123970469824No Hit
CGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCT190660.231201676351607No Hit
ATCGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCC188310.22835197563081464No Hit
ACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCT187340.22717571618435986No Hit
CCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGCTGGCTCC184880.2241926252170623No Hit
CACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTG183370.22236154092412763No Hit
CGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGG181620.22023942336609073No Hit
CCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGA180180.21849322377547753No Hit
CGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTG179450.21760799759412502No Hit
AACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTC178460.2164074853755784No Hit
AAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAG177460.21519484677098594No Hit
AACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTC176740.21432174697567932No Hit
CTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAG176230.21370330128733717No Hit
TCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA172110.20870723023641602No Hit
CGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGT169920.20605155169235845No Hit
CCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGA166580.20200133875301948No Hit
CTTGGATTTATTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTT166200.2015405360832743No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGG166070.20138289306467727No Hit
GGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGC164840.1998913475810285No Hit
GCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGGTTCTGACGTGCAAATCG161220.19550159583240365No Hit
GCGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTC160160.19421619891153558No Hit
AGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA159830.19381602817202004No Hit
CCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATT150790.1828537751865038No Hit
CAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAAAC149120.18082866871683434No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTTTGACATTGTGAAGAGACATAG148780.18041637159127288No Hit
TGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAA147170.17846402343787895No Hit
CGAAGATGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTA145410.17632977949379613No Hit
CGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCT144520.1752505311357088No Hit
CGCTCAAGCGTGTTACCTATACTCTACCGTCAGGGTTGATATGATGCCCT144030.17465633821945847No Hit
GCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTA144020.17464421183341255No Hit
CTTCGGGATAAGGATTGGCTCTAAGGGTCGGGTAGTGAGGGCCTTGGTCA142390.17266761090792676No Hit
CTCGACCCTTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGTCTTC141200.1712245709684617No Hit
GCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGA137710.16699246223843384No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATAC132980.1612566816387113No Hit
CGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCG132490.16066248872246097No Hit
TGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCA132150.16025019159689952No Hit
CGCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGT129410.15692756182031609No Hit
CGGGGGCATCAGTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATTGA127760.15492670812273843No Hit
CCCGGAATTGGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAG127620.15475693871809548No Hit
CCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGG123130.14931219138347515No Hit
GCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGAAGCTCGTATCAGTT122560.1486209873788574No Hit
GCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAACGAAAGTTAGG122480.14852397629049No Hit
GCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGG119790.14526197844413619No Hit
GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCAC119510.14492243963485027No Hit
AAAAAATCAATGTCTTCGGACTCTTTGATGATTCATAATAACTTTTCGAA118870.14414635092791106No Hit
CGATCGTCGAATTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGT118870.14414635092791106No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGA115510.14007188521648026No Hit
CAAGAACGAAAGTTAGGGGATCGAAGATGATCAGATACCGTCGTAGTCTT115470.14002337967229656No Hit
CGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCT114630.13900476324443883No Hit
CGTGGGTTAGTCGATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTG113840.13804677874681076No Hit
GCCAGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTC113780.13797402043053522No Hit
TGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCTTTGGAAGAG113410.13752534414683598No Hit
GTCTGATCAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAA112270.13614293613760053No Hit
GGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCG112120.13596104034691164No Hit
CACGCTCATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCCGGAC110910.1344937476353547No Hit
CTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGG110610.13412995605397698No Hit
CTCAGGATAGCAGAAGCTCGTATCAGTTTTATGAGGTAAAGCGAATGATT108610.13170467884479198No Hit
CGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCC108110.1310983595424957No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG107650.13054054578438315No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA107560.13043140830996983No Hit
TGCTTTTTGATTCTTCGATGTCGGCTCTTCCTATCATACCGAAGCAGAAT107060.1298250890076736No Hit
CTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAA106650.12932790717979065No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG104860.12715728407757007No Hit
CGGACTCTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGC104820.12710877853338637No Hit
GCGAATGATTAGAGGTTCCGGGGTCGAAATGACCTTGACCTATTCTCAAA104540.12676923972410045No Hit
CTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATG103130.12505941929162503No Hit
CGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTAAGGTCGG103080.12499878736139541No Hit
GGACCATCGTAATGATTAATAGGGACGGTCGGGGGCATCAGTATTCAATT102820.12468350132420136No Hit
CACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGT102700.12453798469165027No Hit
CGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGCTGGCTCCG99310.12042713982208166No Hit
CAACTTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGG99280.12039076066394389No Hit
GGATTCTTCACGGTAACGTAACTGAATGTGGAGACGTCGGCGCGAGCCCT99080.12014823294302539No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGA98980.12002696908256613No Hit
AGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAA98080.11893559433843288No Hit
GCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTC96680.11723790029200337No Hit
CGACCCTTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGTCTTCGG96670.11722577390595745No Hit
CGTGGAGGTCAGTGACGAAGCCTAGACCGTAAGGTCGGGTCGAACGGCCT96240.11670433930598266No Hit
GCTCAAGCGTGTTACCTATACTCTACCGTCAGGGTTGATATGATGCCCTG96180.11663158098970713No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG95660.11600100891531902No Hit
GCTGGAAGAGGCCAGCACCTTTGCTGGCTCCGGTGCGCTTGTGACGGCCC94310.11436394679911915No Hit
TGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACT93840.11379400665496066No Hit
CGAAAGGGAATCCGGTTAAGATTCCGGAACCTGGATATGGATTCTTCACG93270.11310280265034293No Hit
AACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGT93170.11298153878988369No Hit
CGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGTGAA91420.1108594212318468No Hit
GCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGTTGA91280.11068965182720386No Hit
TCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTG91100.11047137687837719No Hit
CTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAA91060.11042287133419351No Hit
GTCGGCGCGAGCCCTGGGAGGAGTTATCTTTTCTTCTTAACAGCTTATCA90480.10971954094352984No Hit
GGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAAT90420.10964678262725429No Hit
AGTTTTCATGCCAGGTCGTACTGATAACCGCAGCAGGTCTCCAAGGTGAA90270.10946488683656543No Hit
GTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATT89800.10889494669240693No Hit
CGTAACTGAATGTGGAGACGTCGGCGCGAGCCCTGGGAGGAGTTATCTTT89770.10885856753426917No Hit
CGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCC89450.10847052318079956No Hit
GAGGCCAGCACCTTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAA89330.10832500654824846No Hit
AAAATCTCGACCCTTTGGAAGAGATGTATTTATTAGATAAAAAATCAATG88800.10768230808781444No Hit
GGCCAGCACCTTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATC88790.10767018170176851No Hit
AGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTCCGT87980.10668794443204857No Hit
GGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCA87810.10648179586926787No Hit
CTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGGCGTGGAG86650.10507513508794056No Hit
GAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCTATACTCTAC86630.1050508823158487No Hit
CGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTT86160.10448094217169024No Hit
CGAACGTAGAGTTAAGGTGCCGGAATACACGCTCATCAGACACCACAAAA86150.10446881578564429No Hit
AGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAAC85880.10414140336240432No Hit
GTTCCACGTCAACAGCAGTTGGACGTGGGTTAGTCGATCCTAAGAGATGG84960.10302577584617922No Hit
GCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGC84850.10289238559967405No Hit
CGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTTGCTCCAC84850.10289238559967405No Hit
CTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCT84400.1023466982276074No Hit
AGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGA83630.10141296650207118No Hit
GACGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATCAGA82890.10051561393467275No Hit
CTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTG82840.1004549820044431No Hit
CGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCC82700.10028521259980015No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)