FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_Neg.7.4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_Neg.7.4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATTGATTGTTTGATTGTAATGTCTATGATTCTCTAACTTCAGCCCAG10.78125No Hit
TATCTACACACACCTACACACATCCACACCTACACCCATACACACAAAAA10.78125No Hit
ACTTACCATTTTCTTGTTAAAATATTAAAAATCAAATCAAATGACAATGA10.78125No Hit
CCTTTATTGAATCGATTTACACCTACAATCTAGCTGGCACTGTACCCTTT10.78125No Hit
ATATAGGGAAATACCTATAATCATACTCAATATGGCATGTGAGATTTAGT10.78125No Hit
GCCTACTACAGCATCCACCAGCTTACCACAAATTATCCTCCCTACTCATC10.78125No Hit
CATCTCAAACATATACCCAGAAAGAATGGTCTATAGATATTCCACAATCA10.78125No Hit
CTATTGTTATGCCCTTACTTTTTTTTCAATGTAAATGATTTTGATTGCAT10.78125No Hit
AATGAGCACCTACCATGCACCTCGGATGAGGTGGGAGTTTTCCATAATCC10.78125No Hit
GAATAAAGGTATTTAGTTTTTTAAGAGCTTTTTACTGGTATACTATTTTC10.78125No Hit
CCTCAATCCTACAAAAGCAAAAATCTTCCTATAGAAACAACACAGAATCA10.78125No Hit
GTTGAGGTGTTTGATAGTGTGTTTCTAGATTGAGTTCTGTTTTCGATGAA10.78125No Hit
GCACTGTGAGCGGCCGGCCGGCGCCGCCGGCCCCCGGCAGTGAGGGGCTT10.78125No Hit
GAATACTCTTACATGCTTTACTCTCTTTTCTAGCTAAATATTTACTTAGT10.78125No Hit
CATCCTCCCTCTGGTTTCACTATCTGGTGTGATCGCCATTTCCATGATAT10.78125No Hit
CTGTGATATTGAACATAAGATAAACTAGGGGAGAGGATAAAATTACTGCC10.78125No Hit
GTCCTCATCCTGCCCTGATCCTGTTTTTCCATGTTCTTCCTTCCAGTCTG10.78125No Hit
CCCTTGTGCTTGCATTCATATTATTTATCAATCTGCATTTACAGGAATAT10.78125No Hit
GGTTAATTGATATGAACAAGAGTCACAAAAGCATCACCAAATTTCTAAAT10.78125No Hit
CCTGTACATCGACTAATACGTATTTCACCATTTTAACATCCGGCACCTAG10.78125No Hit
ACAAGTAACTGTTATCTCTGCCAATTCATCCGATAAAGTCTGCAAAACAG10.78125No Hit
GGTTAGCCCTAAAACCATAAAAACCCTAGAAGAAAACCTAGTCCATACCA10.78125No Hit
GGCCACCATAGTGAAATCCACACGCTACTAAAAATAGAAAAAATTAGGTG10.78125No Hit
GCCGTTTTGTGAAGCAAATGACGCTTAATCTTCAGGCCCCTTTCCAAGAC10.78125No Hit
ATTTATTACTGGCATAAAAAGTGAATTAATAGGGTGTTTAGGGAGATAGA10.78125No Hit
ACTGTGACGATTCTGAACAATGTGCTGACCGTGCTGATCTACCTGACCAG10.78125No Hit
ATTAAATATAGCACGTAAACGTAAAATTACAGCCATTAAAATTTTAGATT10.78125No Hit
CCATCTCGTTAACCCACCCGCATTTTTTTTTTTTACGGCGGAGATGCGCA10.78125No Hit
CCAGTGGGACATCCTGAGAGGAATCTTGGGTCCTGGGCAGCAGGACCTCC10.78125No Hit
GAACTAGACAATTCTAGTTATGCTGCACAAAAAGTTTGAGAACTAAAACA10.78125No Hit
