FastQCFastQC Report
Wed 4 Aug 2021
H2HMTAFX3_n01_7797-SPKIN-antiRED1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMTAFX3_n01_7797-SPKIN-antiRED1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8608264
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT2174312.5258402855674498TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTAT280920.3263375751487176TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGGGT120700.14021410124038947TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGGAT109330.1270058632030802TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTTT100790.11708516374497807TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGGAT97080.11277535168531076TruSeq Adapter, Index 14 (97% over 45bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)