FastQCFastQC Report
Thu 16 Sep 2021
H2HW2AFX3_n01_11541.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HW2AFX3_n01_11541.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10197783
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAG1234871.2109200597816212No Hit
CCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTT1058011.0374902074303798No Hit
CCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTA929200.9111784394706182No Hit
CTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTC866950.8501357598999704No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC838270.8220120000592286No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT833700.8175306338642429No Hit
CCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGG776610.7615478776122222No Hit
CCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA698660.6851096949209451No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC695440.6819521458732747No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGC673060.6600061993866706No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA646900.634353564887584No Hit
TCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC640130.6277148670451215No Hit
CTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTA607540.5957569405036369No Hit
CCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAA607130.5953548923329708No Hit
CTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCT583190.5718792015872469No Hit
CAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCAC571650.5605630164909373No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACG545380.5348025154094768No Hit
CCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAAT541080.530585912643954No Hit
TTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGA537210.5267909701549837No Hit
CGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTA520760.5106600130636237No Hit
CCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATT513460.5035015944151783No Hit
CCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCA469290.46018825856561174No Hit
CCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGACCT465920.4568836187237951No Hit
GTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACT406460.39857682792426546No Hit
GTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCA391380.38378930008610695No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTA389520.3819653742386948No Hit
CTTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAG388620.38108282947381794No Hit
CACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTT386720.3792196794146335No Hit
CTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATAA375000.36772698536534854No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG358760.3518019553857932No Hit
CGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCT356880.3499584174324949No Hit
GTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCAC346400.3396816739481513No Hit
TTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTT345330.33863242628324214No Hit
CCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC343420.3367594701711146No Hit
TCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCT336640.33011096627570913No Hit
CTCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGT335060.32856160991070316No Hit
TACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGC334170.3276888711987694No Hit
CCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACT329210.3228250689390037No Hit
GTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTAC327900.32154047600346075No Hit
CTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTATC320180.3139702031314061No Hit
CTCCTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTG317560.3114010172603202No Hit
CCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGT313620.30753743240074827No Hit
CCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTATTCTA313390.30731189318305757No Hit
CCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGAA313350.30727266897128525No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACT305930.29999657768752286No Hit
CCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCC303620.2977313794576723No Hit
GCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTT297270.2915045358388191No Hit
GCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAA297110.2913476389917299No Hit
ACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCA290150.284522626143349No Hit
CCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA288560.28296346372539993No Hit
CCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAATA267560.2623707525449404No Hit
GCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCC260740.25568302443776264No Hit
CCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGAC256440.2514664216722399No Hit
TTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGC248120.24330778562360075No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTG240870.23619839723987066No Hit
GCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGG235200.23063836522114659No Hit
GCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTATT229020.22457822450232565No Hit
CCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTC223090.21876323510708162No Hit
CTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTTCA221140.21685105478318178No Hit
GCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCA219190.214938874459282No Hit
CCTTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAA218800.21455643839450203No Hit
CCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATA218590.21435051128269744No Hit
CTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGA213610.2094670969170456No Hit
CCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAA207840.20380900436889077No Hit
TGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAA207510.20348540462176926No Hit
CTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAAC206610.20260285985689241No Hit
CCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTT205500.201514387980211No Hit
GCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC202180.1982587784031098No Hit
CCCTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAA198460.1946109267082855No Hit
GCCGACTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTT195660.19186523188422425No Hit
AAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACC192550.18881554941892761No Hit
CCCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGA192050.1883252467717738No Hit
TCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTC191260.18755056858927083No Hit
GCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC190040.18635423013021557No Hit
TGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT189620.18594237590660637No Hit
CTCACTACCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTA188100.18445185585925883No Hit
CGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTA186650.18302997818251282No Hit
CCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTTC186230.18261812395890362No Hit
CCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAG184490.1809118707468084No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGC183980.18041176204671153No Hit
GCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCC181790.1782642364521779No Hit
ACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAA180750.177244406946098No Hit
ACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTT178840.17537145083397046No Hit
GCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGT178430.17496940266330435No Hit
CCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCAT178420.17495959661036128No Hit
GGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAA174620.17123329649199243No Hit
GCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCC174190.17081163621544015No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG172070.16873275299150806No Hit
CCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGT170540.16723242689121745No Hit
CCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAATG169680.16638910633811288No Hit
CCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGA168880.16560462210266683No Hit
CTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGACAT168010.16475149549661922No Hit
ACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAG167970.16471227128484692No Hit
GCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTC165710.16249610331971173No Hit
CCTCACTACCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCT164380.16119189827828265No Hit
GCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGC163250.16008381429571505No Hit
TGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT161720.15858348819542445No Hit
CCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTAAAAGCTCTTCA161140.15801473712472602No Hit
CCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAA158600.15552399967718472No Hit
CCCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATA158280.1552102059830063No Hit
CGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCC157950.1548866062358848No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT156770.15372949198860184No Hit
CCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCAT153810.15082690031745136No Hit
GCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGAT153800.15081709426450826No Hit
TCCCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGG153480.15050330057032985No Hit
CCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCG152660.14969920422899763No Hit
CTCCACATTCAGTTACGTTACCGTGAAGAATCCATATCCAGGTTCCGGAA152070.14912064710535614No Hit
CCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCCG149670.1467671943990179No Hit
CGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTAT148530.14564930436350723No Hit
CCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAG147670.14480598381040272No Hit
CGCGTCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT146950.14409994799850123No Hit
ACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTT142320.13955974548585706No Hit
TTCCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGAC141270.1385301099268341No Hit
CCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCGC140440.13771620753255878No Hit
ATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCA138350.13566674246745591No Hit
CACTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGC136930.13427428294953914No Hit
CTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCGCA136750.13409777399656378No Hit
TGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAG136620.1339702953083038No Hit
CCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGT133840.13124421259012867No Hit
CGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGT133110.13052837072528412No Hit
TCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTT133110.13052837072528412No Hit
GTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTATACCAT132690.1301165165016749No Hit
CTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGA132410.1298419470192688No Hit
CTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAG131450.1289005659367335No Hit
GCGGTAACATTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA129820.12730217930701213No Hit
CCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTTC128840.1263411861185907No Hit
CCCTCACTACCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATC128830.12633138006564762No Hit
CCTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAA128310.12582146531260766No Hit
GCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTT127980.12549786556548614No Hit
CCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAATGA127420.12494872660067388No Hit
CCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGCC127210.1247427994888693No Hit
CGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCA126690.12423288473582936No Hit
CTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCA126420.1239681213063663No Hit
GCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAA125600.12316402496503408No Hit
GCGCCCGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC125600.12316402496503408No Hit
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC121840.1194769490584375No Hit
CAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAG119400.11708427214032696No Hit
TCCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACC118960.11665280581083164No Hit
CGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT118120.11582909736361324No Hit
CCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCG115960.11371098992790883No Hit
CCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAGAA113260.11106335563327835No Hit
CTTCCCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGT113110.1109162648391322No Hit
GCGTGTATTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCA112280.11010236244485688No Hit
CCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTT111890.10971992638007692No Hit
CTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCC111860.10969050822124768No Hit
TTTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTG111590.10942574479178464No Hit
GTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCA110690.1085432000269078No Hit
CCTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAA110670.10852358792102165No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT110180.10804309132681095No Hit
CTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC108850.10673888628538183No Hit
GCGCTTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC107120.1050424391262297No Hit
TCCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGACC106950.1048757362261974No Hit
CGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACG106890.10481689990853894No Hit
TGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCA106850.10477767569676663No Hit
CGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT105220.10317928906704527No Hit
TTGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAAT104650.10262034404928994No Hit
CTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTT104260.10223790798450996No Hit
CCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTC103880.10186527797267307No Hit
CCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC103790.10177702349618539No Hit
CCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCC102700.10070816372539011No Hit
CCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAAT102550.10056107293124397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)