GGTGGAGAAAGGCCGAAAGATGGTAGGAGGCAATTAGATTAATTAGGTAA10.78125No Hit
AGGCTGCAGTCAGCCAAGCTCTCTGCACAACACTAAAGAATTGTCAACAG10.78125No Hit
GTTAATGTGATTGCGAACATGACATTGTCGTTGTCTTAAAGTTGTGTGTT10.78125No Hit
AGCCGAGGTGGGAGGATCACCTGAGGTCGGGAGTTCAAGACCAGCCTGAC10.78125No Hit
CAGAGGCAGACCCTGTCTCAAAAAATAAAAATAAATTTTAAAAAATTGAA10.78125No Hit
GGTATGAGCCATCAAGCAAAGACTTCTTTGCAAATATTAGGATGACTGTA10.78125No Hit
CCTTGCTCCTCATGGTACTTCACCTTGTGATTGTGTGAGTCAATTCTCCT10.78125No Hit
CTGCAGGCCCATGCCAAGCTGCCCACAATTCCCCCTCACTCTCTCCTGTG10.78125No Hit
ATAATATTATGCCTATAGAAATTATCAAACTTATTTAAAGTAAATTAATA10.78125No Hit
GACCAGGCCGGCAGTGCTTTGGTTGGAGGGGAGGGCCTCCCAGCGCCGGG10.78125No Hit
TATCTAAATTAAACAAGGTAAAGCAACAATAACATTGAAAAAGAGTATCA10.78125No Hit
TTGGTAGAGGATACAATACAATTTCATCATTTAAAAAATATTAAAGATTC10.78125No Hit
CCATATATCCACTTGCAGACTTTACAAACAGTGTGTTTCCAAACTCCTCT10.78125No Hit
GTGTAAATACACACTGGATTATTTTGAAGACTTAGCACAATAGATAGAAT10.78125No Hit
GGAAAAGACAAATACATGAAAATATTTGGGGAAAATTTTGCTTTAAAAGG10.78125No Hit
AAAAGTTTGGAAACAGGTGAAACCACACTTCAACCAAAAAAACAAGACAC10.78125No Hit
GTATGTCACGTTGAGGGAAAAGTTAAGATAAAGGTTTGTTTCTACATTTC10.78125No Hit
GCCATGGCCCTTTACTACCGTCTTCCCTGGATTTGGAAACCTCAGATAGT10.78125No Hit
CTTCCTTACTAACAACATGCCCCTGCGAGACTCAAAACCATTTCTTTCAT10.78125No Hit
GAATAAAAGTGTTGAAAGTCTGCATACAAGTCATCTTCCAGATATTGTAT10.78125No Hit
TTCCACCATGAGTGGGAGCAGCCTGAACCCTTCACCAGAAGCAGATGTTG10.78125No Hit
CATCAGCACAAACCCAATAAATTTCTGCCAATAATCCAACCCAGAGCTCA10.78125No Hit
CGCACACGCACTATCAAGAAACAACAGTGATAAAACTAGGAACAAAATGC10.78125No Hit
TTCCAGGCCTAGAGTGCTACTCTGTTTTCCTAGGAAAATCCCCCCTCCAC10.78125No Hit
AAAATGGAAACACATAGCAAACAGCATAAATAATAAGGTCTCTGAGAAAA10.78125No Hit
GTCAAATGTAAGTTCCAAAAAGATAATTATGAAAATTGGTGTGTATAGTT10.78125No Hit
ATATTCAAACTCTAGAAAGAATCAATTAATATCAATACAATATCAGTTTT10.78125No Hit
CCTCAGCACAGTTAATACTTTTACTCAATGATGAGGACAGCTTCATCCCA10.78125No Hit
GTGTGAGTCCAAAAACGCAAAAAAGAGGAAAGAAAGGAGAAACTGAGGCA10.78125No Hit
TAGACATATAGTTATTAAATTGCAGAGCAACATATCAATATATAAAATAT10.78125No Hit
TTGTTTTACTAAGTAGAGTTGAATTTAATAAAAAATTAAAGAAAGTTGTA10.78125No Hit
ATGTTGTTCTCACCGGAGCATGCTTCGTCGGCGTTCACCTTCACGGGAAC10.78125No Hit
ATACAAGATATACTGTATCAAAAATTAACACTGTCGCCGTTTGTAGAACC10.78125No Hit
