FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_6119.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_6119.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23917790
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG305350.12766647754662952No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC105100.018.631405145
TATACTG254100.015.8915155
TACACCG53150.015.5494995
GCCGACC94000.014.736357145
CTATACT163750.014.6550974
CTACACT142150.013.92384
GTATAGA167900.013.0227061
GTATTAG172800.012.9897321
GACGACA72900.012.933033145
CTAGACT118500.012.9094024
CTTACAC142950.012.8868263
TCTATAC160300.012.8493513
GTCTAGG101350.012.7579731
GTGTAGG114650.012.6718891
TACACTG185250.012.5620815
TCTACAC155100.012.5319713
CCTATAC90400.012.1144843
GTATAGG91850.012.1003441
TACACAG234600.011.9899815
ATACTGT327300.011.9604636
Produced by FastQC (version 0.11.5)