FastQCFastQC Report
Thu 16 Sep 2021
H2HW2AFX3_n01_11222.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HW2AFX3_n01_11222.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9409635
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAG1371011.4570278230770906No Hit
CCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTA1089511.157866378451449No Hit
CCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTT811010.8618931552605389No Hit
CCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAA688230.7314098793417598No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT672140.7143103850468163No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGC651030.6918759335510888No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC648950.6896654333563417No Hit
CAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCAC614900.6534791200721388No Hit
CCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATT614750.6533197090004023No Hit
CCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA579200.6155392849988337No Hit
CTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTC578910.615231090260143No Hit
CCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGG562200.5974726968686883No Hit
CCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCA537260.5709679493412869No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC535990.5696182689339172No Hit
TCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC482420.5126872615143946No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA474800.5045891790701764No Hit
GCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTT450830.47911528980667156No Hit
CTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTA447250.4753106788945586No Hit
CTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCT445770.47373782298675776No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACG414800.4408247503755459No Hit
CCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAAT404990.43039926628397385No Hit
TTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGA399420.4244798018201556No Hit
CGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTA383900.4079860695978112No Hit
CTCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGT374970.3984957971270937No Hit
CCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGACCT369310.3924806860202335No Hit
TACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGC362160.38488209160079No Hit
GTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACT346010.36771883287715196No Hit
CTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATAA339500.3608003923637846No Hit
CGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCT335920.3569957814516716No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACT327770.3483344465539843No Hit
ACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCA311040.3305547983529648No Hit
CTTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAG308270.32761100722822933No Hit
CTCCTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTG308250.3275897524186645No Hit
GTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCA299280.31805697032881725No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTA298540.31727054237491675No Hit
CCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGAC296300.3148900037036506No Hit
CACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTT293760.3121906428889112No Hit
CTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAAC292170.31050088552850347No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG284490.3023390386555908No Hit
GCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAA279100.2966108674778565No Hit
TCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCT260270.2765994642725249No Hit
TTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTT254460.2704249420939282No Hit
CCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAATA253210.2690965164961234No Hit
CCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGAA251560.26734299470702105No Hit
GTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTAC246390.2618486264345004No Hit
CCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACT246070.26150854948146235No Hit
GCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCC244960.26032890755061167No Hit
CCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC239360.2543775608724462No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTG237120.2519970222011799No Hit
GTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCAC237070.2519438851772678No Hit
CCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA230810.24529112978346132No Hit
CTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTATC230440.2448979158065111No Hit
CCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCC223200.23720367474402565No Hit
CCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTATTCTA222360.23631097274230084No Hit
CCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGT218880.23261263587801229No Hit
CCCTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAA217920.2315924050188982No Hit
TCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTC217030.23064656599326117No Hit
CCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCAT213380.22676756324767114No Hit
GCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCA209830.22299483454990549No Hit
GCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTATT209600.2227504042399094No Hit
GCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGG205840.21875450004171254No Hit
AAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACC201690.21434412705700062No Hit
CCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAA201490.21413157896135188No Hit
CCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATA201050.21366397315092456No Hit
CCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAG192340.2044075035854207No Hit
GCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCC192080.2041311910610773No Hit
CGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTA188030.19982709212418973No Hit
CCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGT181970.19338688482603203No Hit
CTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGACAT176990.18809443724437772No Hit
CCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTC176660.18774373288655724No Hit
TTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGC175910.18694667752787436No Hit
CCTTCCTGTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAA175190.1861815043835388No Hit
CTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTTCA174080.18500186245268813No Hit
CCCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATA172910.1837584560931428No Hit
CCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAA170130.18080403756362495No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT168510.17908239798886993No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGC164820.17516088562415014No Hit
CCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTT163940.17422567400329556No Hit
TTGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAAT161700.17184513533202936No Hit
CGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTAT160620.170697375615526No Hit
CCCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGA158580.16852938503990855No Hit
CCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGA157920.16782797632426763No Hit
ACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTT156560.166382649273856No Hit
CCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCGC156380.1661913559877721No Hit
CTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGA155720.16548994727213118No Hit
GCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTC155510.16526677177169996No Hit
GCCGACTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTT153670.16331132929173128No Hit
GGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAA152870.16246113690913622No Hit
GCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCC151650.16116459352567872No Hit
CTCCACATTCAGTTACGTTACCGTGAAGAATCCATATCCAGGTTCCGGAA151570.16107957428741923No Hit
GCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGT151460.16096267283481241No Hit
CCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTTC151330.1608245165726407No Hit
GCGGTAACATTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA150990.1604631848100378No Hit
GCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC149640.15902848516440862No Hit
TGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAA148340.1576469225426916No Hit
TGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT147490.15674359313618433No Hit
CCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAG147150.15638226137358144No Hit
CCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCG147140.15637163396879902No Hit
CTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCGCA145490.15461811217969665No Hit
CCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTAAAAGCTCTTCA145160.1542674078218762No Hit
ACCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAA144160.15320466734363233No Hit
CGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCC143160.15214192686538852No Hit
CGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGT143100.15207816243669386No Hit
GCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGAC143020.15199314319843438No Hit
CCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCAT142410.1513448715067056No Hit
CCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTC141570.1504521695049808No Hit
ATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCA139380.1481247678576268No Hit
GTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTATACCAT138100.14676446004547467No Hit
ACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAG137540.14616932537765812No Hit
CCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGT135390.14388443334943385No Hit
GCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGAT135210.14369314006334996No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG132710.14103628886774033No Hit
TGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATT131590.13984601953210724No Hit
TGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT129260.1373698342177991No Hit
TCCCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGG129230.13733795200345178No Hit
CTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAG128430.1364877596208567No Hit
CCCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCG128250.13629646633477283No Hit
CCTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAA128160.13620081969173087No Hit
CCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAATG127420.13541439173783043No Hit
CTCACTACCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTA127290.13527623547565873No Hit
CCTCACTACCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCT124540.13235369916048817No Hit
GCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTTATTGATA124470.1322793073270111No Hit
GCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGC123380.1311209202057253No Hit
GTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCA123330.1310677831818131No Hit
CCTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAA123280.13101464615790093No Hit
CCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGCC123030.13074896103833997No Hit
CGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT122390.1300688071322639No Hit
CGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCA121970.1296224561314015No Hit
CGCGTCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT114110.12126931597240488No Hit
ACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTT113550.12067418130458833No Hit
CAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAG113130.12022783030372591No Hit
CCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTT112490.11954767639764986No Hit
CCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCCG112110.1191438350159172No Hit
TGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAG111200.1181767411807153No Hit
TTCCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGAC110090.11699709924986464No Hit
CCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAGAA110040.11694396222595245No Hit
CCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTTC109590.11646572901074272No Hit
CGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACG109350.11621067129596419No Hit
CAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAAA108260.1150522841746784No Hit
TCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTT108020.11479722645989988No Hit
CTCTTACTCAAATCCATCCGAAGACATCAGGATCGGTCGATTGTGCACCT105890.11253358924124049No Hit
CACTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGC103570.11006803133171478No Hit
CGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATT103230.10970669956911189No Hit
GCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTT103140.10961105292606993No Hit
CAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCT102380.1088033701626046No Hit
TCCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACC100700.10701796615915495No Hit
CTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTGAGCT100610.10692231951611302No Hit
CCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAAT99590.10583832422830428No Hit
CCCAGCACGACGGAGTTTCACAAGATTACCAAGACCTCTCGGCCAAGGTT96750.10282014127009177No Hit
TGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT96680.1027457494366147No Hit
CTTCCCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGT96290.10233128065009961No Hit
CGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTTATTGAT96260.10229939843575228No Hit
ACCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA96090.10211873255445085No Hit
GCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAA95870.10188492964923719No Hit
CCCTCACTACCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATC95670.10167238155358843No Hit
CCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAATGA94920.10087532619490554No Hit
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC94620.10055650405143238No Hit
CTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGA94470.1003970929796958No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)