FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_Neg.7.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_Neg.7.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5855
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1612.7497865072587535No Hit
CGTGGGCTCGGAGATGTGTATAAGCTGTCTCTTATACACATCTGACGCTG60.10247651579846286No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTACA100.006912936145.04
CAACCTG100.006912936145.09
ACCGCCC100.006912936145.08
CTGGTAC100.006912936145.03
TGTATCT100.006912936145.0145
GGTACAA100.006912936145.05
CCTGGTA100.006912936145.02
Produced by FastQC (version 0.11.5)