FastQCFastQC Report
Thu 16 Sep 2021
H2HW2AFX3_n01_11537.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HW2AFX3_n01_11537.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10814961
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTA818670.7569791513811285No Hit
CTTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAG789110.72964664412567No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGC707510.6541956092120905No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC705560.6523925513924645No Hit
CCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGG655310.6059291383482567No Hit
CCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTT619860.573150471832492No Hit
CCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATT597170.5521702759723313No Hit
CCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA558700.5165991814487357No Hit
GCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTT535600.49523988112393563No Hit
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAG520890.48163835264870586No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT517860.47883667819051773No Hit
TCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC483370.4469456709090306No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC482290.4459470542704685TruSeq Adapter, Index 9 (100% over 50bp)
CCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACG478370.4423224457305024No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC442300.4089704992926003No Hit
CTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTC440300.40712120922118905No Hit
GTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCA434830.4020634008758793No Hit
GTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACT427870.39562787142736805No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTG422640.39079197789062764No Hit
CACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTT419880.3882399575920801No Hit
GCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTATT408290.3775233216282518No Hit
CCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAA407710.3769870275075426No Hit
CGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCT407290.3765986765925462No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTA400920.37070868771510135No Hit
CCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCA393380.36373686414588086No Hit
CCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAA386230.3571256521405856No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACT374610.3463812768256862No Hit
CCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTATTCTA361950.33467527067365294No Hit
CTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATAA354010.3273335890901502No Hit
GCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGAT352150.3256137493237377No Hit
GCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGG340950.3152577249238347No Hit
CCTTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAA340000.3143793121399143No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGC336760.3113834622242281No Hit
CTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTA333440.30831364070568534No Hit
GCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCC325480.30095346622146857No Hit
CCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGA322210.2979298769547112No Hit
CCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATA308280.28504957160733174No Hit
CCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC306310.28322802088699167No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG305750.2827102196669965No Hit
TTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGA305230.28222940424842957No Hit
CCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGAA299420.27685721659097984No Hit
CCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAAT298050.27559045289206313No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA291840.26984840722033115No Hit
ACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA290000.2681470603546328No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT283020.2616930380054075No Hit
CCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGACCT280440.25930745381328696No Hit
CTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCT278140.25718077023116404No Hit
GCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCC272330.25180858257371436No Hit
CTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAAC271750.25127228845300503No Hit
CTCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGT271130.2506990085308676No Hit
CCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAA268780.24852609269695936No Hit
GTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTAC267210.2470743999909015No Hit
CCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACT264900.2449384699584215No Hit
TTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAGG253040.2339721798349527No Hit
TCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCT249750.2309300976674812No Hit
CCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA248310.2295986088160651No Hit
CCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAG233400.21581215133369414No Hit
CCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGT230990.21358375679764358No Hit
CCCAGCACGACGGAGTTTCACAAGATTACCAAGACCTCTCGGCCAAGGTT226360.20930265028232647No Hit
CGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTA225180.20821156914019387No Hit
GCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTC213960.19783705183957664No Hit
GCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCC211950.19597851531780835No Hit
CCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCAT210460.19460079421460696No Hit
CTCCTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTG208300.19260356093748282No Hit
GCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGT207810.19215048486998704No Hit
CCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTC206170.19063406701142982No Hit
CCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCC205730.19022722319571936No Hit
CTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACTA205690.1901902373942911No Hit
GCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC204560.18914538850394375No Hit
CCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAG202800.18751801324110184No Hit
CCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGT201360.18618652438968572No Hit
CCCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGATCGGT200590.18547454771219238No Hit
ACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTT200100.18502147164469665No Hit
CCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAATA195330.18061091482438077No Hit
CCCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGA194390.1797417484908175No Hit
GTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCAC194120.17949209433117697No Hit
GGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAA192510.1780034158236909No Hit
CCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGCC191310.17689384178084416No Hit
GTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAGGTGCTG190620.17625583670620726No Hit
CGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTATTT190510.17615412575227965No Hit
CCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGT189920.17560858518121333No Hit
CCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCG186650.17258499591445592No Hit
CTCTTACTCAAATCCATCCGAAGACATCAGGATCGGTCGATTGTGCACCT186380.1723353417548154No Hit
GCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA186300.17226137015195894No Hit
CTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAG184400.17050454458411823No Hit
CCCTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAA184070.17019941172233538No Hit
CGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTAT181560.16787855268271426No Hit
GCTACTACCACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGG180810.16718506890593504No Hit
ACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTT179550.16602001616094592No Hit
GCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGC177510.16413374028810646No Hit
GCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC175230.1620255496066976No Hit
CCCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAG175000.1618128812484853No Hit
CCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA172330.15934407900315128No Hit
CTCCACATTCAGTTACGTTACCGTGAAGAATCCATATCCAGGTTCCGGAA168960.1562280252328233No Hit
CGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTA167670.15503523313676304No Hit
TTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGC167170.15457291061891024No Hit
TCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTC166830.15425853130677034No Hit
TCCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAG166210.15368525138463285No Hit
CCTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAA164820.152399994785002No Hit
CGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATT164160.15178972906143628No Hit
CTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTATC164150.1517804826110792No Hit
CCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGT160930.14880312559610712No Hit
CTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTGAGCT160540.1484425140321819No Hit
GCGGTAACATTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA160150.1480819024682567No Hit
TTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTT157650.14577028987899263No Hit
CCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTTC156550.14475318033971643No Hit
GCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGA155740.1440042178607949No Hit
CCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTT154350.14271896126116404No Hit
GCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGCAGTCCTCA153630.142053216835456No Hit
ACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAA153480.14191452008010016No Hit
CCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTC153360.14180356267581548No Hit
GGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTT150610.139260788827625No Hit
TGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATT148940.13771663161799658No Hit
CCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTAAAAGCTCTTCA147110.13602453120265529No Hit
TCCCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGG146080.13507214681587848No Hit
CCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTT145780.1347947533051668No Hit
CTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTTCA144630.1337314115141053No Hit
CTCACTACCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTA140940.13031947133235156No Hit
CGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCC139610.12908969343486304No Hit
GCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCA139580.1290619540837919No Hit
GCCGACTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTT138710.128257512902728No Hit
GCGTGTATTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCA138590.1281465554984433No Hit
CCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAA138320.1278969013388028No Hit
CATAAAACTGATACGAGCTTCTGCTATCCTGAGGGAAACTTCGGCAGGAA138110.1277027258813046No Hit
CCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCG137240.1268982847002407No Hit
AGCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGC136400.126121582870248No Hit
CCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTG135670.12544659199418287No Hit
CCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCGC134820.12466064371383309No Hit
CGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACG132770.12276512139063654No Hit
CGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCA132410.12243224917778252No Hit
TCCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACC130930.12106377452493819No Hit
ACTCATTCCAATTACAAGACCCGAATGGGCCCTGTATCGTTATTTATTGT130510.12067542360994182No Hit
CCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGC130430.12060145200708536No Hit
TGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT129980.12018536174101785No Hit
CCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAGAA129730.11995420048209143No Hit
CAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCAC128100.11844702907389125No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCT127490.11788299560211081No Hit
CTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGG124360.11498885664035219No Hit
CTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGTT123480.11417516900893124No Hit
GCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAA121900.11271422985251633No Hit
CCTCACTACCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCT121800.11262176534894577No Hit
TGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT120240.111179319093245No Hit
GCGCCCGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC119270.11028241340861053No Hit
ACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAG115720.10699992353185556No Hit
TCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA114460.10583487078686644No Hit
CTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGA114330.10571466693222473No Hit
CACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCT111460.10306093567974957No Hit
CACTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGC111440.10304244277903545No Hit
CCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAATG111010.10264484541368203No Hit
GTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTA110500.10217327644547215No Hit
CCCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATA110260.1019513616369028No Hit
CCCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACC109450.10120239915798125No Hit
GTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCA109430.10118390625726713No Hit
GCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAA108780.10058288698405848No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)