FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_4340.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_4340.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24941303
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG296310.11880293503511022No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG273200.015.3903825
TACACCG41900.015.0524555
TACGCGG39000.014.319035145
CTACACT153500.014.0273154
TACACTG201500.013.887195
CCTACAC114400.013.7565913
GTGTAGG118200.013.5188721
CTATACT184550.013.5135954
GTATAGG105650.013.2685381
GTATAGA183450.013.18444251
GTCCTAG104150.013.0412021
CTGCCGC203100.012.783821145
GCCGACC68600.012.686585145
GTGTTAG184350.012.5290811
GTATTAG186400.012.4302551
TCTATAC179950.012.4129783
TAGGACT146650.012.4084674
TACACAG249050.012.3418835
GTAGGAC76150.012.2856173
CTAGTAC75600.012.2790663
Produced by FastQC (version 0.11.5)