FastQCFastQC Report
Thu 16 Sep 2021
H2HW2AFX3_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HW2AFX3_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6838293
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAG338100.4944216341709839No Hit
CCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTA197520.28884401414212585No Hit
CCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTT195930.28651887247299873No Hit
CCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGG154050.2252755183201422No Hit
CCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA151840.22204371763538067No Hit
CTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTC141670.2071715850724735No Hit
CAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCAC139440.2039105373226915No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC137970.2017608780436872No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT137150.20056174837784813No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGC131620.19247493489968914No Hit
CTTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAG120830.17669614332114755No Hit
TCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC120190.1757602372404926No Hit
CCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATT119230.17435637811951024No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC119140.17422476632691813No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACG113770.16637192936892292No Hit
CCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAA112990.16523129383312474No Hit
CTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTA102560.14997894942495152No Hit
CCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCA97140.1420529948044051No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA96090.14051752389083066No Hit
CTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCT95760.14003494731799296No Hit
TTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGA94880.13874807645709245No Hit
CCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAAT87990.12867246255754178No Hit
CGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTA87700.12824838011474501No Hit
CGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCT80800.11815814268268411No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTA80240.11733922486211106No Hit
CACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTT77320.11306915336912297No Hit
CCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGACCT76650.11208937669093734No Hit
GCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTT76470.11182615310575314No Hit
GTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACT75660.11064164697242426No Hit
GTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCA75110.10983735268436143No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACT74450.10887219953868603No Hit
CTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATAA73940.10812639938066414No Hit
CCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC73710.10779005813292877No Hit
TACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGC73200.10704425797490688No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)