GGCCAACCAAGTATGCAAAGAACAGAAAAGATAAAAAATATAGATCCGGA10.78125No Hit
GTCTCAAGGTGCTCCAAAATATACGTTTGCAAATATTATAAACACAGTGT10.78125No Hit
GTTTTAAATACCGTAATATGCTGTACAGGTTTGGAGCCTAGTCGCAAGAT10.78125No Hit
CTCAAAAAAATAGCAAATAGACATATTAAATAAAAAAATAATATTTAAAC10.78125No Hit
CTCTAGGATTAGGGAATTGTATACGTGGGTGTCACAGCACAGCGAAGTCA10.78125No Hit
TTATGAGGCAGTGATAAGTAAGTTTCCTAACATAGTGGGTCAGCTCACTC10.78125No Hit
GCACAATGTTGAGTACTTTATTTAGAGCTTGATTAATCCTCAGAATAACT10.78125No Hit
TTTGTGGTACATATGTCTACATTTGAAATGTTTTTTTGATGAAAAAATTA10.78125No Hit
AAATAAATTTAAAAAAAAATAAGAAAAGAATAAAAAAAAAATTCAATAAA10.78125No Hit
AGAGAGCCAGATCTCACTCGGAGGAAAGGCCATGTGAGGACAGAGTGGAT10.78125No Hit
TATTTGCATTTAATAAAACATTGATATTTTATTTTCTTTCTCTTCATTTT10.78125No Hit
CTTCTAAGTATAAGTATGATATAGTAGAACCGCAACGAGTATATCTGAGA10.78125No Hit
ATCATTGCTTGCACCAGCAATAGACATGGATCTGCTCATATACACTGGAT10.78125No Hit
AATTACTTGTACTTTTGAAATAATTATTAAATCTTGATACTGTATTTCAT10.78125No Hit
GATTAGCAATTGCAGCAAAGAAATTTATGTTTGTTAGTTATGTGTCTCTC10.78125No Hit
GTATATAATATATCGTTTCGGTACTTAGCTATTATATGAACAGCTAAGAA10.78125No Hit
AACTAATACCATGTGATAAAAGATTAATTAAAGCAGTGTGTGTATATATA10.78125No Hit
AGTGGTCGATTTGCCAGCTGCACTAGACGGTGTCAACCATTGACCACCCG10.78125No Hit
GAAAAAATGCATGAAAAAAATAATGTTATATTAATTGCTAAACTCTGCTA10.78125No Hit
TCAATCACGCTCTCCAGTCCCGCAGCGTGATCTCTGGTCTCCTGCTGTTA10.78125No Hit
GAGAAAGGACAAACCTCTTCTGAAGCTCACTGACTATGAGCTAAGATACA10.78125No Hit
CCCCAGCCGGCCGTGGGCTAGCCACGCCGGGCGCTCGGCCCCCGCCGCCT10.78125No Hit
GTTCTAGGCTTCACTTGGCATCTGGAATAAACACACACGTAGCTGCTTCT10.78125No Hit
GAAATCAACTTAAACAATATGCTACAACAATATTTCACTCAACGCATTCA10.78125No Hit
GCACAATTGCGTTCTGCTCATGTTAAAGATATTCGTGATCGAGCGGTAAT10.78125No Hit
ACTCAAATCATTATCTAGAGGTTAATGAAGACCAAATAAAGCCCCCAAGA10.78125No Hit
GCTAAGTATGTGGACTCATGATTTGTAATTCTGGGAAGTGTTCTTTATTA10.78125No Hit
CCACATTGGGAGGTACATCAACTATAAAAATTAATTGTACAGACTCTGAT10.78125No Hit
GTCTCCAACTTCATCAGAGTCCTCCCTCACATCCCCATTCCAAGTTTCAG10.78125No Hit
GAGTGAAATTGAATGCAGTGGACACAAATGGAATGGACTTGAATGGAATG10.78125No Hit
GAGTTGAGAAAGGTGGTGCCTGGCGCGGGCTTGCGCCTCAAGCCTGGTCC10.78125No Hit
TGAATCTACTCAGCTAACAGAGTTGAACGTTTCCTTTGAGAGAGCAGGTG10.78125No Hit
GTAGAGACTTCTTAAATAGTTTTGATTAGAATGCTGACAGAAGGAAGGCC10.78125No Hit
