FastQCFastQC Report
Fri 16 Jul 2021
H2HN2AFX3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HN2AFX3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91651278
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTCATA2310212725.206551947917188No Hit
TCGATATC2256189824.61711226765436No Hit
CGTCTGCG1973385621.531457531885152No Hit
CTAGCGCT1884024420.556444395679893No Hit
AGATCTCG60424156.592832235247173Illumina Single End PCR Primer 1 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)