FastQCFastQC Report
Sat 17 Aug 2019
H2HMJBGXC_n02_MG_30981.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2HMJBGXC_n02_MG_30981.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24896274
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG284050.015.92596155
TACACTG219450.015.2293435
CTACACT159700.015.1178824
CTATACT186550.014.1467854
GTATAGG97550.013.8496511
GTATAGA180050.013.7567051
TACACCG36400.013.742435
GTACTAG81950.013.5611231
CCTACAC117250.013.3611663
TCTATAC185900.013.3038833
GTATTAG180200.013.3018591
TACGCGG25500.013.082757145
CTAGGAC103850.013.059863
GTGTAGG118250.013.0223061
TACACAG269150.012.8481285
GTGTAAG167750.012.5570791
TATACCG29400.012.3293035
CTTACAC164450.012.2606743
GCCGACC55750.012.09817145
GTGTTAG184450.012.0896261
Produced by FastQC (version 0.11.5)