FastQCFastQC Report
Thu 16 Sep 2021
H2HW2AFX3_n01_11538.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HW2AFX3_n01_11538.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8995822
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAG2046532.274978317712378No Hit
CAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCAC947631.0534112391285644No Hit
CCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTT913821.0158271250809543No Hit
CTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTC864620.9611350691465438No Hit
CTTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAG850300.9452165683136015No Hit
CCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGG766920.8525290962849198No Hit
CCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTA641190.7127642143208258No Hit
TCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC572690.6366177543308438No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACG544160.6049030316518046No Hit
TTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGA517430.5751892378484145No Hit
CCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA493330.5483990234577786No Hit
CTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTA486540.5408510750879686No Hit
CGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTA476710.529923780172618No Hit
CCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAA421930.46902884472369505No Hit
TACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGC398840.4433613737577289No Hit
CCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAA384600.42753180309703775No Hit
GTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCAC382850.4255864555790455No Hit
CTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCT382850.4255864555790455No Hit
CCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC382340.42501952573094487No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT381910.4245415260550954No Hit
ACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCA374980.4168379498838461No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC374130.4158930668036784No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA365860.40669991024722363No Hit
CCTTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAA361770.4021533551908875No Hit
CGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCT352720.3920931294549848No Hit
CCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATT346610.385301087549309No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTA345990.3846118787143632No Hit
CCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTATTCTA344870.38336685630284817No Hit
GTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACT343430.3817661132023288No Hit
GTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCA334920.3723061661291208No Hit
CTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATAA321840.35776608296607026No Hit
CTCCTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTG306030.3401912576749518No Hit
CCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAAT305190.3392574908663155No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGC303110.3369453063877876No Hit
AAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACC299890.33336586695468184No Hit
CCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGT299660.33311019270946No Hit
GTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTAC295170.32811898679186846No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC293830.326629406406663No Hit
CACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTT293430.32618475554540766No Hit
CCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGACCT289710.32204950253573267No Hit
CTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTATC265720.2953815671319419No Hit
CCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCA265110.29470347456852747No Hit
TCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCT262090.2913463605660494No Hit
TTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTT246540.27406055833474696No Hit
TTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGC237000.2634556352938064No Hit
CCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGAA236970.26342228647921223No Hit
CCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACT227610.25301745632583655No Hit
CTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAAC218930.24336853263659508No Hit
TTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAGG214580.23853295452044293No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACT213620.23746579245343002No Hit
CCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGAC211660.23528700323327875No Hit
CTCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGT207040.2301512857857792No Hit
GCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCC205420.22835044979769495No Hit
GCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTT204240.22703872975699163No Hit
CCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA200360.22272561640281457No Hit
CCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCC199950.2222698492700278No Hit
GCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAA193480.21507762158922222No Hit
GCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC190870.21217627471953093No Hit
GTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCA188210.2092193464921827No Hit
CCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATA187240.20814106815363842No Hit
CTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTTCA185890.20664037149690154No Hit
ATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCA179940.20002618993572793No Hit
GCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCA179900.19998172484960242No Hit
ACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTT175910.19554633250858008No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG173830.19323414803005218No Hit
ACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA166460.185041455911422No Hit
CCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCAT163430.18167322563741256No Hit
TTTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTG161670.17971676184788893No Hit
CTCACTACCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTA161370.1793832737019474No Hit
CTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGA159890.17773806551530255No Hit
CTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAG152770.16982328018495696No Hit
CCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAATA152690.1697343500127059No Hit
CCCTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAA151350.1682447696275004No Hit
CTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGACAT151310.16820030454137488No Hit
CTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCA149990.16673295669923216No Hit
CCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTC147400.16385384237260364No Hit
CCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTTC144820.1609858443175065No Hit
CCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTTC144160.16025217039643513No Hit
CCTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAA143140.15911831070023397No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTG142150.15801779981862693No Hit
CTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCGCA139160.15469403463074302No Hit
CCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTAAAAGCTCTTCA138860.15436054648480152No Hit
CGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCA138650.15412710478264244No Hit
CCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCGC138460.15391589562354613No Hit
CCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTT137830.15321557051706894No Hit
GTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAGGTGCTG137080.1523818501522151No Hit
ACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAG136480.15171487386033206No Hit
CCCAGCACGACGGAGTTTCACAAGATTACCAAGACCTCTCGGCCAAGGTT135190.1502808748327835No Hit
CCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGT133150.1480131554403811No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGC132800.14762408593678264No Hit
CTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGA132010.1467459004858033No Hit
CCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAA129620.14408911158980245No Hit
CCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCG129010.14341101902638803No Hit
GCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTATT128360.14268846137684804No Hit
CCCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGA128290.14261064747612837No Hit
CTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGG127990.14227715933018684No Hit
GCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGC126100.1401761840107552No Hit
CTCCACATTCAGTTACGTTACCGTGAAGAATCCATATCCAGGTTCCGGAA125200.13917571957293065No Hit
CGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCC125150.13912013821527372No Hit
GCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCC124540.13844204565185927No Hit
CCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA123120.13686353509440272No Hit
GGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAA122990.13671902356449472No Hit
CCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCG122930.1366523259353064No Hit
GCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGAT122730.13643000050467874No Hit
GCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTC122150.13578525675585845No Hit
CCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAGAA121980.13559628013982492No Hit
CTCTTACTCAAATCCATCCGAAGACATCAGGATCGGTCGATTGTGCACCT118950.1322280498658155No Hit
GCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCC118190.13138321322943028No Hit
CCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTT117040.1301048420033211No Hit
CCTCACTACCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCT112980.1255916357615791No Hit
CCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCAT112400.12494689201275881No Hit
TGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAA112220.1247467991251939No Hit
CCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAG109660.12190103361315954No Hit
CTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTAC109230.12142303393731001No Hit
CGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTAT108730.1208672203607408No Hit
TCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTC108080.12014466271120083No Hit
CTGCCTACTCGTCGGGGCATCATATCAACCCTGACGGTAGAGTATAGGTA107250.11922201217409592No Hit
CCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAATG107230.11919977963103316No Hit
GCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA106740.11865508232599532No Hit
CCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGCC106620.11852168706761872No Hit
CCCGCTGATTCTGCCAAGCCCATTCCCTTGGCTGTGGTTTCGCTAGATAG106500.11838829180924211No Hit
ACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTT106420.11829936163699105No Hit
CGCTGATTCTGCCAAGCCCATTCCCTTGGCTGTGGTTTCGCTAGATAGTA106100.11794364094798673No Hit
TCCCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGG105440.11720996702691538No Hit
CTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTG104640.11632066530440466No Hit
GCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGT102470.11390843438209428No Hit
CACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCT102390.1138195042098432No Hit
CGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTATTT101260.11256336552679677No Hit
CTTCCCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGT100230.11141838955906419No Hit
TCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTT100180.11136280820140729No Hit
CGCCTGCCTACTCGTCGGGGCATCATATCAACCCTGACGGTAGAGTATAG99200.11027341359133161No Hit
CCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCCG97490.10837253115946492No Hit
CTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC97350.10821690335802553No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATT97060.10789453148361539No Hit
CTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTT96230.1069718809465105No Hit
TTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCC96120.10684960195966528No Hit
CACTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGC96060.10678290433047696No Hit
CCGCTGATTCTGCCAAGCCCATTCCCTTGGCTGTGGTTTCGCTAGATAGT96020.10673843924435143No Hit
TCCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAG94600.10515992868689487No Hit
TTCCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGAC93560.1040038364476309No Hit
GCGTGTATTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCA93560.1040038364476309No Hit
CATAAAACTGATACGAGCTTCTGCTATCCTGAGGGAAACTTCGGCAGGAA92520.10284774420836694No Hit
CTGCTATCCTGAGGGAAACTTCGGCAGGAACCAGCTACTAGATGGTTCGA92090.10236974453251743No Hit
GCCGACTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTT91400.10160272179685192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)