FastQCFastQC Report
Thu 16 Sep 2021
H2HW2AFX3_n01_11241.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HW2AFX3_n01_11241.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9141548
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTA792820.8672710573745279No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGC749800.8202111939903395No Hit
CCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA694620.7598494259396767No Hit
CCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATT630020.6891830573990313No Hit
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAG624660.6833197178420986No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC594960.6508306908195417No Hit
CCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTT569290.6227501075310221No Hit
CCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGG524780.5740603232625372No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACT463390.5069053950162489No Hit
CCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCA452930.4954631316271598No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT449760.49199544759815295No Hit
CTTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAG441410.4828613272062894No Hit
CCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAA427240.4673606702059651No Hit
CACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTT409260.4476922289310301No Hit
CCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAG405200.4432509679979802No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACG399360.4368625532568444No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC392610.42947868347899065No Hit
GCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTT378230.4137483060855776No Hit
CCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGA369150.40381563385107205No Hit
TCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC364770.3990243227952202No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTA358410.39206707660453133No Hit
CCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAA349790.38263760142155356No Hit
CCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAA348300.3810076805372569No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTG336480.36807770412625956No Hit
CGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCT332710.36395367611699897No Hit
CTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATAA328480.35932645105621064No Hit
CCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTC325970.35658074540548274No Hit
GCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGG314940.34451495523515274No Hit
CTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTC305310.33398063435208125No Hit
GCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCC298730.326782728701966No Hit
CCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATA298430.3264545567118392No Hit
GTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACT287060.31401683828603205No Hit
CCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGT281380.3078034486062973No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT280680.30703771396266805No Hit
GTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCA279620.30587817293088654No Hit
CCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAATA277770.3038544456584377No Hit
ACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA271280.2967549916053605No Hit
CCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTATTCTA270240.2956173287062541No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG268420.293626418632818No Hit
CCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACT265660.2906072363236511No Hit
GCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCC265390.29031188153253695No Hit
CCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGAA256230.28029169676733084No Hit
CTCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGT255350.2793290589296255No Hit
CCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCAT251620.27524878718571516No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA251100.274679955736162No Hit
CTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTA245050.26806182060193745No Hit
GCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGT244320.26726326875929546No Hit
GCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGAT238220.26059043829338313No Hit
GCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGA237300.2595840441903275No Hit
CCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAAT233890.2558538225692191No Hit
CCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC232900.2547708550018006No Hit
GCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTATT230450.2520907837490981No Hit
TCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCT229900.2514891351005322No Hit
CCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAG229720.2512922319064561No Hit
CTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCT222840.24376615426621398No Hit
CGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTA222770.24368958080185107No Hit
CCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGT221480.24227844124430567No Hit
CCCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATA218990.23955461372625292No Hit
CCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA218470.23898578227669973No Hit
TTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGA212200.23212698768304887No Hit
CCTTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAA211600.2314706437027952No Hit
CTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACTA211000.2308142997225415No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGC210540.2303111026710137No Hit
CCCAGCACGACGGAGTTTCACAAGATTACCAAGACCTCTCGGCCAAGGTT208150.22769666581633657No Hit
CGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACG206620.22602298866668968No Hit
CCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGACCT205070.2243274333843677No Hit
CGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTAT204580.22379141913382722No Hit
CCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTT194920.2132242810517431No Hit
CCCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGA190030.20787507761267565No Hit
GCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTC189790.20761254002057422No Hit
CGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTA188660.2063764255244298No Hit
GCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC183370.2005896594318599No Hit
TCCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACC182540.19968171692584233No Hit
GTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCAC181870.1989487994812257No Hit
CTCCTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTG181230.1982486992356218No Hit
CCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGT179440.19629060636119833No Hit
GTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTAC178690.19547017638588127No Hit
TCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTC174990.1914227218409836No Hit
CCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGCC174600.19099609825381872No Hit
CAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCAC173730.1900443994824509No Hit
CCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGT173050.18930054297149673No Hit
CCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTT170360.18635793412669277No Hit
CCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGAC169630.1855593822840508No Hit
CCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAAT168440.18425763338988102No Hit
CCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCG168360.18417012085918052No Hit
CCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCG168210.1840060348641171No Hit
ACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTT166990.18267146877093465No Hit
CTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAG166620.18226672331644486No Hit
GCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGCAGTCCTCA166200.1818072825302673No Hit
GCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCC165640.18119469481536388No Hit
GCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAA165470.18100873068762532No Hit
CCCTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAA162750.1780333046438087No Hit
CTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAAC159610.17459843781381446No Hit
GCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCA158260.1731216638582437No Hit
GCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA158230.17308884665923102No Hit
CCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCC155890.1705291051362417No Hit
CCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCGC154090.1685600731954807No Hit
ACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTT153590.16801311987860262No Hit
CCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAA150300.16441416705354497No Hit
CCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCC150180.16428289825749426No Hit
ACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAA149720.16377970120596644No Hit
GCCGACTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTT148440.1623795007147586No Hit
CCTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAA148400.16233574444940835No Hit
CCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTAAAAGCTCTTCA145770.15945877000262976No Hit
TTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAGG144470.1580366913787468No Hit
CTCCACATTCAGTTACGTTACCGTGAAGAATCCATATCCAGGTTCCGGAA142070.1554113154577321No Hit
CCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTC141980.15531286386069404No Hit
TCCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAG138960.15200926582675056No Hit
TCCCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGG138730.15175766730098667No Hit
CCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA137360.15025901521274077No Hit
CCCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAG135710.1484540692670432No Hit
CCCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGATCGGT135470.14819153167494173No Hit
CATAAAACTGATACGAGCTTCTGCTATCCTGAGGGAAACTTCGGCAGGAA134430.14705386877583534No Hit
ACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGAC133850.14641940292825678No Hit
GCTACTACCACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGG133510.1460474746727797No Hit
CTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTATC131020.14332364715472695No Hit
TTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGC127800.13980126779403224No Hit
TCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAG127470.13944027860489275No Hit
ACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCA127270.1392214972781415No Hit
TGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCC126510.13839012823648686No Hit
CTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTGAGCT125120.13686959801556586No Hit
CGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCA124780.13649766976008879No Hit
CTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGG122830.13436455182426432No Hit
GCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC122400.1338941719717492No Hit
CGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGCC122350.13383947664006138No Hit
ACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGCC121870.13331440145585846No Hit
CGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCC121410.13281120440433064No Hit
CCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGC121350.1327455700063053No Hit
TACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGC119190.13038273167739206No Hit
CGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTATTT119170.13036085354471694No Hit
CAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCT118810.12996704715656474No Hit
GCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGC118340.12945291103869935No Hit
CCCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTC118210.12931070317631105No Hit
CTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGTT113960.12466159998284754No Hit
GCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTT113330.12397243880358118No Hit
CCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTG111240.1216861739390309No Hit
CCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTTC110800.12120485502017819No Hit
GTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCA109390.11966244666658206No Hit
CCCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACC109110.11935615280913037No Hit
TGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAG108470.11865605256352645No Hit
CGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATT108210.11837163683874985No Hit
CCGCTACGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCA107930.11806534298129813No Hit
CCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAGAA107270.11734336460301908No Hit
GTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAGGTGCTG106400.11639166583165127No Hit
TCCCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGAT105300.11518836853451954No Hit
CACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCT104060.11383192430866194No Hit
TTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTT103400.11310994593038291No Hit
GCCCAGTGAAATGCGAGATTCCCCTACCCACAAGGAGCAGAGGGCACAAA102400.11201603929662679No Hit
CACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCGC101990.11156753757678678No Hit
CTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCA101050.11053926534105601No Hit
CTCTTACTCAAATCCATCCGAAGACATCAGGATCGGTCGATTGTGCACCT100290.10970789629940138No Hit
TGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATT100130.1095328712380004No Hit
TGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT100080.10947817590631259No Hit
GGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAA99390.10872338032902086No Hit
GCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAA99250.10857023340029501No Hit
CGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT95440.10440244912568418No Hit
GCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGCCA95140.10407427713555735No Hit
TGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACT94720.10361483634937978No Hit
ACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAG92150.10080349630062656No Hit
GCGTGTATTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCA91600.10020184765206068No Hit
CCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCAT91450.10003776165699726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)