FastQCFastQC Report
Thu 16 Sep 2021
H2HW2AFX3_n02_11219.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HW2AFX3_n02_11219.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7406911
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC1033101.3947784710792392No Hit
ACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGG523840.7072313951119434No Hit
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGAT477770.645032726868191No Hit
ACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT445300.6011952891022991No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG402790.543802942954222No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA355910.480510701424656No Hit
CTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTT350810.4736252399954583No Hit
CAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTT331400.44741998385021775No Hit
CGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTCGG311840.4210122141335301No Hit
CACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGA301780.4074303039418186No Hit
CCGGAATTGGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGC282120.3808875251774998No Hit
GGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAAT262470.354358247317944No Hit
CAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCT213270.2879337958833311No Hit
GCGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTC208610.2816423742637113No Hit
TGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAACGAAAGTTAG208260.2811698425970016No Hit
GCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATT196950.26590031930989855No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGT191210.2581507999758604No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT187370.25296645254681743No Hit
GGCTGGAAGAGGCCAGCACCTTTGCTGGCTCCGGTGCGCTTGTGACGGCC187150.2526694326420285No Hit
TACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCG184640.2492807055464822No Hit
CGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCT183340.24752558792727497No Hit
GCCGAAGTTTCCCTCAGGATAGCAGAAGCTCGTATCAGTTTTATGAGGTA178130.2404916165456828No Hit
AGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA177570.23973556587894737No Hit
GGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTT174510.23560428902142877No Hit
ATCGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCC173980.23488874106898272No Hit
CGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCT167250.2258026321633944No Hit
CCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGCTGGCTCC164540.22214388697258547No Hit
ACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCT164010.22142833902013942No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG163780.22111781821058737No Hit
CGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAAT161390.21789110197219866No Hit
CCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGA160610.2168380314006743No Hit
CGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGG158260.21366531878133813No Hit
AACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTC156820.21172118849544702No Hit
GCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTTG156670.211518674924No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG154500.20858897859040026No Hit
CGAAGATGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTA153980.2078869315427173No Hit
AACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTC152120.20537576325677467No Hit
CTTGGATTTATTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTT150790.20358014292327803No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149860.20232455878030664No Hit
GGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGATAAGGGA148260.20016441401820542No Hit
AAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAG146850.19826078644660372No Hit
GGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGC146350.19758574120844707No Hit
CGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGT142650.19259040644608796No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGATGG140200.18928268477912047No Hit
CGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTG138940.18758157077896576No Hit
CGGAGCTGGAATTCATTTTCCACGTTCTAGCATTCAAGGTCCCATTCGGG138820.18741955992180814No Hit
CTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAG136660.1845033644929715No Hit
TCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA133900.18077711477834688No Hit
CACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTG131830.1779824274923784No Hit
TGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCA131670.17776641301616827No Hit
CAAGAACGAAAGTTAGGGGATCGAAGATGATCAGATACCGTCGTAGTCTT128530.17352712892054462No Hit
TGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAA128160.17302759544430868No Hit
CGCTCAAGCGTGTTACCTATACTCTACCGTCAGGGTTGATATGATGCCCT125390.16928784482492093No Hit
CCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGA125120.16892332039631636No Hit
CCCGGAATTGGTTTATCCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAG122550.16545358787219125No Hit
GCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTA121980.1646840363006927No Hit
CCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATT119980.16198385534806614No Hit
