FastQCFastQC Report
Wed 4 Aug 2021
H2HMTAFX3_n01_AH7797-Pep4658-Input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMTAFX3_n01_AH7797-Pep4658-Input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7146446
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1134701.5877822346940005TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC830021.1614444438536302TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGC382370.5350491698950779TruSeq Adapter, Index 5 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC161620.22615437099783586TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGGATGC149770.2095727022914607TruSeq Adapter, Index 5 (97% over 41bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)