FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_9424.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_9424.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25135995
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC89700.027.812773145
GCCGACC43900.019.163366145
TATACTG304700.015.6320415
TACACTG226200.015.4481755
GACGACA49350.015.136602145
CTATACT184350.014.0799654
CTACACT166800.013.561894
CCTATAC97900.013.3356423
TCTATAC182250.013.0934013
CTTACAC162750.013.0578223
TACACAG279600.013.0422815
TCTACAC156750.013.0023813
GTGTACG26850.012.98549651
CCTACAC120300.012.9024533
GTGTTAG193300.012.8515611
GTATAGA190000.012.5777781
CTAGGAC117850.012.555233
GTGTAAG166300.012.4920991
GTGTAGG114050.012.419411
GTATAAG170300.012.4119481
Produced by FastQC (version 0.11.5)