CTTCGGGATAAGGATTGGCTCTAAGGGTCGGGTAGTGAGGGCCTTGGTCA119700.16160583001469844No Hit
GCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGA117690.15889214815730876No Hit
CAACTTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGG113390.1530867591091617No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTTTGACATTGTGAAGAGACATAG113260.15291124734724099No Hit
GGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAAT111890.1510616233946918No Hit
CTCGACCCTTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGTCTTC107310.14487820901317702No Hit
GCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAACGAAAGTTAGG107170.14468919634649316No Hit
CAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAAAC107090.1445811891083881No Hit
GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCAC106920.14435167372741484No Hit
CGGGGGCATCAGTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATTGA103320.1394913480126871No Hit
CGCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGT103110.13920782901266127No Hit
AAAAAATCAATGTCTTCGGACTCTTTGATGATTCATAATAACTTTTCGAA102730.13869479463166223No Hit
CGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCG102680.13862729010784658No Hit
CGTGGGTTAGTCGATCCTAAGAGATGGGGAAGCTCCGTTTCAAAGGCCTG101300.13676416525053428No Hit
CGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTAGACCGTAAGGTCGG99190.13391547434551326No Hit
CTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGG98500.1329839119168571No Hit
GGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCG97120.1311207870595448No Hit
CCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGG96970.1309182734880978No Hit
CACGCTCATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCCGGAC96370.13010821920230983No Hit
GTCTGATCAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAA94230.12721902558299944No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATAC93490.12621995863052762No Hit
GCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGAAGCTCGTATCAGTT93380.12607144867813316No Hit
CGGACTCTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGC91940.12412731839224204No Hit
GCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGG91430.12343877224932227No Hit
CTTGTGCTGGCGATGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATG90700.12245320620161361No Hit
CGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGTCT90670.1224127034873242No Hit
GCCAGCGAGTCTAACCTTGGCCGAGAGGTCTTGGTAATCTTGTGAAACTC90600.12231819715398229No Hit
GCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGGTTCTGACGTGCAAATCG89740.12115711934435286No Hit
CAGATGTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAA89600.12096810667766901No Hit
CGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGTGAA89270.12052257682048562No Hit
TGGTAATTCTAGAGCTAATACATGCTTAAAATCTCGACCCTTTGGAAGAG86590.11690433434396606No Hit
CGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCC86090.11622928910580942No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGA85990.11609428005817811No Hit
CGAAAGGGAATCCGGTTAAGATTCCGGAACCTGGATATGGATTCTTCACG84590.11420415339133952No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTTGTGCTGGCGA84120.11356961086747229No Hit
TGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAA84020.11343460181984097No Hit
CGGCGCCAGTGAAATACCACTACCTTTATAGTTTCTTTACTTATTCAATG83190.11231402672450094No Hit
CACGCGCGCTACACTGACGGAGCCAGCGAGTCTAACCTTGGCCGAGAGGT82890.11190899958160695No Hit
CGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGCTGGCTCCG82730.11169298510539684No Hit
CTCAGGATAGCAGAAGCTCGTATCAGTTTTATGAGGTAAAGCGAATGATT81670.11026188920050478No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA80380.10852027248606066No Hit
TGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACT80060.10808824353364041No Hit
GCGAATGATTAGAGGTTCCGGGGTCGAAATGACCTTGACCTATTCTCAAA79130.10683265939066906No Hit
GCTCAAGCGTGTTACCTATACTCTACCGTCAGGGTTGATATGATGCCCTG78820.10641413134301195No Hit
CGACCCTTTGGAAGAGATGTATTTATTAGATAAAAAATCAATGTCTTCGG78560.10606310781917051No Hit
GCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGC77690.10488852910477794No Hit
AACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGT77670.10486152729525168No Hit
CTCTACCGTCAGGGTTGATATGATGCCCTGACGAGTAGGCAGGCGTGGAG77370.10445650015235772No Hit
GAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAA77330.10440249653330518No Hit
GTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATT77280.10433499200948951No Hit
GGACCATCGTAATGATTAATAGGGACGGTCGGGGGCATCAGTATTCAATT76930.10386246034277988No Hit
CTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAA76840.10374095219991168No Hit
CGTGGAGGTCAGTGACGAAGCCTAGACCGTAAGGTCGGGTCGAACGGCCT76740.10360594315228036No Hit
AGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAA76600.10341693048559648No Hit
CGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCC75170.1014863011044685No Hit
GGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTC74870.10108127396157454No Hit
CTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAA74090.10002820339005018No Hit
AAAATCTCGACCCTTTGGAAGAGATGTATTTATTAGATAAAAAATCAATG74070.10000120158052393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)