FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_6331.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_6331.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34793941
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG389500.11194477797154395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC76200.015.704632145
ACGAACG35550.015.301023145
TATACTG395150.014.8054995
CTGCCGC258200.013.623361145
CTATACT258500.013.4907664
TACACTG289350.013.4543375
GTATAGA256300.013.40553951
CCGACGC73200.013.078611145
CTACACT211650.012.9486834
GTGTAGG164300.012.9097261
GTATAGG139250.012.7803481
CCTATAC138250.012.5903923
CCTACAC157350.012.5831393
GTGTAAG228350.012.183431
GTGTACG42400.012.1636611
GTATTAG242600.012.0965731
TCTATAC244950.011.9618153
TAGACTG235000.011.9386335
GTGTTAG258550.011.8560421
TAGACAG448500.011.5895945
Produced by FastQC (version 0.11.5)