FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_7784.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_7784.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29311470
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG312570.10663743578878848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCC65650.020.769186145
GCCGACC72650.019.866158145
TGACGCG32900.016.974274145
CGACGAA68650.015.001828145
TACACCG35900.014.7412775
TATACTG331900.014.5251645
TACACTG245150.014.3717975
CGCCGTT39650.013.718747145
GTGTAGG126950.013.5609691
CGGTGGG113950.013.556939145
CTGCCGC312300.013.515975145
CTATACT208850.013.4691974
GTATAGG114700.013.4260281
CTACACT178100.013.23012354
TCTATAC208100.013.1397513
GTATTAG199050.012.9186221
TCTACAC180550.012.6942623
TCTCGGG72700.012.669681145
CCGTATA53450.012.619193145
GACTCCG123500.012.5024637
Produced by FastQC (version 0.11.5)