FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_30982.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_30982.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25740077
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG278490.10819314953875235No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG157200.015.7719815
TATACCG72650.014.868375
TATACTG225600.014.4927275
GTCCTAG69950.014.3301611
TACACCG112000.014.2402415
CTATACT175750.014.0669654
CTACACT139800.013.5355124
GTATAGG114100.013.432491
GTATAGA193100.013.3538281
TACACAG190450.012.8280785
CCTACAC142350.012.7377993
GTATTAG191200.012.7267241
GTCCTAC84250.012.5013761
TCTATAC188200.012.48643
GTGTAGG136000.012.3376591
CTTACAC139400.012.3309763
GTGTAAG138000.012.2641241
TATACAG215250.012.2257685
TCTACAC180950.012.1850373
CCTATAC114100.012.0775773
Produced by FastQC (version 0.11.5)