FastQCFastQC Report
Wed 4 Aug 2021
H2HMTAFX3_n01_9120-SPKIN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMTAFX3_n01_9120-SPKIN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6833885
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC417710.6112335808987128TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC229250.3354607225611786TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGATGC158200.23149350625595835TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTGG145220.21249991768957188TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATGG106980.1565434595402176TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATGC95330.13949605531846088TruSeq Adapter, Index 8 (97% over 42bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)