FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_Neg.7.10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_Neg.7.10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4591
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1753.811805706817687No Hit
GTCGAGATCATGCCACTGCACTCCAGTCTGAGCGACAGAGAGAGACCCTG50.10890873448050534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT100.0068621617145.02
CCGTCCC100.0068621617145.02
CCCGTCC100.0068621617145.01
GTCCCCT100.0068621617145.04