FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_7920.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_7920.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24023114
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG223350.016.9431365
TATACTG315700.015.9135825
GTGTAGG113850.015.8224911
CCTACAC116500.014.7547693
CTTACAC155950.014.5103953
CTATACT188600.014.4923194
TACACCG24300.014.3200415
TCTATAC182900.014.1567813
GTATAGG96550.013.84273051
CTACACT161350.013.614824
GTATAGA185500.013.1959971
TGACGCG16600.013.107409145
TATACAG249900.013.025355
TACACAG274350.012.9743575
GTGTAAG163700.012.9565811
TACGTCG5601.1834554E-512.9455735
GTAGGAC73250.012.8719913
GTATTAC142550.012.7897691
ATACTGT387900.012.7454956
TCTACAC150950.012.6366693
Produced by FastQC (version 0.11.5)