FastQCFastQC Report
Wed 4 Aug 2021
H2HMTAFX3_n01_9251-SPKIN-antiRED1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMTAFX3_n01_9251-SPKIN-antiRED1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6350248
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC979381.542270475105854TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC195480.3078304973286083TruSeq Adapter, Index 3 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)