FastQCFastQC Report
Thu 16 Sep 2021
H2HW2AFX3_n01_11542.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HW2AFX3_n01_11542.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9858746
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTA661130.6706025289626084No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGC616230.6250592113844905No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC605610.6142870503003119No Hit
CTTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAG582750.5910995171191143No Hit
CCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGG549180.5570485333530248No Hit
CCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA547090.5549285882809031No Hit
CCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATT517130.5245393278212057No Hit
CCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTT512280.5196198380605404No Hit
CCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAA448460.4548854387768992No Hit
TCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC417020.42299497319435964No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACG415930.421889355907942No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT415780.4217372067400864No Hit
GCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTT402190.4079524921323665No Hit
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAG400900.4066440092888081No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC392270.397890360498181No Hit
GTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCA381440.3869051905790047No Hit
CCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCA344580.3495170683979484No Hit
CTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTC335540.34034754521518257No Hit
CCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAA334640.33943465020804875No Hit
CCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAAT329250.3339674234431032No Hit
CACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTT328460.3331661044923969No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTA322990.327617731504595No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACT319560.3241385871996297No Hit
ACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA310400.3148473446825793No Hit
CCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAA301240.305556102165529No Hit
CGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCT298900.3031825751469812No Hit
GCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCC298600.3028782768112699No Hit
CCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGACCT296610.3008597645177186No Hit
CCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGA295330.2995614249520172No Hit
CCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTATTCTA294970.2991962669491637No Hit
GCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTATT293340.2975429126584659No Hit
GTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACT291840.29602142097990963No Hit
CTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTA290940.2951085259727758No Hit
GCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGG287760.29188296361423655No Hit
CTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCT270490.2743655227551252No Hit
CTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATAA262620.2663827630816333No Hit
CCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGAA261950.2657031634652115No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG261490.2652365726837876No Hit
CCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC257490.2611792615409708No Hit
CCTTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAA252750.256371347836733No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTG247290.2508331181267881No Hit
TTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGA247150.2506911122367895No Hit
CCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATA244850.2483581583296699No Hit
CCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTC244410.2479118541039601No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA238720.24214032900330326No Hit
CCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAATA232460.23579063706479506No Hit
CCCAGCACGACGGAGTTTCACAAGATTACCAAGACCTCTCGGCCAAGGTT230390.23369097854838738No Hit
GCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCC227600.23086100402627271No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT226170.22941051529271572No Hit
CCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA226100.22933951234771643No Hit
CCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAG223280.22647910799203064No Hit
CCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCC217300.2204134278335196No Hit
CCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACT216880.21998741016352386No Hit
TCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCT216600.21970339838352668No Hit
GCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGT212960.2160112452435634No Hit
CTCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGT209460.21246109799359877No Hit
GTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTAC208320.211304764317896No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGC206770.2097325562500545No Hit
CTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACTA200290.20315971219869142No Hit
GCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA199220.20207438146798792No Hit
TTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAGG198910.2017599398544196No Hit
GCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGAT192930.1956942596959086No Hit
CCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGT192810.19557254036162408No Hit
CCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGT190210.19293528811879318No Hit
CCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCAT189310.19202239311165945No Hit
CTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAAC188590.19129207710595242No Hit
CCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA187890.19058204765595949No Hit
GCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC187180.18986187492810952No Hit
CCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCG186590.18926342153454406No Hit
CCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGT179900.18247756864818304No Hit
CGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTA178930.18149367069604996No Hit
GCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCC175770.17828839489322476No Hit
CCCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGATCGGT173120.17560042626110867No Hit
GCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTC171930.1743933761961207No Hit
CCCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGA170920.17336890513255945No Hit
ACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTT169690.1721212819561433No Hit
CCCTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAA168600.17101566466972573No Hit
CCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAG168600.17101566466972573No Hit
CCCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAG166340.1687232838740343No Hit
CCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTT166290.1686725674847491No Hit
GCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGCAGTCCTCA165290.1676582396990449No Hit
ACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAA163470.16581216312906327No Hit
GTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCAC162210.164534110119076No Hit
CCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTC161670.16398637311479575No Hit
CCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGT161020.163327060054088No Hit
CCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTTC159440.1617244221526754No Hit
GCGGTAACATTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA156130.15836699718199454No Hit
CGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTAT156120.1583568539041375No Hit
CCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGCC152670.15485742304345806No Hit
CTCCTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTG152150.1543299725948919No Hit
CCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCG150520.15267661830419407No Hit
ACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTT150400.15255489896990956No Hit
TTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTT149310.15144928168349198No Hit
CTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTATC148430.15055667323207234No Hit
TCCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAG147780.1498973601713646No Hit
CGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTATTT146860.14896417860851674No Hit
CGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTA142690.1447344317421303No Hit
TCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTC142160.14419683801570707No Hit
TTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGC141790.1438215367349965No Hit
GCTACTACCACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGG140370.14238119127929658No Hit
CCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTT140260.14226961522286913No Hit
CCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGAC138430.14041339537503045No Hit
GCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC137530.13950050036789668No Hit
CGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCC136770.1387296112507615No Hit
CCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCGC136710.13866875158361924No Hit
CTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTGAGCT136620.13857746208290586No Hit
GCCGACTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTT136080.1380297250786256No Hit
CTCTTACTCAAATCCATCCGAAGACATCAGGATCGGTCGATTGTGCACCT135630.13757327757505874No Hit
CATAAAACTGATACGAGCTTCTGCTATCCTGAGGGAAACTTCGGCAGGAA135500.1374414149629172No Hit
CTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAG135230.13716754646077706No Hit
GCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAA135070.1370052540150644No Hit
CCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAAT132720.13462158371865954No Hit
GTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAGGTGCTG131330.13321166809653073No Hit
GCGTGTATTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCA131020.13289722648296245No Hit
CCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTAAAAGCTCTTCA130380.13224805670011175No Hit
CTCCACATTCAGTTACGTTACCGTGAAGAATCCATATCCAGGTTCCGGAA129770.1316293167508322No Hit
CTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTTCA129750.13160903019511813No Hit
TCCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACC128390.13022954440656043No Hit
GCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGA127620.1294485120115682No Hit
CCTCACTACCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCT127570.129397795622283No Hit
CCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAA127320.12914421367585696No Hit
GCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAA126810.12862690650514783No Hit
TCCCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGG126140.12794730688872602No Hit
TCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA125150.12694312238087888No Hit
CTCACTACCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTA124980.12677068665730915No Hit
GCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGC124920.12670982699016692No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC123620.12539120086875147TruSeq Adapter, Index 2 (100% over 50bp)
CCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAATG123560.1253303412016092No Hit
GCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCA121740.1234842646316276No Hit
CCTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAA121630.12337268857520013No Hit
CTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGTT119930.12164833133950302No Hit
GGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAA114990.11663755207812433No Hit
CGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACG112120.11372643133315333No Hit
CGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATT111740.11334098677458573No Hit
CCCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTC111650.11324969727387235No Hit
CCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCC111520.11311783466173081No Hit
CCCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATA110320.1119006413188858No Hit
CCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTG110310.11189049804102874No Hit
TGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAG109860.11143405053746186No Hit
TGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATT109270.11083559714389639No Hit
CGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCA108730.11028786013961613No Hit
CCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGC108090.10963869035676545No Hit
CAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCAC107610.10915181301962744No Hit
TGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT107560.10910109663034223No Hit
CACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCT106010.10752888856250076No Hit
CCCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACC105520.1070318679475057No Hit
CCCTCACTACCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATC104210.1057030985482332No Hit
CTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGA104100.10559152249180577No Hit
TGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT102720.10419175014753398No Hit
TTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCC102220.1036845862546819No Hit
GCGCCCGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC101640.10309627613897346No Hit
ACTCATTCCAATTACAAGACCCGAATGGGCCCTGTATCGTTATTTATTGT101180.10262968535754953No Hit
ACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGAC100820.10226452735469602No Hit
CCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAGAA99160.10058074323042708No Hit
CTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGG99020.10043873734042849No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)