FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_4329.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_4329.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26091313
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG293050.11231707656874149No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG302250.015.3743775
TACACTG214500.014.6341135
CTATACT193500.014.0502614
TACACCG45550.013.5280875
TCTATAC191500.013.4820273
GTGTAGG120500.013.3812451
GTATAGG110400.013.158051
CTTACAC167650.013.1505533
CTACACT163700.012.887794
GTGTTAG184300.012.7688051
CTGCCGC190850.012.654798145
GTATTAG186400.012.5859841
ATACTGT390100.012.26486
GTATAGA193150.012.2213511
GTGTAAG163850.012.0130541
GTCCTAC84050.012.0117871
CCTACAC125150.011.9953663
TACACAG261250.011.9598795
GTATAAG182250.011.9161011
TAGGACT161150.011.8770264
Produced by FastQC (version 0.11.5)