GTCCAAACGTTTGTGGTCGAACGGAAATGAATTTATTTGCTCTTTTCTGT10.78125No Hit
GGACCTTGGCACCCCTCCATGCTGCCCCCTCCTCTTCACTGGCACATGGC10.78125No Hit
AGCCTTGTTAACTATATCATTAATGAATATTAAATAACAGTAGCATGCAA10.78125No Hit
TGCCAATCATAAATTGTATTATATAGAATTTCAATCAGTTACCCACTTGA10.78125No Hit
GTATACTTGAATAAAAAACAAATTTTACTTTCTACTGAAGAGATCAAGAA10.78125No Hit
GTAACATATTGCTTACCTTTCCTCATGATTTCAGTGTAGAACCTGTAAGT10.78125No Hit
CATCAAAACAAGAATGTTACAATTGTCTTTCAACCATCAAATCTTATTTT10.78125No Hit
CAGCTAGCCCGCCACGATTTTAACTCCCTGGGAAAGTACTCCGCCCGACC10.78125No Hit
AAAATATCAATACAAATAAGTAAAAAATCCCGAGTTAGTTGATGCTGATG10.78125No Hit
ACCTAGAGGTTCTCTTAAAATGGGACCAGAATCATCATTTTTTAGAGAAA10.78125No Hit
GAATACTATAACAGAAATGGCAATTAGAGAAGTAGCACCTGTCTCTTATA10.78125No Hit
GATCTGGGCTCCCTGCCCCCTCTGCCTCCAGGTTCAAGTGCTTATAATGC10.78125No Hit
TAACAGAACTTATATAATAGGAAAATTACATCCGGCAAAAACTCGAACTG10.78125No Hit
AAACATCTCCAAGCCCATGATTCTTAAACTGTAATATTAGAAATGAAACA10.78125No Hit
GGGCTTTCCTTGTTTGTTTCATTGATACTTACAGAAAGACCTCCTACTCT10.78125No Hit
GGAGAGCAGTGGCGCAATCTCGGCTCACTGCAAGCTCTGCCTCCCGAGTT10.78125No Hit
GTGTGCACTATTCCAGCTATTCTGCAAAGTGCTAGGATTACTGCATTTAT10.78125No Hit
GTACCTATCACTTTAAGGAATAATAACAATAAAAACACAAAAAAACTACA10.78125No Hit
GGATTATAAATCATGCTGCTATAAAGACACATGCTATAAAGACACATATG10.78125No Hit
ATATAAAGGATGTCCCAAAAGATAATAACTGAGCTGGCTTGGTCAAGTTG10.78125No Hit
CGTAACCAGAGACTAGATTGAATGATAGAATGAATATGTATGATAGCAAG10.78125No Hit
GTGCTGATTGGTGTGTTTACAAACCTTGAGCTAGATACAGAGTGCCGATT10.78125No Hit
GGACAGGCATTGGAGTAAAATGAACTTCACTATAGTGGGATAGAGTGGTT10.78125No Hit
AATTGAAACTGGCAGTCATTGGAATTTAGACTCTTATTTATTGATTGTGC10.78125No Hit
GCTCTAGTGATAGTATATAATGTTGAGTATATATGTTTATTAAAAGTACC10.78125No Hit
AACTTATACATAGCATCATTGGTTTTTTGGGAGTTTTTTTATTGTTTGCC10.78125No Hit
GAGTTGAACATTGCCTTTCATAGAGCGGGTTTGAAACGCTCTTTTTGTAG10.78125No Hit
CAGCTGAACTGTGTTCTCATCTGTGGTTGGGGGCTATTTGCAAGTCCCCG10.78125No Hit
GAAGATTCAATTAATTCAGTGAAAATATTATAATACCCTCCACCACATAA10.78125No Hit
AGAATTACAAGATATTTACCTATACAACAGACACATAACCTAAAAAACAG10.78125No Hit
TTCCAAAGAAGGTGCAACTGAATCTATTTAGATGATTTATGGATTGCATA10.78125No Hit
TCTTAAAGACATATAAGAAGTAAAAAAAACGCTGGACTCGCTGGCAAACC10.78125No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers